List of Articles پروستات

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  1 - Histopathological study of the effect of pentoxifylline on experimental chronic non-bacterial prostatitis induced by carrageenan in rat
  Hajighorbani, M., Ahmadi-hamedani, M. *, Shahab, E., Hayati, F., Kafshdoozan, K., Ghafari Kalili, S., Jebelli Javan, A. .
  Determination of histopathological criteria of chronic non-bacterial prostatitis (CNP), as the most common type of prostatitis syndrome, is highly effective to better understanding of pathophysiology and finding therapeutic strategies of this disease. Interest in pentox More

  Determination of histopathological criteria of chronic non-bacterial prostatitis (CNP), as the most common type of prostatitis syndrome, is highly effective to better understanding of pathophysiology and finding therapeutic strategies of this disease. Interest in pentoxifylline (PTX) has been renewed because of the anti-inflammatory and antioxidant potential of this drug. The aim of this study was to evaluate histopathological criteria of CNP in rat and to investigate the effectiveness of pentoxifylline on these criteria. Thirty male adult Wistar rats were divided into negative control, CNP control, standard and treatment groups. The negative control group received orally 1 ml saline normal for 21 consecutive days CNP was induced by single intraprostatic injection of 1% carrageenan (100 µl) in CNP control, standard and treatment groups. CNP control, standard and treatment groups received orally 1 ml saline normal, cernilton 100 mg/kg and PTX at 50 and 100 mg/kg 1 week after CNP induction for 21 consecutive days. Results showed that in the CNP control group, the PI was significantly increased as compared to negative control group (P<0.01). A significant reduction in the PI was observed in standard and treatment groups, especially PTX 50 mg/kg, in comparison with the CNP control group (P<0.05). Histopathological studies have shown a considerable improvement in the prostatic histoarchitecture in standard and treatment groups, especially PTX 50 mg/kg, in comparison with the CNP control group. Manuscript profile
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  2 - Evaluation of probiotic effect of Lactobacillus Casei and Bacillus Coagulans on AGS and DU145 cancer cell line
  سمانه Ansarinia علی Sharifzadeh
  Probiotics are a live microbial oral supplement and a range of different beneficial effects have been attributed to probiotics . Today, probiotics are recognized as a factor in preventing many infectious diseases and cancers. In this study, the effect of probioty lactob More

  Probiotics are a live microbial oral supplement and a range of different beneficial effects have been attributed to probiotics . Today, probiotics are recognized as a factor in preventing many infectious diseases and cancers. In this study, the effect of probioty lactobacillus casei and Bacillus coagulase on cancer cells (AGS, DU145) (gastric and prostate cancer cell line) was investigated. To do this, these two bacteria were cultured in Broth MRS medium and from 48, 24 and 72 hour cultures, sediment and supernatant were prepared. Supernatants were prepared in acidic and neutralized forms with 1N NaOH.. Concentrations of 100, 10 and 1000 μg / ml of extracts were also prepared separately on AGS and DU145 cancer cells cultured in 96-well microplate. The results showed that the concentrations of 1000 μg / ml of extract and 300 μl / ml of supernatant in 72 hours had inhibitory concentrations of more than 50%. The inhibitory effect of supernatants was directly related to time and concentration. Manuscript profile
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  3 - Dietary pattern and risk of prostate cancer
  جمال رحمانی محمد عطایی
  Factor analysis was conducted over two dietary patterns were identified. Western dietary pattern with high consumption of sweets, jams and preserves, mayonnaise, soft drinks, salty snacks, solid fats, high fat dairy products, fried potatoes, Ama’ and viscera, meat More

  Factor analysis was conducted over two dietary patterns were identified. Western dietary pattern with high consumption of sweets, jams and preserves, mayonnaise, soft drinks, salty snacks, solid fats, high fat dairy products, fried potatoes, Ama’ and viscera, meat and processed foods, tea and coffee , a healthy diet with a higher intake of refined grains and cooking oils, legumes, nuts, fruits, poultry and fish, whole grains, eggs, vegetables and low-fat dairy Bvd.ps adjusting for the impact of possible turbulence earn higher scores the Western pattern diet significantly (P <0.05) were associated with increased risk of prostate cancer (odds ratio 2.33; 95% CI, 9/4 to 1/1) in front earn a high score of a healthy diet significant relationship (P <0.05) with a reduced risk of prostate cancer (OR = odds of 25/0, 95%, 53/0 to 11/0). lower risk of prostate cancer.People with a diet rich in refined grains close to the Western dietary pattern, red and processed meat and high-fat and fried food more risk of prostate cancer versus those who risk their diet is closer to a healthy diet lower risk of prostate cancer. Manuscript profile
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  4 - Evaluation Of Cytotoxic Effect Of Aqueous andHydroalcoholic Extracts Of Guava Fruit Psidium guajava L on Cell Line DU-145
  Sholeh Zaeemi Baravati کهین شاهانی پور Ramesh Monajemi
  Inroduction and Objective:Prostate cancer is the second most common cancer in men after skin cancer. Guava Fruit with scientific name Psidium guajava L. are considered as an important source of phenolic compounds and antioxidant effect.In this study, the cytotoxic effec More

  Inroduction and Objective:Prostate cancer is the second most common cancer in men after skin cancer. Guava Fruit with scientific name Psidium guajava L. are considered as an important source of phenolic compounds and antioxidant effect.In this study, the cytotoxic effect of aqueous and hydroalcoholic extracts of Guava fruit and effect of the extracts on DU-145 cell line was and its effect on prostate cancer cells was studied. Materials and Methods:Guava fruit was collected from Sistan and Baluchestan region. The aqueous and hydroalcoholic extracts were prepared from this plant. Class of DU-145 cells was cultured in RPMI-1640 medium containing 10% bovine serum in a 5% CO2 incubator, and under different concentrations of aqueous extract of 24, 48 and 72 hours were incubated. MTT assay for cell survival in the presence and absence of extract was used to calculate the percentage and absorbance at 540 nm was measured by ELISA. Data were assessed using SPSS software(* = P-value Results:The results showed that the aqueous and alcoholic extract Guava fruit is a cytotoxic effect. Further studies showed that the cytotoxic effect of aqueous extracts of the ethanol extract is much stronger. Manuscript profile
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  5 - Evaluation of ZEB1 gene expression in cancerous and benign tissue of human prostate cancer
  fahimeh baghbani-arani behnaz karimi azadeh shojaee
  Metastatic Prostate cancer is a lethal disease, but unfortunately the molecular mechanisms that underlie the metastasis of this common cancer are still poorly understood. In advanced stages of the cancer, cells cancerous may spread to the other part of the body speciall More

  Metastatic Prostate cancer is a lethal disease, but unfortunately the molecular mechanisms that underlie the metastasis of this common cancer are still poorly understood. In advanced stages of the cancer, cells cancerous may spread to the other part of the body specially to bones and lymph nodes. One of the important contributing processes in cancer invasion and metastasis is Epithelial to Mesenchymal Transition (EMT). Overexpression of ZEB1 as a key regulating factor, promotes EMT processes in a variety of cancers. In the present study, to evaluate the expression of ZEB1 and its differences between benign prostate hyperplasia and prostate cancer, 16 patients with BPH and 17 patients with Pca were selected, RNA was extracted and after synthesis of cDNA , ZEB1 expression in mRNA level was analyzed by Real Time PCR. Our results indicated that ZEB1 was overexpressed in Pca samples in comparison to BPH ones (P=0.047). In conclusion our findings suggest that evaluating the expression of ZEB1 is likely to be useful as a prognostic biomarker to diagnose prostate cancer in early stages. Furthermore, due to the critical role of ZEB1 in metastasis promotion of a variety of cancers through regulating other EMT markers, it would be possible to avoid progression and metastasis of prostate cancer by suppressing ZEB1. Manuscript profile
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  6 - The cytotoxic effect of Spirulina extract on gastric and prostate cancer cell lines
  F. Khodaverdipour Ali Sharifzadeh
  10.30495/jfh.2022.1955841.1350
  The AGS cell line contains gastric cancer cells, which are among the most common cancers in the world. The DU145 cell line also includes prostate cancer cells, the second leading cause of death in men after lung cancer. This study was designed to assess the cytotoxicity More

  The AGS cell line contains gastric cancer cells, which are among the most common cancers in the world. The DU145 cell line also includes prostate cancer cells, the second leading cause of death in men after lung cancer. This study was designed to assess the cytotoxicity of spirulina extract on AGS and DU145 cell lines. In this in vitro trial, cell lines AGS and DU145 were used. Gastric and prostate cancer cell lines were prepared and cultured from the Iranian Center for Biological and Genetic Resources. The cells were divided into treatment and control groups. The effect of spirulina extract on the treated group was determined using the MTT method. According to MTT results, Spirulina extract has anti-cancer activity and more than 50% reduction in cell density against AGS and DU145 cell lines; While it did not show any significant effect on healthy cells (P< 0.05). The results of this study indicate that Spirulina extract has a good potential to control cancer cells. Manuscript profile
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  7 - The effects of Oleoreupien on prostate and seminal vesicle tissues in streptozotocin induced diabetic rats
  Farzin Javid Esmaeil Safavi Esmaeil Safavi Yoosef Doustar
  10.30495/jvcp.2019.664876
  Diabetes impairs the normal reproductive system function by disrupting spermatogenesis and decreasing sperm count, serum testosterone level and seminal fluid volume. This study aimed to investigate the effects of oleoreupien on tissue changes of prostate and seminal ves More

  Diabetes impairs the normal reproductive system function by disrupting spermatogenesis and decreasing sperm count, serum testosterone level and seminal fluid volume. This study aimed to investigate the effects of oleoreupien on tissue changes of prostate and seminal vesicles in diabetic rats. Thirty male Wistar rats were randomly divided into 3 groups (n=10) including control, diabetic and treatment groups. Rats in the treatment group received Oleoreupien (500mg/kg) for 28 days by oral gavage after induction of diabetes by streptozotocin. At the end of the experimental period, samples of prostate and seminal vesicle were obtained and then slides were prepared and stained by H&E method for histological study. Morphological findings indicated increased secretion of prostate and seminal vesicles, significant increase in diameter of secretory units and significant decrease of interstitial connective tissue in the treatment group compared with the diabetic group. Histomorphometric results also indicated that epithelium height of the prostate and seminal vesicles and diameter of the tubules of the prostate significantly increased in the treatment group compared with the diabetic group (p < /em>˂0.05). The study also revealed that gland weight to body weight in the treatment group increased significantly compared with that of the diabetic group (p < /em>˂0.05). According to current findings, oral administration of Oleoreupien prevented diabetic induced tissue changes in prostate and seminal vesicle in rats. Manuscript profile
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  8 - Complications of ultrasound-guided needle biopsy of the prostate in the dog
  Ali Rezaie Gh Mousavi داریوش Mohajeri Gh Asadnasab نوید Haji Alilo Gh Akrami Nojadeh
           The objective of this research was to investigate and study the clinical, laboratory and pathologic complications following needle biopsy of the prostate. This study was conducted on 10 male dogs. Prior to biopsy, urine and bl More

           The objective of this research was to investigate and study the clinical, laboratory and pathologic complications following needle biopsy of the prostate. This study was conducted on 10 male dogs. Prior to biopsy, urine and blood samples were collected and compared with those taken on days 1, 2, 3, 4, 5, 6, 9, 12 and 15 after the biopsy. The biopsy was done under ultrasound guidance using No. 20 Vim Tru- Cut needle. Fifteen days after the biopsy, the animals were euthanized and their prostate glands collected for pathologic evaluations. Results of the blood examinations were analyzed statistically. Clinically, there were no complications other than edema of the biopsy region. Also, significant hematological alterations were not observed and the increase in the levels of acid phophatase was not statistically significant. Pathologic evaluations revealed capsular and glandular hemorrhage and also the process of healing. In light of the clinical, laboratory and pathologic findings and statistical analysis, it can be stated that the observed complications were limited and this technique does not induce any necrotic complications in the gland and the tissue architecture of the prostate is maintained. Because sufficient tissue samples are obtained for pathologic investigations, this technique provides the possibility for better diagnosis of prostatic diseases.     Manuscript profile
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  9 - Experimental study of apoptosis in the prostate tissue following castration
  یوسف Doustar Ali Rezaie صابر Atash Benab
  The Prostate gland is one of the accessory reproductive glands with important physiological functions necessary for successful reproduction. This gland depends on the presence of sex hormones including androgens for its natural function and normal growth and development More

  The Prostate gland is one of the accessory reproductive glands with important physiological functions necessary for successful reproduction. This gland depends on the presence of sex hormones including androgens for its natural function and normal growth and development. So in the case of hyperplasia, hypertrophy or other prostatic disease the most successful and efficient method of treatment is androgenic control that in some cases is unavoidable. The purpose of this study was to investigate the effects of androgenic depletion states by means of castration on the induction of apoptosis in the epithelial glandular cells of the prostate tissue. Two groups of male dogs each containing 5 animals per group were used in this study. The dogs were under observation for 1 month to detect any possible diseases or disorders. After this period the dogs in the treatment group underwent open castration to decrease the levels of the androgenic hormones in the blood while the dogs in the control group were left intact. One week after surgery, the prostate glands of control and treatment animals were collected and used to prepare microscopic sections. The sections were evaluated following staining with TUNEL (TerminaldeoxyNucleotidyl (dUTP) transferase-mediated End Labeling) and H&E methods. The Mann – Whitney U test was used for statistical analysis. Histopathological studies in the treatment group revealed the presence of various forms of apoptotic cells in the glandular epithelium. Average number of apoptotic cells in ten microscopic fields were significantly higher in the treatment group compared with the control group (p<0.005). These results demonstrated that the type of cell death observed in the prostate gland in androgen deprivation states is Apoptosis.   Manuscript profile
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  10 - Investigate of miR-21 expression level in urine samples of people with prostate cancer
  Alireza Emamvirdizadeh mehrdad hashemi franak jamshidian
  Introduction: Prostate cancer is the most common cancer in men A group of diagnostic biomarkers is MicroRNA, they can be used to diagnose variable diseases, especially cancers. this study, we investigate changes in the expression level of miR-21 in urine, in people with More

  Introduction: Prostate cancer is the most common cancer in men A group of diagnostic biomarkers is MicroRNA, they can be used to diagnose variable diseases, especially cancers. this study, we investigate changes in the expression level of miR-21 in urine, in people with prostate cancer and healthy group. Materials and methods: This research was done on 70 urine samples of prostate cancer patients (32 Metastatic and 38 Non-metastatic) and 30 control samples with negative biopsy report. First RNA was extracted with Trizol, after the cDNA synthesis, changes in the expression of miR-21 and miR-214 in the urine of people with prostate cancer and healthy group were investigated by using Real-time PCR. Statistical analysis of data was calculated with REST 2009. Results: The results revealed the presence of miR-21 in urine samples in patients with prostate cancer, and it was found that miR-21 showed a significant increase in expression (P=0.003) than healthy group. miR-21 in metastatic group (P=0.042) demonstrate over expression than non-metastatic group (P=0.036). Conclusions: The results show that, miR-21 has significant change in expression level in patients with prostate cancer in compare of healthy group, and can be a non-invasive method to detect prostatic patients and also use this biomarker to determine metastatic and non-metastatic group. Manuscript profile
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  11 - Modeling the androgen deprivation therapy in metastatic castration-resistant prostate cancer and identifying the responder and non-responder patients before starting the treatment
  Mahdi Sohrabi-Haghighat Atefeh Deris
  10.30495/jdb.2021.680722
  Androgen deprivation therapy is a common treatment method of metastatic castration resistant prostate cancer disease aimed at slowing down the cancer cells growth by reducing the concentration of androgen in the blood. However, only some patients respond to the androgen More

  Androgen deprivation therapy is a common treatment method of metastatic castration resistant prostate cancer disease aimed at slowing down the cancer cells growth by reducing the concentration of androgen in the blood. However, only some patients respond to the androgen deprivation therapy effectively. In the other patients, the population of cancer cells reduce temporarily, and the cancer recurrence will be observed shortly after the treatment. In this paper, the response mechanism of cancer cells to the androgen deprivation therapy is analyzed by modeling the treatment based on the evolutionary dynamic which is fully supported by clinical observations. The study determines the group of patients on whom the androgen deprivation therapy is effective and the group on whom it is ineffective or even harmful. In the following, by simulating the treatment process under different schedules of abiraterone administration, the results of different treatment methods will be determined and appropriate schedules are discussed. Manuscript profile
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  12 - Investigation of miR-25 expression level in urine samples of people with prostate cancer
  Alireza Emamvirdizadeh ملیحه انتظاری Saghi Nooraei Faranak Jamshidian Hamidreza Gholamrezaei
  10.30495/jdb.2021.686990
  Introduction: After breast cancer, prostate cancer is the most common cancer in men. Screening is recommended by examining prostate-specific antigen levels and performing accurate rectal tests, but there are still problems with the specificity and sensitivity of these t More

  Introduction: After breast cancer, prostate cancer is the most common cancer in men. Screening is recommended by examining prostate-specific antigen levels and performing accurate rectal tests, but there are still problems with the specificity and sensitivity of these tests. A group of diagnostic biomarkers are microRNAs, a class of small non-coding RNAs that play a broad regulatory role in molecular signaling pathways in the cell. The aim of this study was to investigate the changes in the expression level of miR-25 in the urine of patients with prostate cancer (metastatic and non-metastatic groups) and healthy individuals.Materials and methods: 70 urine samples from prostate cancer patients (32 metastatic and 38 non-metastatic) and 30 from healthy subjects with negative biopsy reports were collected. RNA was extracted with Trizol, after the cDNA synthesis, the expression level of miR-25 in the urine was detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Statistical analysis of data was calculated with REST 2009.Results: The results showed that miR-25 significantly reduced expression in patients with prostate cancer compared with healthy individuals (P = 0.001). Also, miR-25 in the metastatic group (p = 0.002) had a lower expression than the non-metastatic group (p = 0.045) compared to healthy individuals.Conclusions: The results of this study showed that miR-25 had a significant decrease in expression in patients with prostate cancer compared to healthy individuals, which with further studies is hoped to be able to diagnose patients with prostate cancer and even distinguish metastatic and non-metastatic groups. Manuscript profile
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  13 - Inhibitory effects of Behind the scenes medicine extract (Physalis alkekengi) on prostate cancer caused by DMBA injection in mice
  Maryam Teimouri fariba khosravinejad
  10.30495/jdb.2021.683731
  Introduction: Although many clinical and pre-clinical studies have widely demonstrated the therapeutic effects of hydroalcoholic extract of the puppet pup on several diseases, there is little information on the therapeutic properties of this plant's hydroalcoholic extra More

  Introduction: Although many clinical and pre-clinical studies have widely demonstrated the therapeutic effects of hydroalcoholic extract of the puppet pup on several diseases, there is little information on the therapeutic properties of this plant's hydroalcoholic extract on prostate cancer. In this study, we evaluated the therapeutic effects of hydroalcoholic extract of Physalis alkekengi extract on prostate cancer induced by injection of 12.7-dimethylbenzanthracene (DMBA) (a carcinogenic drug) on mouse modelMaterials and Methods: After induction of anesthesia, DMBA was injected directly into the prostate gland of male rats and after 14 days, rats received different amounts of puppet extract every 48 hours and by the end of day 21, intraperitoneal injection of puppet extract. In the control group, intraperitoneal injection of normal saline was performed.Results: The results showed that the puppet extract significantly reduced prostate weight, especially at two concentrations (100 mg, 500, and 1000). Histopathological changes were also observed in the prostate tumors of p.alkekengi extract treated groups compared to the control group.Conclusion: It is suspected that the hydroalcoholic extract of the p.alkekengi inhibits abnormal growth, precancerous lesions, and DMBA-induced carcinoma in the BALB / c mouse prostate. Manuscript profile
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  14 - Investigating the combined effect of endostatin along with ‎endometrial stem cells expressing TSP-1 anti-angiogenic gene on ‎angiogenic induce factors on the treatment of prostate cancer in rats
  hossein Attar shakeri Esmail Fattahi Mohammadreza Nowroozi Seyed Ali Momeni Abbas Piryai
  Prostate cancer is the second leading cause of cancer deaths in men. Androgen ‎deprivation therapy has been identified to induce oxidative stress in prostate cancer, ‎leading to reactivation of androgen receptor (AR) signaling. Thus, antioxidant therapies ‎h More

  Prostate cancer is the second leading cause of cancer deaths in men. Androgen ‎deprivation therapy has been identified to induce oxidative stress in prostate cancer, ‎leading to reactivation of androgen receptor (AR) signaling. Thus, antioxidant therapies ‎have gained attention as adjuvants for this cancer. Here, we report for the first time that ‎endostatin along with endometrial stem cells expressing TSP-1 anti-angiogenic gene ‎improved androgen- phenotype and genotype in rats prostate cancer cells.‎‏ ‏Forty male ‎rats (n=8) weighing approximately 95-100 g were purchased from Pasteur Inistitute of ‎Tehran . Animals were suffered to prostate cancer by injecting LNCaP cell line for 12 ‎weeks. Prostate cancer animals were immediately received endostatin for 28 days. ‎Prostate cancer animals were received stem cell containing the Tsp-1 anti-angiogenesis ‎gene. Histological examinations were measured. The levels of IL8 and VEGF serum ‎were assayed.‎‏ ‏In group receiving cells and drug, amount and height of acinus wall folds ‎decreased compared to the patient group and the shape of the vesicles was clear and the ‎epithelial lining were cohesive and regular. The levels of IL8 and VEGF serum were ‎significantly decreased in combination group than to prostate cancer group (P≤0.01). ‎ Manuscript profile
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  15 - The Effect of a NSAID Drug on the Expression of Inflammatory Genes in Prostate Cancer Cells
  Fatemeh Moghbeli Mohammad Morad Farajollahi Abbas Mirshafiey Monireh Mohsenzadegan
  10.22034/ascij.2021.684782
  Inflammation is a physiological process begining with tissue damage in the presence of various infectious agents, and men with symptoms of chronic inflammation in their noncancerous prostate tissue are about twice as likely as other men to develop advanced prostate canc More

  Inflammation is a physiological process begining with tissue damage in the presence of various infectious agents, and men with symptoms of chronic inflammation in their noncancerous prostate tissue are about twice as likely as other men to develop advanced prostate cancer. Considering the importance of inflammatory factors in the invasion and metastasis of prostate cancer cells, we decided to evaluate the effect of the anti-inflammatory drug M2000 on two inflammatory genes involved in the invasion of cancer cells. PC3 cells were first cultured using RPMI medium with 10% FBS and 1% PenStrep. The cells were then treated with M2000 at concentrations of 25 and 50 μg/ml and after 24 hours the expression of il-8 and NF-κB were examined using real-time RT-PCR. NF-κB was assessed using real-time RT-PCR. In this study, NF-κB gene expression decreased in all treatments in RT-PCR. While the il-8 decreases in expression at a concentration of 50 μg/ml. The results of the present study showed that M2000 can reduce the expression of il-8 and NF-κB genes involved in tumorigenesis and invasion of prostate cancer cells, which could pave the way for future studies to better control prostate cancer. Manuscript profile
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  16 - The effect of Tamsulosin with or without Tolterodine in treatment of patients with symptomatic BPH in Aria and 22 Bahman hospital in 2012
  Masoud Isapour Mahsa Abdollahian Moghadam
  Background: Although voiding symptoms are classically related to BPH, as many as 50% of patients can experience irritative symptoms. We compared efficacy of tamsulosin with or without tolterodine in the treatment of patients with symptomatic  BPH (benign prostatic More

  Background: Although voiding symptoms are classically related to BPH, as many as 50% of patients can experience irritative symptoms. We compared efficacy of tamsulosin with or without tolterodine in the treatment of patients with symptomatic  BPH (benign prostatic hyperplasia). Methods: seventy six patients who fulfilled the inclusion/ exclusion criteria were randomly divided into two groups of tamsulosin monotherapy (0/4 mg/day until 12 weeks) versus tamsulosin (0/4 mg/day until 12 weeks) and Tolterodine (2mg BID for 4 weeks) combination therapy. Therapeutic efficacy was analyzed using international prostate symptom score (IPSS) before and after 4 weeks of treatment. Results: Total IPSS was significantly improved in both groups. Although Combination therapy group showed better improvement in all Lower urinary tract symptoms, but the difference between 2 groups was significant considering complete emptying of bladder, voiding duration less than 2 hours and weak stream (P<0/05). Conclusion: Addition of Tolterodin to Tamsulosin is effective in improving lower urinary tract symptoms in patients with benign prostatic hyperplasia.   Manuscript profile