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Open Access Article
1 - ارزیابی فعالیت آنزیمهای شاخص آسیب قلبی پس از حذف پریکارد در خرگوش
O. Samiee Amlashi, , Gh. Abedi محمد Nasrollahzadeh Masouleh, , S. Hesarakiپریکاردیکتومی یک شیوه درمانی موثر برای برخی از بیماری­های قلبی است و استفاده از بیومارکرهای قلبی شیوه مهمی برای ارزیابی اثرات جراحی بر سلامت قلب هستند. در این راستا، ﻛـﺮاﺗﻴﻦﻛﻴﻨﺎز، آﺳﭙﺎرﺗﺎت­آﻣﻴﻨﻮﺗﺮاﻧﺴﻔﺮاز و ﻻﻛﺘـﺎت­دﻫﻴﺪروژﻧﺎز ﺑﻪ­ﻋﻨﻮان آﻧﺰﻳﻢ­ﻫﺎی وﻳﮋه آﺳ Moreپریکاردیکتومی یک شیوه درمانی موثر برای برخی از بیماری­های قلبی است و استفاده از بیومارکرهای قلبی شیوه مهمی برای ارزیابی اثرات جراحی بر سلامت قلب هستند. در این راستا، ﻛـﺮاﺗﻴﻦﻛﻴﻨﺎز، آﺳﭙﺎرﺗﺎت­آﻣﻴﻨﻮﺗﺮاﻧﺴﻔﺮاز و ﻻﻛﺘـﺎت­دﻫﻴﺪروژﻧﺎز ﺑﻪ­ﻋﻨﻮان آﻧﺰﻳﻢ­ﻫﺎی وﻳﮋه آﺳﻴﺐ قلبی از اﻫﻤﻴﺖ ﺧﺎﺻﻲ ﺑﺮﺧﻮردارند. هدف از این تحقیق بررسی فعالیت این آنزیم­ها بعد از پریکاردیکتومی در خرگوش­های سالم بود. تعداد 12 سر خرگوش سفید نیوزلندی نر هم­سن با وزن تقریبی 3/0±5/1 کیلوگرم به­ طور تصادفی به دو گروه شاهد و مورد تقسیم شدند. خرگوش­های گروه مورد، عمل پریکاردیکتومی و خرگوش­های گروه شاهد فقط تحت عمل توراکتومی قرار گرفتند. به­ منظور بررسی تغییرات آنزیم­های کراتین­کیناز، لاکتات­ دهیدروژناز، آسپارتات ­آمینوترانسفراز، نمونه­ گیری خون، از تمامی خرگوش­های جراحی شده در هفته ­های اول، دوم، سوم و چهارم بعد از عمل جراحی، انجام شد. براساس نتایج به­دست آماده، مقادیر کراتین­ کیناز در هفته ی اول اختلاف معنی ­داری نسبت به گروه شاهد داشت (05/0 > p). مقادیر کراتین­ کیناز گروه مورد در هفته اول در مقایسه با هفته ­های دوم، سوم و چهارم از نظر آماری افزایش معنی ­داری را نشان داد (05/0 > p). میزان لاکتات­ دهیدروژناز و آسپارتات­ آمینوترانسفراز گروه مورد در هفته­ دوم افزایش معنی ­داری داشتند (05/0 > p). مقادیر لاکتات ­دهیدروژناز و آسپارتات آمینوترانسفراز گروه مورد در هفته دوم در مقایسه با هفته ­های اول، سوم و چهارم اختلاف معنی­داری را نشان دادند (05/0 > p). به­طورکلی می­توان نتیجه گرفت که پریکاردیکتومی باعث افزایش موقت فعالیت آنزیم­های شاخص آسیب قلبی در هفته­ اول و دوم می­شود و بعد از پایان هفته دوم سطوح آنزیم­های کراتین­ کیناز، آسپارتات­آمینوترانسفراز و لاکتات ­دهیدروژناز به حد طبیعی خود برگشته و این افزایش مقادیر دائمی نیستند. Manuscript profile -
Open Access Article
2 - Diagnostic Value of Milk Enzymatic Markers Lactate Dehydrogenase and Alkaline Phosphatase for Subclinical Mastitis in Dairy Cattle
سعید Akbari Aliabad S. O. Ghasemian S. Z. PeighambarzadehMastitis could be diagnosed by diverse methods, including physical, clinical, and laboratory tests. The present study aimed to evaluate the accuracy of subclinical mastitis diagnosis using enzymatic diagnostic tests for milk lactate dehydrogenase (LDH) and alkaline phos MoreMastitis could be diagnosed by diverse methods, including physical, clinical, and laboratory tests. The present study aimed to evaluate the accuracy of subclinical mastitis diagnosis using enzymatic diagnostic tests for milk lactate dehydrogenase (LDH) and alkaline phosphatase (ALP). This study was performed on 90 Holstein cows selected randomly out of high- and low-yielding dairy cattle. According to the result of the California Mastitis Test, two milk samples were obtained from the intended udder quarters based on the principles of the National Mastitis Council. One specimen was used for microbial culture and the other one was utilized for somatic cell count (SCC), as well as milk LDH and ALP tests by diagnostic kits. Considering microbial culture as the gold standard for subclinical mastitis diagnosis, the sensitivity of SCC, ALP, and LDH tests was 87%, 59.3%, and 70.4%, respectively. Moreover, the specificity of these tests was 77.8%, 80.6%, and 77.8%, respectively. Our findings revealed a strong correlation between SCC, LDH, and ALP. It was observed that an increase in SCC was accompanied by augmented LDH response. According to the results of the present study, SCC was the most efficient test for diagnosing subclinical mastitis in dairy cows, followed by LDH and ALP. Manuscript profile -
Open Access Article
3 - The Effect of Selenium Supplementation on Serum Levels of Creatine Kinase, Lactate Dehydrogenase and Muscle Strength at Resting and after a Soccer Match in Young Soccer Players
M. Azimi F. MoradiIntroduction: Few researches have been carried out on the effect of selenium supplementation on muscle damage in athletes. The aim of this study is to investigate the effect of selenium supplementation on serum levels of creatine kinase (CK), lactate dehydrogenase (LDH) MoreIntroduction: Few researches have been carried out on the effect of selenium supplementation on muscle damage in athletes. The aim of this study is to investigate the effect of selenium supplementation on serum levels of creatine kinase (CK), lactate dehydrogenase (LDH) and muscle strength at resting and after a soccer match in young soccer players. Materials and Methods: The research method was quasi-experimental and 19 young soccer players were selected among soccer players in Saghez city and were randomly divided into two experimental (supplementation, n=10) and control (placebo, n=9) groups. The assessments were carried out in three stages (pre-supplementation, post-supplementation, and immediately after the match). General characteristics of subjects, muscle strength, and circulating levels of LDH and CK were measured. The supplementation period was one month (one 200 µg/ml selenium capsule daily). The placebo group also consumed a starch capsule each day. In order to analyze the data, two factor analysis of variance with repeated measures were used at the significant level of P<0.05. Results: Selenium supplementation has no effect on muscle strength (bench-press p=0.790, leg-press p=0.912) and levels of CK (p=0.051) and LDH (p=0.244) at rest and after the soccer match. Conclusion: Selenium supplementation for one month and a daily intake of 200 μg does not appear to have a beneficial or harmful effect on muscle strength and circulating levels of enzymes indicating muscle damage at rest and following a soccer match in young soccer players. More research is needed to reveal various aspects of the issue. Manuscript profile -
Open Access Article
4 - The response of oxidative stress indicators to grape seed extract supplementation and resistance training in bodybuilders
fariba joshaghani hamzeh rahmani amin yosefvandBlack grape seed extract (GSE) is one of the supplements that have strong antioxidant effects with flavonoid properties that can prevent or reduce cell damage. The purpose of this study was to investigate the response of oxidative stress indicators to grape seed extract MoreBlack grape seed extract (GSE) is one of the supplements that have strong antioxidant effects with flavonoid properties that can prevent or reduce cell damage. The purpose of this study was to investigate the response of oxidative stress indicators to grape seed extract supplementation and resistance training in bodybuilders. The methodology of the present research was conducted in the form of a semi-experimental design in two supplement and control groups with blood sampling on two occasions before and after taking the supplement in a double-blind manner. The statistical population of the present study consisted of 24 male bodybuilders with an age range of 15-19 years and they were divided into two groups: supplement and placebo. About 5 ml of blood was taken from the subjects in each of the fasting blood sampling sessions. Measurement of blood indices included antioxidant capacity (TAC), malondialdehyde (MDA), creatine kinase (CK), and lactate dehydrogenase (LDH). The normality of data distribution was done using ShapiroWilk tests. The findings of the research results indicate that performing bodybuilding resistance exercises caused a significant increase in cell damage indices (creatine kinase and lactate dehydrogenase) and malondialdehyde index and a significant decrease in total antioxidant capacity (P<0.05). Consumption of grape seed extract increased total antioxidant capacity. The present results show that performing bodybuilding activities increases the indicators of cell damage and oxidative stress, and the consumption of grape seed extract (100 mg per day) increases the total antioxidant capacity in male bodybuilders. Manuscript profile -
Open Access Article
5 - The effect of intraperitoneal injection of hydroalcoholic extract of flaxseed on biochemical, hematological and liver enzymes in rats
Atefeh PayezBackground: Medicinal plants have a wide range of applications in various fields, especially in the control of microbial infections, but the discussion of their possible toxicity has caused great concern in the use of these substances in various fields of industry and m MoreBackground: Medicinal plants have a wide range of applications in various fields, especially in the control of microbial infections, but the discussion of their possible toxicity has caused great concern in the use of these substances in various fields of industry and medicine. This study was performed to clarify some of these ambiguities and to investigate the effect of intraperitoneal injection of flaxseed extract on biochemical, hematological, and hepatic factors in rats.Materials and Methods: In this experimental study, 15 female spiral rats were randomly divided into 5 groups. The first group was the sham group, the second group was the control group, and the third to fifth groups as experimental groups with concentrations of 200, 400, and 600 mg/ml of flaxseed extract were treated, respectively. The mice were treated for 10 days, then on the eleventh day the mice were anesthetized with ether and blood samples were taken from their hearts. Then biochemical parameters, blood, and lactate dehydrogenase, and plasma levels of liver enzymes were evaluated.Results: The results of this study did not show much change in biochemical factors except cholesterol and triglycerides which showed a significant decrease in the extract-treated groups. Blood factors did not show significant changes but increased in high concentrations of liver enzymes.Conclusion: Intraperitoneal injection of flaxseed extract has no effect on blood factors and many biochemical factors in mice, but high concentrations of the extract can affect liver enzymes. Manuscript profile -
Open Access Article
6 - Antioxidant enzyme activities and fermentation metabolism in the root of three wheat promising lines under waterlogging stress
Freshteh alizade vaskasi hemmatollah Pirdashti Ali Cherati_Araei Sara SaadatmandWaterlogging stress has negative effects on the growth and yield of wheat plants, which recognizes the defense mechanism of the plant against waterlogging, can be valuable. In order to study the response of three wheat genotypes to different levels of waterlogging stres MoreWaterlogging stress has negative effects on the growth and yield of wheat plants, which recognizes the defense mechanism of the plant against waterlogging, can be valuable. In order to study the response of three wheat genotypes to different levels of waterlogging stress, a factorial experiment was conducted based on a completely randomized design with five replications in Gharakhil (Ghaemshahr) Agricultural Station. The objective of this study was to investigate the effects of waterlogging (0, 7, 14 and 21 day) at tillering (ZG21) and stem elongation (ZG31) stages on the growth, total chlorophyll, proline, malondialdehyde (MDA), H2O2, activity of antioxidant enzymes, and enzymes of fermentation pathway in root of three wheat promising lines (N-93-19, N-93-9 and N-92-9). The results showed that waterlogging stress in both growth stages reduced the total chlorophyll and growth of all three genotypes, however, the highest contents of total chlorophyll and shoot and root dry weight were observed in N-92-9 genotype. Increased waterlogging stress increased the contents of proline, MDA, H2O2, activity of superoxide dismutase, catalase, alcohol dehydrogenase and lactate dehydrogenase and decreased peroxidase activity of root of genotypes in both growth stages compared to control treatments. The results of this experiment that N-92-9 genotype had better response than other two genotypes in all studied traits under waterlogging conditions and was introduced as a flood tolerant genotype. Manuscript profile -
Open Access Article
7 - Comparison of Six Weeks of Weight Training with a Combination of Beta Hydroxy-Beta Methyl Butyrate and Creatine Supplement and Two Weeks of Non-Training on Creatine Kinase and Lactate Dehydrogenase in Male Powerlifting Athletes
Mojtaba Darvishkhadem Tahereh Bagherpour Nematollah NematiThe aim of this study was to compare six weeks of weight training with HMB and creatine supplementation and two weeks of non-training on creatine kinase and lactate dehydrogenase in male powerlifting athletes. Thirty-two male athletes aged 21 to 30 years were randomly d MoreThe aim of this study was to compare six weeks of weight training with HMB and creatine supplementation and two weeks of non-training on creatine kinase and lactate dehydrogenase in male powerlifting athletes. Thirty-two male athletes aged 21 to 30 years were randomly divided into four groups of 8 people creatine, HMB, HMB + Cr, and weight training. The training program was designed for 6 weeks and four sessions per week. HMB group 3 grams of supplement on training days and 1 gram on non-training days, creatine group on training days in 3 servings of 5 grams and 5 grams on non-training days, HMB + Cr group HMB supplement in 3 grams per day and Creatine was consumed in the same way as the creatine group. During the two weeks of training, the HMB group received 1 gr of the supplement daily, the creatine group received 5 gr of the supplement, and the HMB + Cr group received 1 gr of the HMB supplement daily and 5 grams of the creatine supplement. One-way analysis of variance with repeated measures was used to test the hypotheses. The test results showed that in the post-test between weight training groups and HMB (p = 0.000), creatine (p = 0.006) and HMB + Cr (p = 0.000) and in two weeks of inactivity, there was a significant difference in CK between the weight training group and HMB + Cr (p = 0.047). Also between weight training groups and HMB (p = 0.000), HMB + Cr (p = 0.000), creatine and HMB (p = 0.019), creatine and HMB + Cr (p = 0.001) in both Week of inactivity There was a significant difference in LDH between the weight training group and HMB + Cr (p = 0.004), creatine and HMB (p = 0.026), creatine and HMB + Cr (p = 0.001). It seems that 6 weeks of weight training with a combination of HMB and creatine supplement and 2 weeks of non-training can significantly reduce muscle injury indices in male powerlifting athletes. . Manuscript profile -
Open Access Article
8 - The Effect of Different Degrees of Dehydration on Plasma Creatine Kinase and Lactate Dehydrogenase Levels of Professional Wrestlers in Ahvaz
mehdi bostani reza sheikhDespite the proving of the negative effects of dehydration, many athletes use of this strategy yet. The aim of this study was to investigate the effect of dehydration with different degrees on serum levels of enzymes biomarker muscle damage in professional wrestlers in MoreDespite the proving of the negative effects of dehydration, many athletes use of this strategy yet. The aim of this study was to investigate the effect of dehydration with different degrees on serum levels of enzymes biomarker muscle damage in professional wrestlers in Ahvaz city. Among the professional wrestlers in Ahvaz city, after screening, 40 wrestlers were selected and randomly divided into three experimental groups and one control group. Experimental groups underwent dehydration at three levels of mild, moderate and severe. The plasma levels of Creatine Kinase (CK) and Lactate Dehydrogenase (LDH) were measured by enzyme kinetics method. for intragroup comparison and intergroup comparison, were used of the dependent t-test and analysis of variance (ANOVA) test respectively at a significance level of 0.05. The results showed that plasma CK levels increased significantly in the moderate and severe dehydration groups and LDH levels increased only in the severe dehydration group (P <0.05). Also, in the intergroup comparison of CK levels, it was observed that there was a significant difference between the severe dehydration group and other groups, as well as the moderate dehydration group and other groups (P <0.05). But no significant difference was observed between the moderate and severe dehydration groups (P <0.05). Also, the results of comparison between groups of LDH showed that only the difference between the severe dehydration group with other groups was significant (P <0.05) and the difference between the other groups was not significant (P <0.05). According to the results of this study, it is suggested that to prevent the possibility of muscle damage and consequently reduced the exercise performance, avoided the dehydration of more than 1% in one session and if necessary, dehydrate in more sessions and for longer. Manuscript profile -
Open Access Article
9 - تاثیر مکمل سازی اسید چرب امگا- 3 بر شاخص التهابی CRP و شاخصهای آسیب سلولی سرمی پس از یک جلسه تمرین مقاومتی در هندبالیس تهای مرد جوان
شهلا حجت سیروان آتشک محمد امین گلی -
Open Access Article
10 - Determination of the diagnostic value of milk lactate dehydrogenase and alkaline phosphatase in bovine subclinical mastitis
عباس Kalantari SH Safi عباس Rahimi ForoshaniCurrently somatic sell count(SCC) and bacterial culture is considered as the gold standard of detecting subclinical mastitis.however,the ebove-mentioned tests have a low diagnostic accuracy.therefore,for identification of infected animals,new biomarkers with high clinic MoreCurrently somatic sell count(SCC) and bacterial culture is considered as the gold standard of detecting subclinical mastitis.however,the ebove-mentioned tests have a low diagnostic accuracy.therefore,for identification of infected animals,new biomarkers with high clinical accuracy are needed.the objective of this study was to determine the diagnostic value of milk lactate dehydrogenase(LDH) and alkaline phophatase(ALP_ for the diagnisis of subclinical mastitis in dairy cows.the activities of these enzymes increase during mastitis,which make them to be the potential biomarkers for screening of mastitis.a total of 145 subclinical healthy cows were randomly selected .of these 77 cows were considered to be affected by sub clinical mastitis based on a SCC higher than 100*1000 cells/ml of milk and positive bacterial culture results of milk samples obtained from at least one of the quarters.enzymes activities were measured in blood serum and defatted milk(centrifuged at 5000-g for 15 min at 4)using commercial kits.diagnostic sensivity and specificity and cutoff points for each test were determined via receiver-operating characteristics curve.significant (p<0.001)increases in the mean and median activities of LDH and ALP were found in the milk samples collected from cows with subclinical mastitis.milk LDH had the most clinical accuracy with 94.8% sensitivity and 94.1% specificity at cutoff point of 109 U/L.the results of the present study showed that the measurement of LDH and ALP activities in milk samples could be used as reliable method for detection of bovine subclinical mastitis. Manuscript profile
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