فیتوفاگ

Open Access Article

1 - Survey on histopathological lesions of silver carp and grass carp to lernea cyprinacea infestion in some carp’s rearing farms in Shushtar
علیرضا گلچین‌منشادی، میلاد عامری، رضا صادقی‌لیمنجوب .

Lernaea cyprinacea is commonly called the ‘anchor worm’. Muscle necrosis, haemorrhaging, inﬂammation and suppuration are common. Acute pathology occasionally happens when several parasites settle close together. The necrosis of tissues can cause serious lesi More

Lernaea cyprinacea is commonly called the ‘anchor worm’. Muscle necrosis, haemorrhaging, inﬂammation and suppuration are common. Acute pathology occasionally happens when several parasites settle close together. The necrosis of tissues can cause serious lesions that can become secondarily infected by bacteria or fungi. Inﬂammation and then tissue growth around the site of entry encapsulate the parasite’s body.This survey in order to identify histopathologic effects of Lernea was performed on silver carp and grass carp. For this purpose 80 carp has been hunted from four ponds of shushtar city with random selection (20 fish from each pond). All fish hunted in summer season. Sampling was performed by cutted skin and muscle from parasite attachment and placed into 10% formalin buffer. Pathological slides were prepared with standard method, then analyzed under close microscopic observation. The results showed inflammation with cellular infiltration and giant cell penetration, skin’s hyperplasia, new fibroblasts formation, melanosis, muscular necrosis, hyperemia, vascularization and hemorrhage which showed defense response via blood cell’s defense and formation of healing tissue. Most of parasites were isolated from skin area meanwhile, the total percentage of infection to lernea was 30%. Manuscript profile

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2 - شناسایی سریع گونه‌های سودوموناس در ماهی فیتوفاگ (Hypophthalmichthys molitrix) پرورشی در استان گیلان
متین وظیفه دوست سمیه حقیقی کارسیدانی خسرو عیسی زاده محدث قاسمی محمد فائزی قاسمی

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Open Access Article

3 - The effect of nisin A and sodium benzoate on behavior of Listeria monosytogenes and some microbial and chemical parameters in silver carp (Hypophtalmichtys molitrix) fillet stored at 4˚C
رضا Safari M.R Saeidi Asl

   The effect of nisin A and sodium benzoate on Listeria monocytogenes, as well as some microbial (mesophilic, psychrotrophic and lactic acid bacteria) and chemical (peroxide and TVN) in silver carp (Hypophtalmichtys molitrix) fillet during storage period (0, More

   The effect of nisin A and sodium benzoate on Listeria monocytogenes, as well as some microbial (mesophilic, psychrotrophic and lactic acid bacteria) and chemical (peroxide and TVN) in silver carp (Hypophtalmichtys molitrix) fillet during storage period (0, 3, 6, 9 and 12 days) at 4˚C were evaluated. For this, Listeria monocytogenes (4 logCFU/g) was inoculated to the fillets and were dipped into 2% sodium benzoate solution for 15 min and left to stand for 10 min at 4˚C. Subsequently nisin A was added to the fillet (0/15 g/kg) and samples were kept at 4˚C while packaged in vacuum condition. The results showed that, application of nisin A and sodium benzoate decrease the number of Listeria monocytogenes from 4/12 to 3/66. However, in control groups the number of bacterium was increased from 4/43 to 5/14. Moreover, the number of mesophilic bacteria in treatment and control groups was increased from 4/39 to 6/79 and 4/48 to 7/93, respectively. The number of psychrotrophic bacteria in treatment and control groups was increased from 4/16 to 6/72 and 4/34 to 7/92, respectively. The similar result was achieved for lactic acid bacteria in which the number of these bacteria was increased from 2/74 to 4/08 and 2/9 to 4/78, respectively. Moreover, different peroxide value and TVN for treatment and control groups was achieved. In conclusion, application of nisin A and sodium benzoate showed different inhibitory effects on Listeria monocytogenes in culture media and silver carp. Manuscript profile

Open Access Article

4 - Effect of vacuum packaging on the shelf-life and quality of Hypophthalmichthys molitrix fillets during storage in refrigerator temperature
مهران Javaheri Baboli محمد Velayatzadeh مهدی Jagir اکبر Pashaei

   Vacuum packaging is one of the appropriate methods in packaging and storage of fish products. In this study, chemical changes including pH, thiobarbituric acid (TBA), total volatile basic nitrogen (TVN) and microbial counts such as coliform bacteria, fecal More

   Vacuum packaging is one of the appropriate methods in packaging and storage of fish products. In this study, chemical changes including pH, thiobarbituric acid (TBA), total volatile basic nitrogen (TVN) and microbial counts such as coliform bacteria, fecal coliform, salmonella and Escherichia coli in vacuum packed fillet of silver carp (Hypophthalmichthys molitrix) was performed during 30 days storage at 4 ± 1 °C. Fresh silver carp fish samples (ranged 900 to 1200 g) were obtained and transported to the laboratory. Data were analyzed using SPSS 18 software. Results showed that the highest levels of TVN and pH were determined as 63.33 ± 1.82 mg/100 g and 6.81 ± 0.04 in the day 30, respectively. According to the results, TBA, TVN and pH values were increased during 30 days storage. Moreover, total bacterial count increased during the 30 days of storage. Results revealed that coliform counts were less than 2 log cfu/g during the 30 days of storage. Increasing trend of faecal coliform at the end of storage period was estimated as 2.38±0.01 log cfu/g. Considering the microbial populations and the chemical spoilage indicators (TBA and TVN), the shelf life of 15 days was established for the vacuum packaged fillet stored at 4±1°C. Manuscript profile

Open Access Article

5 - Investigation of Cadmium on Some Biochemical Indices of the Farmed Silver Carp (Hypophthalmichthys molitrix)
Mahzad Shakoori S. Abdali S. Shokohi

The aim of this study was to evaluate the toxicity effect of Cadmium heavy metal on some serological parameters of Silver carp which have been in the vicinity of cadmium for a short term. In this study a total of 135 fish with mean weight of 50±10grand mean lengt More

The aim of this study was to evaluate the toxicity effect of Cadmium heavy metal on some serological parameters of Silver carp which have been in the vicinity of cadmium for a short term. In this study a total of 135 fish with mean weight of 50±10grand mean length of 13±1cm were placed in 9 aquariums of 90-liter capacity, in contact with 0.5 and 10 mg/L of CdCl2.Sampling of fishwas done randomly every 12 hours until 96 hours from caudal vein. The results showed that, the level of triglyceride in 24 and 48 hours decreased significantly, but showed no significant increase in 72 hours. The level of cholesterol increased significantly at 72 and 96 hours with increasing the concentration of cadmium (P<0/05). Glucose level increased significantly in 72 hours, but significantly decreased with increasing the concentration of cadmium in 72 and 96 hours (P<0/05). The level of total protein decreased significantly in 24 and 96 hours with increasing the concentration of cadmium, and in 72 hours increased significantly (P<0/05). The level of albumin increased significantly in 96 hours.  Based on these results, biochemical parameters are useful for monitoring of cadmium acute toxicity in Silver Carp. Manuscript profile

Open Access Article

6 - Investigation of Zinc on some biochemical indices of the farmed Silver carp (Hypophthalmichthys molitrix)
M. Shakoori S. Abdali H. Negarestan

  The aim of this study was to evaluate the toxicity effect of zinc heavy metal on some serological parameters on Silver carp in the vicinity with zinc in short term. In order to do this study a total of 135 fish with mean weight 50±10gr and mean length 13& More

  The aim of this study was to evaluate the toxicity effect of zinc heavy metal on some serological parameters on Silver carp in the vicinity with zinc in short term. In order to do this study a total of 135 fish with mean weight 50±10gr and mean length 13±1cm were located to adjacent to 0.5 and 10 mg/L of Zn (NO3)2 in 9 Aquarium with 90 liter of capacity.Sampling of fish  was done randomly each 12 hours until 96 hours from caudal vein. The result showed that the level of cholesterol in 10 mg/L treatments in 96 hours were significant difference with 5 mg/ L and control treatments (p<0/05). The results showed that the minimum amount of cholesterol in 10 mg/L treatment in 96 hours was (10.83±0.2 mg/ dL) and the maximum amount was found in 5 ppm treatment in 48 hours was (102.7±22.7 mg/dL). Mean triglyceride fluctuated, so that the maximum amount of  triglyceride was found in 10 mg/L treatment in 12 hours (93±14) mg/dL) and the minimum in 96 hours in 5 mg/L treatment was (24.4±2 mg dl). By increasing the concentration of zinc. albumin level were increased significantly. The minimum amount was found in control treatment which was (0.73±0.14 g/dL) and the maximum level in 5 m/g/L treatment at 96 hours was (9.03±0.6 g/dL). Total protein levels were decreased significantly (p<0.05). The minimum amount of total proteins in 5 mg/L treatment at 96 hours was (0/043±0/003) g/dL, and the maximum amount was at 12 hours in 10 mg/L treatment at (3/53±0/3)g/dL. Based on results, Hematological and biochemical parameters are useful for monitoring of Zinc acute toxicity in Silver carp. Manuscript profile

Open Access Article

7 - Identification of fatty acids composition in fresh and frozen silver carp (Hypophthalmichthys molitrix) for determination of its shelf life at -18ْc
A. R. Najafi N. Beheshtiseresht

In this work, after extraction and methylation of the lipids form silver carp tissues in fresh and frozen stages and their changes during 6 months in -18°C, identification of fatty acids of the samples were done by gas chromatography (Shymatsv HSSA4). Results of thi More

In this work, after extraction and methylation of the lipids form silver carp tissues in fresh and frozen stages and their changes during 6 months in -18°C, identification of fatty acids of the samples were done by gas chromatography (Shymatsv HSSA4). Results of this investigation showed that amounts of unsaturated and saturated fatty acids in fresh samples were 70/95% and 28/95% respectively. In fresh tissues the amount of oleic acid was 30/16%, and Iino leice amount was 11/44%. Alpfa – lino lenice acide was 4/41%, Gcosapanethanoic was 6/42% and docosa hexanoic was 6/15%. These were the most important poly-saturated fatty acids. But after six months of freezing at -18° c the amounts of these fatty acids decreased to 10/02%, 3/18%, 2/97%, 1/05% and 1/02% respectively. Results showed a decrease in freezing condition of fatty acids. Based on these results, the best storage for frozen fish in -18°C is four months. These results were subjected to tests of Tukey and Analysis of Variance and were significant at the level of 95%.   Manuscript profile

Open Access Article

8 - Investigation effect of Zinc on some hematological and immunological indices of the farmed Silver Carp (Hypophthalmichthys molitrix)
M. Shakoori S. Abdali H. Negarestan A. Hallajian

This study aims to elucidate the toxic impact of zinc on hematological indices of the farmed Silver Carp (Hypophthalmichthys molitrix). In this study, after adaptation 135 fish specimens with the length of 13±1 cm and the weight of 50±10 gr were put in one More

This study aims to elucidate the toxic impact of zinc on hematological indices of the farmed Silver Carp (Hypophthalmichthys molitrix). In this study, after adaptation 135 fish specimens with the length of 13±1 cm and the weight of 50±10 gr were put in one control treatment and 2 experimental treatments contained of 5 and 10 mg/L (Zn (no) 3)2 in 90 liter aquariums. Blood samples were taken from the caudal vein after 12, 24, 48, 72 and 96 hours. Results showed that, hematocrite (Hct), hemoglobin levels (Hb), mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH) increased significantly (p<0/05). The number of White Blood Cell (WBC) on 5 mg/L and 10 mg/lit treatments until 72 hours decreased significantly but after 96 hours it increased significantly (P<0/05). The number of Red Blood Cells (RBC) decreased significantly until 96 hours (P<0/05). According to the results, blood parameters were sensitive factors in monitoring the toxicity and the stress caused by zinc particularly in acute concentrations. Manuscript profile

Open Access Article

9 - Effect of Lead on Some Biochemical Indices of Farmed Silver Carp (Hypophthalmichthys molitrix)
M. Shakoori A. Abdali

The aim of this study was to evaluate the toxicity effect of lead heavy metal on some serological parameters on Silver Carp in the vicinity with lead for a short term. In order to do this study a total of 135 fish with mean weight of 50±10 gand mean length of 13& More

The aim of this study was to evaluate the toxicity effect of lead heavy metal on some serological parameters on Silver Carp in the vicinity with lead for a short term. In order to do this study a total of 135 fish with mean weight of 50±10 gand mean length of 13±1 cm were placed in adjacent to 0, 5 and 10 mg/l of Pb (NO3 )2 in 9 aquariums of 90 liter capacity.Sampling was done randomly each 12 hours for 96 hours from caudal vein of the fishes. The results showed that there was a significant difference in the amount of hemoglobin in 24 hours, between the control and treatments. The level of glucose was increased significantly by increasing the concentration of lead and passing of the time. The levels of triglyceride and albumin did not change significantly (P>0/05). The level of cholesterol increased significantly until 72 hours and after that decreased significantly. Amount of total protein increased significantly by increasing the concentration of lead and time. Based on the results, biochemical parameters are useful for monitoring of lead acute toxicity in Silver Carp. Manuscript profile

Open Access Article

10 - The effect of cadmium on some immunological indices in Silver carp (Hypophthalmichthys molitrix)
سورنا ابدالی S. Abdali A. Yousefi Jourdehi M. Yarmohammadi

In this study, 135 farmed Silver Carp fish with a mean weight of 50.6 ± 8 .6 gramsand a mean length of 16.2±3 cm , were exposed to concentrations of 0, 5 and 10mg/L of cadmium chloride (Cd(Cl)2) in 100 L aquariums for 96 hours. Eachtreatment had 3 replicat More

In this study, 135 farmed Silver Carp fish with a mean weight of 50.6 ± 8 .6 gramsand a mean length of 16.2±3 cm , were exposed to concentrations of 0, 5 and 10mg/L of cadmium chloride (Cd(Cl)2) in 100 L aquariums for 96 hours. Eachtreatment had 3 replicates and each replicate consisted of 15 fish. Blood from thefishes was taken every 24 hours. The number of white blood cells decreased in 72hours, but they increased in 96 hours (P<0.05). The number of neutrophils decreasedwith time. However, the number of eosinophils increased at concentration of 10 mgin 96 hours and reached to the maximum. The number of monocytes showedirregular fluctuations. Thus, increase of the heavy metal cadmium level in wateraffected immune system in Silver Carp negatively. Manuscript profile

Open Access Article

11 - اثرات نیترات روی Zn(NO3)2 بر بافت کبد ماهی فیتوفاگ (Hypophthalmichthys molitrix)
علی حلاجیان ایوب یوسفی جوردهی رضوان اله کاظمی سورنا عبدالی مهزاد شکوری

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12 - تاثیر شوری بر رشد و بازماندگی ماهی فیتوفاگ انگشت قد (Hypophthalmichthys molitrix)
زهرا محمدی مکوندی پریتا کوچنین حسین پاشا زانوسی

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13 - Effect of Salvia officinalis Extract with Vacuum Packaging on Physicochemical Properties of Hypophthalmichthys Molitrix Fish During Refrigeration Storage
Fateme Kolaie moakhar Mehran Moslemi Rozbeh Abedi

In this project, the effect of salvia officinalis extract coating in three treatments (at concentration of 1, 2, and 3%, respectively) was investigated on chemical and microbiological characteristics of fillets prepared from hypophthalmichthys molitrix during the 14-day More

In this project, the effect of salvia officinalis extract coating in three treatments (at concentration of 1, 2, and 3%, respectively) was investigated on chemical and microbiological characteristics of fillets prepared from hypophthalmichthys molitrix during the 14-day refrigeration storage (4 °C) period. On days 1, 4, 7, 10 and 14 of storage, the treatments were chemically analyzed in terms of thiobarbituric acid (TBA) and total volatile base nitrogen (TVB-N), microbial load (total bacterial load) and moisture content, acidity (PH), and water holding capacity (WHC). Based on the obtained results, the amount of TVB-N in all treatments was significantly reduced compared to control treatment (p < 0.05). The increasing trend of bioaburic acid during storage period in all treatments was significantly lower than the control treatment. Considering total bacterial load, all of the coated specimens had significantly lower bacterial load compared to the control group except for the first day. Based on the evaluation results of treatments moisture during the storage period, there was a significant difference in all indices except on day 14. There was also no significant difference between samples with regard to samples WHC. Considering pH, a significant difference was observed in indices on different days between samples except on days 4 and 14. In relation to pressurized water, the obtained data from treatments showed no significant difference except on day 4. Considering that treatment 3 (treatment coated with 3% salvia extract) showed more advantages in most indices compared to other coated treatments, the above mentioned coating was therefore recognized as the best treatment in this study and its application is recommended for effective preservation of the chemical and microbial properties of food products during the storage period. Manuscript profile

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