This study aimed to investigate the antibacterial effect of propolis alcoholic extract on the number of bovine mastitis isolates. For this purpose, agar disk diffusion method for determination of bacterial sensitivity and dilution method for determination o More
This study aimed to investigate the antibacterial effect of propolis alcoholic extract on the number of bovine mastitis isolates. For this purpose, agar disk diffusion method for determination of bacterial sensitivity and dilution method for determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) was used separately. The results of bacterial sensitivity test in the case of the standard antibiotics of tetracycline, amikacine and also disks impregnated with alcoholic extracts of propolis showed that the diameter of inhibition zone created by the mentioned combination about all of tested isolates was far larger and this difference about Escherichia coli and Klebsiella pneumonia isolates, was significant (p<0.05). Also the results of MIC and MBC tests indicated that the inhibition of bacterial growth by alcoholic extract of propolis had a direct relationship with the amount of available propolis in the dilution and increasing of propolis in each dilution, reduced the number of cultured bacterial colonies and no bacterial growth was observed in the dilution equivalent to MBC of propolis. Therefore, it seems that constitutes of this natural compound could be used as effective antibacterial agents against a wide range of bacteria causing bovine mastitis instead of using synthetic antibiotics.
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Mastitis is inflammation which causes physical and chemical changes in milk and pathological changes in mammary gland. The aims of present study were identification of bovine mastitis causative agents in Isfahan and survey of bee Propolis and Pollen antibacterial effect More
Mastitis is inflammation which causes physical and chemical changes in milk and pathological changes in mammary gland. The aims of present study were identification of bovine mastitis causative agents in Isfahan and survey of bee Propolis and Pollen antibacterial effect on the isolated bacteria. Milk samples were aseptically obtained from 10 mastitic cattle. The isolated bacteria were detected based on macroscopic, microscopic and biochemical characteristics. Ethanol and acetone extracts were prepared from Propolis and Pollen by soaking and the antibacterial effects of them determined using well plate and microdilution methods. The isolated bacteria included Streptococcus agalactiae, Staphylococcus aureus, Corynebacterium sp., Listeria sp., Pseudomonas aeruginosa and Escherichia coli. Well plate method showed that Propolis and Polen extracts were effective on Gram-positive bacteria but had no effect on Gram-negative ones. The mean of results showed maximum effects of Pollen acetone extract on Streptococcus agalactiae (concentration of 12.5 mg/ml) and Pollen ethanol extract on Corynebacterium (concentration of 12.5 mg/ml). Acetone extract of Propolis showed maximum effect on Staphylococcus aureus (concentration of 25 mg/ml) and Listeria sp. (concentration of 12.5 mg/ml). The results were confirmed by minimum bactericidal concentration analysis. Extracts of propolis and pollen showed powerful effects on Gram-positive isolates and this effect was more detected by acetone extracts.
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این آزمایش در کالج دانشگاه کشاورزی صلاح الدین اربیل برای بررسی اثر مکمل کردن آب با برهموم روی عملکرد و بعضی از خصوصیات فیزیولوژیکی و ایمونولوژیکی جوجه بلدرچین‌ها تا سن 42 روزگی انجام شد. تعداد 450 جوجه بلدرچین تعیین جنسیت نشده یکروزه به طور تصادفی به 5 گروه تیماری اختص More
این آزمایش در کالج دانشگاه کشاورزی صلاح الدین اربیل برای بررسی اثر مکمل کردن آب با برهموم روی عملکرد و بعضی از خصوصیات فیزیولوژیکی و ایمونولوژیکی جوجه بلدرچین‌ها تا سن 42 روزگی انجام شد. تعداد 450 جوجه بلدرچین تعیین جنسیت نشده یکروزه به طور تصادفی به 5 گروه تیماری اختصاص یافته‌اند. جوجه بلدرچین‌ها در هر گروه تیماری در 3 تکرار (30 جوجه برای هر تکرار) تقسیم شدند. پنج تیمار جیره‌ای عبارت بودند از: (T1 شاهد، (T2100 میلیگرم برهموم در لیتر، (T3 200 میلیگرم برهموم در لیتر، (T4 300 میلیگرم برهموم در لیتر و (T5 400 میلیگرم برهموم در لیتر. وزن بدن ،افزایش وزن، مصرف خوراک،ضریب تبدیل خوراک، درصد تلفات و تولید روزانه تخم‌مرغ اندازه‌گیری شدند. در پایان آزمایش همه جوجه‌ها کشتار و شاخص تولید، درصد لاشه و درصد قطعات لاشه اندازه‌گیری شد. نتایج نشان داد که مکمل کردن آب با سطوح مختلف برهموم به طور معنی‌داری (05/0P<) وزن بدن ، وزن لاشه و اوزان ران،پشت و بال را در مقایسه با گروه شاهد افزایش داد. تیمار 300 میلیگرم برهموم در لیتر برای عملکرد‌های تولیدی تیمار بهتری در مقایسه با سایر تیمارها بود.
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