In vitro evaluation of antibacterial properties of propolis alcoholic extract on bovine mastitis isolates
Subject Areas : Veterinary Clinical Pathologyyounes anzabi 1 , sohayb shaghaghi 2
1 - استادیار گروه پاتوبیولوژی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران.
2 - دانشجوی دکترای دانشکده دامپزشکی، واحد تبریز، دانشگاه آزاد اسلامی، تبریز، ایران.
Keywords: Propolis, antibacterial effect, Alcoholic extracts, Isolates of mastitis,
Abstract :
This study aimed to investigate the antibacterial effect of propolis alcoholic extract on the number of bovine mastitis isolates. For this purpose, agar disk diffusion method for determination of bacterial sensitivity and dilution method for determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) was used separately. The results of bacterial sensitivity test in the case of the standard antibiotics of tetracycline, amikacine and also disks impregnated with alcoholic extracts of propolis showed that the diameter of inhibition zone created by the mentioned combination about all of tested isolates was far larger and this difference about Escherichia coli and Klebsiella pneumonia isolates, was significant (p<0.05). Also the results of MIC and MBC tests indicated that the inhibition of bacterial growth by alcoholic extract of propolis had a direct relationship with the amount of available propolis in the dilution and increasing of propolis in each dilution, reduced the number of cultured bacterial colonies and no bacterial growth was observed in the dilution equivalent to MBC of propolis. Therefore, it seems that constitutes of this natural compound could be used as effective antibacterial agents against a wide range of bacteria causing bovine mastitis instead of using synthetic antibiotics.
طباطبائی، ع.ح. و فیروزی، ر. (1380). بیماریهای باکتریائی دام. انتشارات دانشگاه تهران، صفحات: 24-20، 43-37 و 227-223.
• رضویان، ح.، خزاعی، ص.، کاظمی، ش. و سیدی، س.م. (1391). برهموم زنبورعسل و کاربرد آن برای سلامت دهان و دندان. مجله دانشکده دندان پزشکی اصفهان، دوره 8، شماره 5، صفحات: 11-1.
• سیدی، س.م و فرشینه عدل، م.ب. (1389). برهموم: داروئی طبیعی از کندوی زنبورعسل. ترجمه نشر نصوح اصفهان، ایران، چاپ سوم، صفحات: 148-107.
• مرادی، م. (1388). بررسی اثر ضدباکتریائی برهموم زنبورعسل روی باکتری Paenibacillus larvae عامل بیماری لوک آمریکائی زنبورعسل. نشریه دامپزشکی (پژوهش و سازندگی)، شماره 83، صفحات: 62-57.
• هاتفی، م.، محرابیان، ص.، نوحی، ا. و رفیعی طباطبایی، ر. (1387 ). بررسی خواص ضد جهشزایی عصاره الکلی برهموم به وسیله سالمونلا تیفیموریوم و میکروزوم. مجله علمی پژوهشی دانشگاه علوم پزشکی اراک، سال 11، شماره 2، صفحات: 110-102.
• Alippi, A.M., Albo, G.N., Reynaldi, F.J. and De Giusti, M.R. (2005). In vitro and in vivo susceptibility of the honeybee bacterial pathogen Paenibacillus larvae subsp. Larvae to the antibiotic Tylosin. Veterinary Microbiology, 109(12): 47-55.
•Almas, K., Mahmoud, A. and Dahlan, A. (2001). A comparative study of propolis and saline application on human dentin. A SEM study. Indian Journal of Dental Research, 12(1): 21-27.
• Anzabi, Y. and Khaki, A. (2015). Antibacterial Effects of the Essential Oils and Ethanol Extracts of the Native Plants; Ziziphora Clinopodioides on 3 Species of Urinary Tract Isolated Bacteria in Rats’ Experimental Model. Medical Journal of Tabriz University of Medical Sciences and Health Services, 37(3): 18-25.
•Babayi, L., Kolo, J.I. and Ijah, U.J. (2004). The antimicrobial activities of methanolic extracts of Eucalyptus camaldulensis and Terminalia catappa against some pathogenic microorganisms. Biochemistry, 16(2): 106-110.
•Banskota, A.H., Tezuka, Y.T. and Kadota, S. (2001) Recent-progress-in pharmacological research of propolis. Phytotherapy Research, 15(7): 561-571.
•Borrelli, F., Maffia, P., Pinto, L., Ianaro, A., Russo, A. and Capasso, F. (2002). Phytochemical compounds involved in the anti-inflammatory effect of propolis extract. Fitoterapia, 73(Suppl 1): S53-S63.
•Brumfitt, W., Hamilton Miller, J.M. and Franklin, I. (1990). Antibiotic activity of natural products: propolis. Microbios, 62(250): 19- 22.
•Chena, Ch.R., Shena, C.T., Wu, J.J., Yangb, H.L., Hsu, S.L. and Chang, C.M.J. (2009). Precipitation of sub-micron particles of 3, 5-diprenyl- 4hydroxycinnamic acid in Brazilian propolis from supercritical carbon dioxide anti-solvent solutions. The Journal of Supercritical Fluids, 50(2): 176-182.
• Isla, M.I. ( 2005). Study on propolis quality from Argentina. Biocell, 29(1): 60.
• Najafi, M.F., Vhedi, F., Seyyedin, M., Jomehzadeh, H.R. and Bozary, K. (2007). Effect of the water extracts of propolis on stimulation and inhibition of different celss. Cytotechnology, 54: 49-56.
•Marcucci, M.C. (1995). Propolis: Chemical composition, biological propertis and therapeutic activity. Apidologie, 26: 83-99.
• Meresta, T. and Meresta, L. (1988). Sensivity of Bacillus larvae to propolis extract in vitro. Medycyna Veterynaryina, 44(3): 169-170.
• Park, Y.K., Koo, M.H., Abreu, J.A., Ikegaki, M., Cury, J.A. and Rosalen, P.L. (1998). Antimicrobal Activity of propolis on oral microorganisms. Current Microbiology, 3(1): 24-28.
• Mohan Nair, M.K., Vasudevan, P. and Venkitanarayanan, K. (2005). Antibacterial effect of black seed oils on Listeria monocytogenes. Journal of Food Control, 16(5): 395-398.
•Kim, K.T., Yeo, E.J., Han, Y.S., Nah, S.Y. and Paik, H.D. (2005). Antimicrobial, anti-inflammatory, and anti-oxidative effect of water and ethanol-extracted Brazilian propolis. Food Science and Biotechnology, 14:474-478.
• Tosi, E.A., Re, E., Ortega, M.E. and Cazzoli, A.F. (2007). Food preservative based on propolis: bacteriostatic activity of propolis polyphenols and flavonoids up on Escherichia coli. Food Chemistry, 104(3): 1025-1029.
• Khalil, M.L. (2006). Biological activity of bee propolis in health and disease. Asian Pacific Journal of Cancer Prevention, 7(1): 22-31.
• Velazquez, C., Narvarro, M., Acosta, A., Angulo, A., Dominguez, Z., Robles, R., et al. (2007). Antibacterial and free-radical scavenging activities of sonoran propolis. Journal of Applied Microbiology, 103(5): 1747-1756.
• Park, Y.K., Alencar, S.M. and Aguiar, C.L.(2002). Botanical origin and chemical composition of Brazilian propolis. Journal of Agricultural and Food Chemistry, 50(9): 2502-2506.
•Seidel, V., Peyfoon, E., Watson, D.G. and Fearnley, J. (2008). Comparative study of the antibacterial activity of propolis from different geographical and climatic zones. Phytotherapy Research, 22(9): 1256-1263.
• Orsi, R., Sforcin, J., Rall, V., Funari, S., Barbosa, L. and Fernandes, J. (2005). Susceptibility profile of Salmonella against the antibacterial activity of propolis produced in two regions of Brazil. Journal of Venomous Animals and Toxins Including Tropical Diseases, 11(2): 109.
• Kujumgiev, A., Tsvetkova, I., Serkedjieva, Y., Bankova, V., Christov, R. and Popov, S. (1999). Antibacterial, antifungal and antiviral activity of propolis of different geographic origin. Journal of Ethnopharmacology, 64(3): 235-240.
• Viuda Martos, M., Ruiz Navajas, Y., Fernandez Lopez, J. and Perez Alvarez, J.A. (2008). Functional properties of honey, propolis, and royal jelly. Journal of Food Science, 73(9): 117-124.
• Sforcin, J.M. and Bankova, V. (2011). Propolis: is there a potential for the development of new drugs? Journal of Ethnopharmacology, 133(2): 253-260.
• Schaechter, M., Medoff, G. and Fchlessinger, D. (1989). Mechanisms of Microbial Disease. International Edition (Williams and Wilkins): 17-50.
•Moreno, S., Scheyer, T., Romano, C.S. and Vojnov, A.A. (2006). Antioxidant and antimicrobial activities of rosemary extracts linked to their polyphenol composition. Free Radical Research, 40(2): 223-231.
• Singh, A., Singh, R.K., Bhunia, A.K. and Singh, N. (2003). Efficacy of plant essential oils as antimicrobial agents against Listeria monocytogenes in hotdogs. Food Science & Technology, 36(8):787-794.