List of Articles β-glucanase

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  1 - Construction of glucanase gene of Bacillus subtilis in Escherichia coli
  Marzieh Shavali Abbas Doosti
  Background & Objectives: The grains are considered as the main food for poultries. Grains contain significant content of non- soluble starch polysaccharides (NSP). NSPs cause negative impact and incompatibility in birds. Digestion of NSP contents by β-glucanase More

  Background & Objectives: The grains are considered as the main food for poultries. Grains contain significant content of non- soluble starch polysaccharides (NSP). NSPs cause negative impact and incompatibility in birds. Digestion of NSP contents by β-glucanase enzyme supplement reduces the viscosity of polysaccharides in the digestive tracts, and increases their intestinal absorption. The aim of this study was to construction of glucanase gene of Bacillus subtilis in Escherichia coli. Materials & Methods: In this experimental study, the glucanase gene of B. subtilis was amplified using specific primers and polymerase chain reaction. After purification of the bands, Bgl gene was cloned by T/A cloning technique in pGEM vector, and was transformed in E. coli. Afterward, the pGEM-bgl was transferred into E. coli Top-10 strain. The recombinant vectors were then transformed into E. coli competent cells, and the recombinants were plated on LB agar containing 100 µg/ml ampicillin. The recombinant plasmid transformed to E. coli Top10 cell and the colonies carrying plasmid were selected by PCR. The presence of glucanase construction was confirmed by enzymatic digestion. Results: The results showed that the glucanase gene was successfully cloned in E. coli. The results of cloning of 767 bp glucanase gene was confirmed by PCR assay. Conclusion: The construction of  B. subtilils glucanase gene and cloning of this gene in E. coli is a strong alternative for the production of  probiotic used for poultry.   Manuscript profile
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  2 - Growth Performance and Energy Utilization of broilers fed High and Low Metabolizable Energy Diets Supplemented with Multi-Enzyme
  S.A.  Moftakharzadeh H.  Janmohammadi Samira Kian S.  Sadeghi S.  Yousefi
  This experiment was determined to investigate the effects of exogenous-enzyme addition to the high and low levels of metabolizable energy (ME), on performance, energy utilization and body composition of broiler chickens fed corn-soybean meal diets from 0 to 21 days of a More

  This experiment was determined to investigate the effects of exogenous-enzyme addition to the high and low levels of metabolizable energy (ME), on performance, energy utilization and body composition of broiler chickens fed corn-soybean meal diets from 0 to 21 days of age. 240 one-d-old male Ross 308 broil-ers were used in a completely randomized 2 × 2 factorial design, with six replicate cages per treatment. Two metabolizable energy level: 3150 or 2750 kcal/kg and two enzyme (with xylanase beta-glucanase, and phy-tase activity) level: 0 and 200 mg/kg dry matter (DM) were used. There was a main effect of energy (P<0.05) on feed intake of broilers and feeding 2750 kcal/kg ME diet increased feed intake (FI) of broilers in the whole period. The addition of enzyme to 2750 kcal/kg ME diet significantly (P<0.05) improved the average daily gain (ADG) and feed conversion ratio (FCR) of broiler chickens on 0-21 days of age (P<0.05). While net energy for production (NEp), NE, ME, organic matter, and dry matter digestibility were improved (P<0.05) by supplementing both energy level diets with enzyme at 21 d, only addition of enzyme to 2750 kcal/kg ME diet reduced heat production (HP) of broilers in the total trial (P<0.05). On day 21, the amount of NE improvement by enzyme supplementation was greater than ME for 2750 and 3150 kcal/kg, respectively. This study showed that NE is a more sensitive energy utilization measure than ME for evaluating the response of broilers to enzyme supplementation, and the energy retained as fat and protein in the body carcass was higher for the birds fed lower energy diet supplemented with enzyme. Manuscript profile