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      • Open Access Article

        1 - Phylogeny and genetic diversity of Indian crested porcupine (Hystrix indica) based on mtDNA cytochrome b in Fars province
        AliReza Sahraeian Jahromi Jalil Imani Harsini Hamidreza rezaei
        Background and Objective: In recent years, Hystrix indica as the biggest rodent in Iran has been in conflict with humans regarding the destruction of gardens and agricultural products, and the population of this species has been decreased due to illegal hunting and the More
        Background and Objective: In recent years, Hystrix indica as the biggest rodent in Iran has been in conflict with humans regarding the destruction of gardens and agricultural products, and the population of this species has been decreased due to illegal hunting and the use of pesticides and chemical poisons. Meanwhile there is not effective information which provide the possibility of developing a management and conservation plan for this species, so the aim of this study is to investigate the structure and genetic diversity of Hystrix indica in Fars province habitats. Material and Methodology: 15 quill and tissue samples of this species were collected in the Fars province habitats. After DNA extraction, mitochondrial cytochrome b gene was amplified in 13 samples by polymerase chain reaction and 912 nucleotide pairs of this gene were sequenced and edited for each sample. Findings: Based on the Maximum Likelihood Estimation of Phylogenetic Tree, all the studied samples are related to the same clade and belong to Hystrix indica species. Among the studied samples, only two different haplotypes were identified and the haplotype diversity was equal to 0.282 and the nucleotide diversity was estimated to be 0.00029. Discussion and Conclusion: the results showed that haplotype diversity of Hystrix indica is low in Fars province, so it is suggested to avoid further environmental crises like disruption of its food chain, while conducting demographic studies in order to develop a conservation and management plan for this species. Manuscript profile
      • Open Access Article

        2 - Evaluation of Turkish durum wheat (Triticum turgidum var. durum) genotypes based on quantitative traits and shoot zinc accumulation under zinc-deficient calcareous soil
        Ezatollah Esfandiari Majid Abdoli Behzad Sadeghzadeh Seyed-Bahman Mousavi
      • Open Access Article

        3 - Genetic evaluation of physiological Traits related to drought tolerance in some bread wheat genotypes under rain-fed conditions
        zahra morovati Ezatollah Farshadsfar Mohammad Hosein Romena
        Abstract: In order to investigate phenotypic and genotypic correlations between physiological characteristics related to drought tolerance and genetic variation and estimating the genetic parameters of these indices in rain-fed condition, 19 genotypes of bread wheat wer More
        Abstract: In order to investigate phenotypic and genotypic correlations between physiological characteristics related to drought tolerance and genetic variation and estimating the genetic parameters of these indices in rain-fed condition, 19 genotypes of bread wheat were cultivated in a randomized complete block design with three replications in the research field of Agricultural Research Campus Natural Resources of Razi University of Kermanshah, Iran during 2014-15. analysis of variance (ANOVA) showed significant differences for the grain yield (YS), the cell membrane stability )CMS), the relative water loss )RWL(, the leaf water loss (LWL), the relative water protective (RWP), the water use efficiency )WUE( and the leaf greenish value )LGV( under rain-fed condition. The polygon view of GT biplot showed that genotype no. 12 (wc-4931) was the best genotype compared to other genotypes due to YS, WUE, ELWR and LGV. A high positive genetic and phenotypic relation was observed between WUE and Ys, FV/FM and LWL and, ELWR and LGV. In other hand, High heritability and genetic gain were observed for YS, CMS and WUE that reflect the additive gene action. Accordingly, due to the high propotion of additive effects in CMS, YS and WUE, it was suggested that the evaluation of genotypes must be done at erarly generarion of inbreeding. Manuscript profile
      • Open Access Article

        4 - Evaluation of yield and yield components of some of faba feen (Vicia faba L.) genotypes at different sowing dates in Guilan province
        Peyman Sharifi
        The present experiment was carried out to study the effect of planting date on yield and yield componts of some faba bean genotypes in a split plot based on Complete Randomized Block Design with three replicates in Guilan province during 2014-15 growing season. The main More
        The present experiment was carried out to study the effect of planting date on yield and yield componts of some faba bean genotypes in a split plot based on Complete Randomized Block Design with three replicates in Guilan province during 2014-15 growing season. The main factor was four planting dates (20 November, 19 December, 5 January and 1 March) and the split factor was six faba bean genotypes (Guilan landrace, Borojerd landrace, France, Barrakat, FILIP3 and FILIP5). The effect of planting date was significant on all of the studied traits. The effect of genotype was also significant on all traits exept of days to maturity and relative water content. Interactions of two factors were significant on seed yield, number of days to emergence, hundred seed weight, number of seeds per pod and number of pods per plant. The highest ripening time of seeds (175.22 days) was observed in the first planting date and this time in the last planting date was reduced by 42%. Due to the significant interaction of genotype by sowing date, the simple effect of genotype was investigated at each level of sowing date and it was shown that in all four sowing dates, Gilan landrace had the highest grain yield with 3208.04, 1858, 110.8, 605.2 kg ha-1, respectively. Delay in planting incrased the seed nitrogen content by 22%. According to the results of present study and due to climatic ondition, early November is considered as an appropriate time for planting faba beans in the studied area. Manuscript profile
      • Open Access Article

        5 - The study of MHC-DRB3 gene Exon region polymorphisms by PCR-RFLP in some breeds of horses in Iran
        Mohammad Tavakoli Alireza Noshary Behzad Hemmati
        The major histocompatibility complex (MHC) genes are very important in horse breeding according to relationship with immunity response and resistance or susceptibility to disease. The aim of this research is study of polymorphism possible in exon gene region and populat More
        The major histocompatibility complex (MHC) genes are very important in horse breeding according to relationship with immunity response and resistance or susceptibility to disease. The aim of this research is study of polymorphism possible in exon gene region and population genetics. To do this, 120 horses were selected randomly from Thoroughbred, Oldenburg, Caspian pony and Arab breeds. Genomic DNA was extracted via blood samples and polymerase chain reaction was done for a 309 bp MHC gene region. The PCR products were digested by RsaI restriction endonuclease for genotyping determination. Results show that there were three allele of A, B and C and six types of genotype of AA, AB, AC, BB, BC and CC with 0.3208, 0.3208 and 0.3584 allele frequency and 0.05, 0.25, 0.2917, 0.1333, 0.125 and 0.15 genotype frequency in all studied samples respectively. The most and least allele frequency in Thoroughbred, Oldenburg, Caspian pony and Arab are related to B, A, A, C and A, B, B, A alleles respectively. The Hardy-Weinberg equilibrium study by X2 and G2 tests shows the equilibrium situation in Oldenburg and Caspian pony breeds and non-equilibrium situation in Thoroughbred and Arab breeds. The overall results shows that the genetic diversity in studied samples can be used as a resource for horse breeding programs. Manuscript profile
      • Open Access Article

        6 - Genetic diversity of some wheat landraces of Zanjan province by gliadin patterns
        Arash Mohammadi Mostafa Valizadeh Mohammad Moghaddam Yusef Arshad
        In present research, genetic diversity of gliadins among 30 wheat landraces belong to region of Zanjan-Iran, was studied by Acid-PAGE technique. Totally 54 polymorphic gliadin bands were identified. The highest frequency was related to gliadin band 46 witch observed in More
        In present research, genetic diversity of gliadins among 30 wheat landraces belong to region of Zanjan-Iran, was studied by Acid-PAGE technique. Totally 54 polymorphic gliadin bands were identified. The highest frequency was related to gliadin band 46 witch observed in 90% of wheats. Also gliadins 12.5 and 15.5 were observed in 86.7% of the wheats. Genetic diversity of the wheats based on total gliadin found to be high (H=0.967). Highest and Lowest genetic variation related to omega-gliadins (H=0.917) and gamma-gliadins (H=0.781) respectively. Wheats of Zanjan showed diversity in terms of gliadin bands number (11-25 bands). The entire wheats, showed specific gliadin pattern. Totally, 29 omega-gliadin patterns, 15 gamma-gliadin patterns, 22 beta-gliadin patterns and 23 alpha-gliadin patterns were observed. Cluster analysis could separate three tetraploid genotypes TN-11372, TN-11734, TN-11743 from hexaploids. These results indicate that gliadin proteins analysis is useful for evaluating of genetic diversity, characterizing of wheat genotypes and separating of different ploidy levels. Manuscript profile