Anti-yeast comparative activities of aqueous and alcoholic extracts of black tea (Camellia sinensis) and grape seed (Vitis vinifera L.) on Malassezia pachydermatis (ATCC 10231) with that of the Clotrimazole

Subject Areas : microbiology

Azam Ghrbannia Delavar ¹ , niosh Qhasemi ² , maryam onsinezhad ³ , masoud hashemi karoii ⁴ , issa gholampour azizi ⁵

1 - Assistant professor, Department of Biology, Payame Noor University, Tehran, Iran
2 - Gradated student, Faculty of Veterinary Medicine, Babol Branch, Islamic Azad University, Babol, Iran
3 - Gradated student, Faculty of Veterinary Medicine, Babol Branch, Islamic Azad University, Babol, Iran
4 - Faculty of Veterinary Medicine, Babol Branch, Islamic Azad University, Babol, Iran.
5 - Faculty of Veterinary Medicine, Babol Branch, Islamic Azad University, Babol, Iran

Received: 2022-07-29 Accepted : 2022-09-05 Published : 2022-09-20

Keywords: Black Tea, Antimicrobial, Grape Seed, Malassezia pachydermatis, clotrimazole,

Abstract :

In recent years, resistance to a variety of drugs has developed due to the indiscriminate use of antimicrobials. The aim of the present study was to compare aqueous, ethanolic and methanolic extracts of black tea(Camellia sinens) and grape seed (Vitis vinifera L.) on Malassezia pachydermatis (ATCC 10231) by disk, well, Minimum Inhibitory Concentration (MIC) and Minimum Fungicidal Concentration (MFC) methods. For black tea MIC was 25(103) µg/ml for the aqueous extract, 6250 µg/ml for the ethanolic extract and 125(102) µg/ml for the methanolic extract. MFC was 0 for the aqueous extract, 125(102) µg/ml for the ethanolic extract and 25(103) µg/ml for the methanolic extract. For grape seed MIC was 1250 µg/ml for the aqueous extract, 1250 µg/ml for the ethanolic extract and 33333 µg/ml for the methanolic extract. MFC was 16667 for the aqueous extract, 125(102) µg/ml for the ethanolic extract and 23611 µg/ml for the methanolic extract. The Clotrimazole diameter the zone of inhibition on Malassezia pachydermatis was determined between 35 and 47 mm in the disk and 40 to 49 mm in the well. In all the extracts, an increasing trend of the zone of inhibition was observed with the increase in the volume of consumed extracts. Therefore, the extracts of these two plants have an accessible and natural antibiotic effect, but compared to Clotrimazole, a smaller the zone of inhibition appeared. However, the anti- yeast activity of grape seed extracts is more than that of black tea.