Isolation and identification of gram-negative bacteria from patients with urinary tract infections referred to Savadkooh laboratory and determination of their drug resistance pattern from the beginning of October 1400 to the end of December 1400
Subject Areas : microbiologyseyedmohammadmahdi mousavi 1 , mojtaba fallah 2 , neda Jafari Jushqhan 3
1 - M.Sc. Student of Pathogenic Microbes, Department of Microbiology, Central Faculty, sana Institute of Higher Education, sari, Mazandaran,Iran
2 - Bachelor of Laboratory Science, Department of Laboratory Science, School of Medicine, Islamic Azad University of Babol, Babol, Iran
3 - M.Sc. Student of Pathogenic Microbes, Department of Microbiology, Central Faculty, sana Institute of Higher Education, sari, Mazandaran
Keywords: Bacteria, Urinary tract infections, pattern of antibiotic resistance,
Abstract :
Today, microorganisms have become resistant to drugs by causing genetic mutations. The aim of this study was to determine the antibiotic resistance of gram-negative bacteria isolated from urine culture in the laboratory of Savadkuh city. The cross-sectional descriptive study was performed on gram-negative bacteria isolated from urinary tract infections in Savadkuh laboratory. Bacterial isolates were identified by microscopic examination, morphology and biochemical tests. Fifty samples of gram-negative bacteria causing urinary tract infections were isolated from 5 cases (10%) of men and 45 cases (90%) of women. According to the study, the two most common gram-negative bacteria in urinary tract infections are based on the percentage of infectivity in this study, which include: E.Coli (62%), and Klebsiella (30%) and other gram-negative (8%), They were isolated from urine samples. According to the antibiogram test, the most cases of resistance were related to the antibiotics ampicillin, nalidixic acid and cefazolin, respectively. On the other hand, the most sensitive drugs are nitrofurantoin, sulfamethoxazole trimethoprim and ciprofloxacin, respectively.
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