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      • Open Access Article

        1 - Identification of dehydration-responsive element binding transcription factor gene in Bromus japonicus under salinity stress
        mahboobeh basiri seyed mohsen mosavi nik seyed kazem sabagh davood naderi Asiyeh Siahmarguee Khodadad Mostafavi
        Invasive plant species that are characterized as weed, plants are resistant to environmental hard conditions that this feature can be used to the genetic alternation and transformation to resistant crops. The aim of this study was identification of gene involved in tole More
        Invasive plant species that are characterized as weed, plants are resistant to environmental hard conditions that this feature can be used to the genetic alternation and transformation to resistant crops. The aim of this study was identification of gene involved in tolerance to abiotic environmental stresses in the Bromus japonicus called DREB2. in this subject, salinity stress was applied via sodium chloride, Sequencing and Identification of stress resistance gene in this plant and then sequence obtained was examined. Sequence Analysis and Comparing sequence obtained with sequences this gene in other plants was performed with softwares such as BLAST, ExPasy, ELM, Clustal W, Meg Align. The result was showed that the amplified fragment belonged to part of salinity-responsive element binding-factor gene in this plant. The comparison DREB2 gene sequence with other sequences this gen in the other plant species was confirm the Bromus japonicus and Aegilops tauschii have the most similarity with 89.9% the highest likeness (the lowest genetic distance). Bioinformatics analysis of the amino acid sequence of the translated nucleotide sequence of the gene DREB2 in the Bromus japonicus through software CDD search showed that AP2 Domain area is in this sequence, These sequence was submitted to GenBank, Its accession code is KP406596. Manuscript profile
      • Open Access Article

        2 - Investigating the resistance of wild mustard (Sinapis arvenisis L.) biotypes to tribenuron methyl herbicide in wheat fields of Ramiyan Township
        Mahtab Heravi Javid Gherekhloo Asieh Siahmarguee Hossein Kazemi Saeid Hassanpour Bourkheili
        More than twenty years after registration of tribenuron methyl herbicide in Iran, there are reports on its lack of efficacy in controlling broad leaved weeds in wheat fields of Ramiyan Township. To investigate the occurrence of resistance to tribenuron methyl in wild mu More
        More than twenty years after registration of tribenuron methyl herbicide in Iran, there are reports on its lack of efficacy in controlling broad leaved weeds in wheat fields of Ramiyan Township. To investigate the occurrence of resistance to tribenuron methyl in wild mustard (Sinapis arvensis L.), a series of experiments were conducted at Gorgan University of Agricultural Sciences and Natural Resources in 2016. Putatively resistant wild mustard biotypes were gathered from wheat fields of the Township. A susceptible biotype was also gathered from the fields with no history of spraying. Putative biotypes were first screened with the recommended dose. Then, response of susceptible and resistant biotypes to various doses of tribenuron methyl herbicide was investigated. The results of dose-response assay implied occurrence of resistant to tribenuron methyl in some wild mustard biotypes. Resistance factors of RAM-R-14, RAM-R-27, RAM-R-25, RAM-R-30 and RAM-R-5 were obtained respectively 3.90, 4.10, 3.96, 2.66 and 2.36. Distribution map of fields infested with susceptible and resistant wild mustard biotypes was drawn using geographic information system and it was revealed that the gathered biotypes were observed in the fields which possessed unsuitable cropping pattern and weed management was based on chemical control. The results of the present study may be utilized to implement herbicide-resistant weed management practices as well as prevention of development of these plants to other regions. Manuscript profile
      • Open Access Article

        3 - Estimation of Cardinal Temperatures and Thermal Time Requirement for Bromus japonicus Seed Germination
        Mahboobeh Basiri Seyed Mohsen Mousavi Nik Asieh Siahmargooei Seyed Kazem Sabagh