The first report of Dourine's disease in horses with suspected clinical forms of the disease in the rural villages of Dorood city, Lorestan province, Iran

Subject Areas : Journal of Large Animal Clinical Science Research(JLACSR)

saeid Hashemi ¹ , Meisam Salarvand ²

1 - 1- Department of Parasitology, Borujard Branch, Islamic Azad University, Borujard, Iran
2 - 2- Master of parasitology, Borujard Branch, Islamic Azad University, Borujard, Iran

Received: 2022-08-19 Accepted : 2022-11-28 Published : 2022-11-22

Keywords: , , : Dourine, , horse, , Buffycoat, , Dorood,

Abstract :

Dourine is a parasitic venereal disease of horses caused by the flagellated protozoan Trypanosome equi perdum from the Trypanosomatida's order. This deadly disease, which existed in the past and is emerging again in Lorestan today, has not been investigated in Lorestan despite suspected cases.Therefore, for this purpose, venous blood samples (4 stallion samples and 9 mare samples) were collected from 13 suspected horses by referring to four villages on the outskirts of Dorood city, were collected from May 2020 to May 2021. The results of the field examination of the animal include fever of 38.5 ± 1 degrees Celsius, edema of the genital tract and rapid fatigue, and paleness of the eye mucosa, eye hyperemia (6 cases of fever and 2 cases of eye infection), cardiac tachycardia, and in one case of a stallion, a skin plaque with a diameter of 5 Centimeters were seen on the leg. From each horse, 20 ml of blood was taken from the Vedic vein, and the samples were analyzed by the method of preparing a slide from a buffy coat and measuring the hematocrit in a capillary tube. Out of 13 horses studied, 7 cases showed a hematocrit of 32 ± 1%. The average hematocrit was 28% ± 1 in 4 stallions and 30% ± 1 in 3 mares. The trypomastigote forms of the parasite Trypanosome equipurdum, with an average absolute frequency of the disease of 23.07%, were seen in three samples