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Manuscript ID : SJOAPB-2302-1401 (R2) Visit : 256 Page: 63 - 84

Article Type: Original Research

A new strain of Rhodotrula mucilaginosa producing sophorolipid biosurfactant as an effective agent in microbial oil extraction

Subject Areas : biology

Zahra Ganji 1 , Keyvan Beheshti-Maal 2 , Ahmadreza Massah 3 , Zarrindokht Emami-Karvani 4

1 - Ph.D., Department of Microbiology, Faculty of Biological Sciences, Falavarjan Branch, Islamic Azad University, Isfahan, Iran.
2 - Associate Professor, Department of Microbiology, Faculty of Biological Sciences, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
3 - Professor, Department of Chemistry, Faculty of Science, Shahreza Branch, Islamic Azad University, Shahreza, Isfahan, Iran
4 - Assistant Professor, Department of Microbiology, Faculty of Biological Sciences, Falavarjan Branch, Islamic Azad University, Isfahan, Iran

Received: 2023-02-25 Accepted : 2023-05-30 Published : 2023-04-21

Keywords: Emulsion index, Redotrula mucilaginosa, Oil dispersion, Surface Tension, sophorolipid, Microbial oil extraction, Biosurfactant,

Abstract :

Objective: Biosurfactants have wide applications in the microbiology of food and oil. The aim of this research was to investigate the production of stable biosurfactant in high temperature and salinity from yeasts isolated from oil-contaminated soil.Materials and methods: Bushnell Haas culture medium was used to screen biosurfactant producing yeasts. The presence of biosurfactant was evaluated using oil dispersion and surface tension reduction tests.Findings: The best biosurfactant producing strain of Radotrulla mucilaginosawas named GBMEIAUF1 and its 5.8s-rDNA gene sequence was registered inthe NCBI gene bank under accession number CBS11162. The results of thin layer chromatography and Fourier transform infrared spectroscopy confirmed that the extracted biosurfactant was sophorolipid with significant surface activity. Purified sophorolipid decreased the surface tension of water from 72 mN/m to 1.29 mN/m. The highest emulsification index, E24%, the extracted biosurfactant was 53% and retained 63.71 to 58.09% of its initial activity at 80 to 120 degrees Celsius. This biosurfactant also retained 82.67 and 89.41% of its initial activity at pH 10.5, and 12, 59.66% of its initial activity at 10% salinity.Conclusion: This research is the first report of sophorolipid production by the yeast Redotrula mucilaginosa.Due to its thermal stability and high pH and salinity tolerance, sophorolipid produced by Redotrula mucilaginosa can be strongly recommended as an effective emulsifying agent for application in microbial enhanced oil extraction programs as well as food industry. 

References:


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  29. Song J, Rezaee S, Guo W, Hernandez B, Puerto M, Vargas FM, Hirasaki GJ & Biswal SL. Evaluating physicochemical properties of crude oil as indicators of low-salinity–induced wettability alteration in carbonate minerals. Sci Rep. 2020; 10:1-6.
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_||_

  1. Das MD. Application of biosurfactant produced by an adaptive strain of tropicalis MTCC230 in microbial enhanced oil recovery (MEOR) and removal of motor oil from contaminated sand and water. J Pet Sci Eng. 2018; 170: 40-8.
    2.
  2. Thavasi R, Jayalakshmi S & Banat IM. Application of biosurfactant produced from peanut oil cake by Lactobacillus delbrueckii in biodegradation of crude oil. Biores Technol. 2011; 102: 3366-72.
    3.
  3. Akbari E, Beheshti‐Maal K, Rasekh B, Emami‐Karvani Z & Omidi M. Isolation and Identification of Current Biosurfactant‐Producing Microbacterium maritypicum ABR5 as a Candidate for Oily Sludge Recovery. J Surfact Deterg. 2020; 23: 137-44.
    4.
  4. Geetha SJ, Banat IM & Joshi SJ. Biosurfactants: Production and potential applications in microbial enhanced oil recovery (MEOR). Biocatal Agric Biotechnol. 2018; 14: 23-32.
    5.
  5. Mukherjee S, Das P & Sen R. Towards commercial production of microbial surfactants. Trends Biotechnol. 2006; 24: 509-15.
    6.
  6. Astuti DI, Purwasena IA, Putri RE, Amaniyah M & Sugai Y. Screening and characterization of biosurfactant produced by Pseudoxanthomonas G3 and its applicability for enhanced oil recovery. J Petrol Explor Prod Technol. 2019; 9: 2279-89.
    7.
  7. Alvarez VM, Jurelevicius D, Marques JM, de Souza PM, de Araújo LV, Barros TG, de Souza RO, Freire DM & Seldin L. Bacillus amyloliquefaciens TSBSO 3.8, a biosurfactant-producing strain with biotechnological potential for microbial enhanced oil recovery. Colloids Surf B. 2015; 136: 14-21.
    8.
  8. Amani H, Sarrafzadeh MH, Haghighi M & Mehrnia MR. Comparative study of biosurfactant producing bacteria in MEOR applications. J Pet Sci Eng. 2010; 75: 209-14.
    9.
  9. He C, Dong W, Li J, Li Y, Huang C & Ma Y. Characterization of rhamnolipid biosurfactants produced by recombinant Pseudomonas aeruginosa strain DAB with removal of crude oil. Biotechnol Lett. 2017; 39: 1381-8.
    10.
  10. Sabati H & Motamedi H. Ecofriendly demulsification of water in oil emulsions by an efficient biodemulsifier producing bacterium isolated from oil contaminated environment. Biotechnol Lett. 2018; 40: 1037-48.
    11.
  11. Lima TM, Procópio LC, Brandão FD, Carvalho AM, Tótola MR & Borges AC. Simultaneous phenanthrene and cadmium removal from contaminated soil by a ligand/biosurfactant solution. Biodegradation. 2011; 22: 1007-15.
    12.
  12. Camargo FP, Araujo ACV, Moraes EM de & et al. A comparison between cactophilic yeast communities isolated from Cereus hildmannianus and Praecereus euchlorus necrotic Fungal Biol. 2016;120: 1175–83.
    13.
  13. Konishi M, Maruoka N, Furuta Y, Morita T, Fukuoka T, Imura T & Kitamoto D. Biosurfactant-producing yeasts widely inhabit various vegetables and fruits. Biosci Biotechnol Biochem. 2014; 78: 516-23.
    14.
  14. Mnif I & Ghribi D. Review lipopeptides biosurfactants: mean classes and new insights for industrial, biomedical, and environmental applications. Pep Sci. 2015; 104: 129-47.
    15.
  15. Mousavi F, Beheshti-Maal K & Massah AR. Production of sophorolipid from an identified current yeast, Lachancea thermotolerans BBMCZ7FA20, isolated from honey bee. Curr Microbiol. 2015; 71: 303-10.
    16.
  16. Mohawesh O, Janssen M, Maaitah O & et al. Assessment the effect of homogenized soil on soil hydraulic properties and soil water transport. Eurasian Soil Sci. 2017; 50: 1077–85.
    17.
  17. Mousavi F, Beheshti-Maal K & Massah AR. Isolation of yeasts from bee bread of honey bees, Apis mellifera and evaluation of its ability to produce sophorolipid biosurfactant. Iranian J Med Microbiol. 2014; 8: 44–53.
    18.
  18. Salehizadeh H & Mohammadizad S. Microbial enhanced oil recovery using biosurfactant produced by Alcaligenes faecalis. Iranian J Biotechnol. 2009; 7: 216-23.
    19.
  19. Sen S, Borah SN, Bora A & Deka S. Production, characterization, and antifungal activity of a biosurfactant produced by Rhodotorula babjevae Microb Cell Fact. 2017; 16: 95.
    20.
  20. Harju S, Fedosyuk H & Peterson KR. Rapid isolation of yeast genomic DNA: Bust n'Grab. BMC Biotechnol. 2004; 4: 8.
    21.
  21. Akbari E, Beheshti-Maal K & Nayeri H. A novel halo-alkalo-tolerant bacterium, Marinobacter alkaliphilus ABN-IAUF-1, isolated from Persian Gulf suitable for alkaline lipase production. Int J Environ Sci Technol. 2018; 15: 1767-76.
    22.
  22. Chen J, Song X, Zhang H & Qu Y. Production, structure elucidation and anticancer properties of sophorolipid from Wickerhamiella domercqiae. Enz Microb Technol. 2006; 39: 501-6.
    23.
  23. Kurtzman CP, Price NP, Ray KJ & Kuo TM. Production of sophorolipid biosurfactants by multiple species of the Starmerella (Candida) bombicola yeast clade. FEMS Microbiol Lett. 2010; 311: 140-6.
    24.
  24. Thaniyavarn J, Chianguthai T, Sangvanich P, Roongsawang N, Washio K, Morikawa M & Thaniyavarn S. Production of sophorolipid biosurfactant by Pichia anomala. Biosci Biotechnol Biochem. 2008; 72: 2061-8.
    25.
  25. Fontes GC, Fonseca Amaral PF, Nele M & Zarur Coelho MA. Factorial design to optimize biosurfactant production by Yarrowia lipolytica. J Biomed Biotechnol. 2010; 23: 1-8.
    26.
  26. Luna JM, Rufino RD, Sarubbo LA, Rodrigues LR, Teixeira JA & de Campos-Takaki GM. Evaluation antimicrobial and antiadhesive properties of the biosurfactant Lunasan produced by Candida sphaerica UCP 0995. Curr Microbiol. 2011; 62: 1527-34.
    27.
  27. Almeida DG, Soares da Silva RD, Luna JM, Rufino RD, Santos VA & Sarubbo LA. Response surface methodology for optimizing the production of biosurfactant by Candida tropicalis on industrial waste substrates. Front Microbiol. 2017; 8: 157.
    28.
  28. Camargo FP, Menezes AJ, Tonello PS, Dos Santos AC & Duarte IC. Characterization of biosurfactant from yeast using residual soybean oil under acidic conditions and their use in metal removal processes. FEMS Microbiol Lett. 2018; 365(10): fny098.
    29.
  29. Song J, Rezaee S, Guo W, Hernandez B, Puerto M, Vargas FM, Hirasaki GJ & Biswal SL. Evaluating physicochemical properties of crude oil as indicators of low-salinity–induced wettability alteration in carbonate minerals. Sci Rep. 2020; 10:1-6.
    30.
  30. Mahmoud M, Elkatatny S & Abdelgawad KZ. Using high- and low-salinity seawater injection to maintain the oil reservoir pressure without damage. J Petrol Explor Prod Technol. 2017; 7: 589-96.
    31.

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