Effects of various isolating methods of blastomeres from two cell mouse embryos on their developmental potential to blastocyst stage in Vitro

Subject Areas : biology

Received: 2013-02-26 Accepted : 2012-05-29 Published : 2012-09-22

Keywords: Blastocyst, two cell mouse embryo, Isolating methods, Isolated blastomere,

Abstract :

Aim: The purpose of this study was to investigation and compares the developmental potential ofisolated blastomeres from two cell embryo by four different isolating methods.Material& methods: In order to remove Zona Pellucida and isolating blastomeres, physicochemicalmethods including exposure to tyrode acid, Hyaluronidase, EDTA and mechanical force were used. Theblastomeres were cultured. Number of degenerated and abnormal embryos counted and diameter andtotal cell number of blastocysts were measured.Results: Degenerated rates of blastomeres were 42.85, 57.5, 62.48 and 61.60 in mechanical, tyrode acid,EDTA and Hyaluronidase groups, respectively. It was lower in mechanical group than the others groups(P< 0.05).Conclusion: In mechanical group, there were lesser negative influences on developmental potential ofisolated blastomeres from two cell mouse embryos to blastocyst stage. Conclusion: In mechanical group, there were lesser negative influences on developmental potential ofisolated blastomeres from two cell mouse embryos to blastocyst stage.