Investigating the effect of Portulaca oleracea L. nanoparticles and Lactobacillus plantarum probiotic bacteria extract on CacoII cell line on the expression of JAKI and JAKII genes.
Subject Areas : biology
1 - Department of Biology, QoMS.C., Islamic Azad University, Qom, Iran.
Keywords: : Probiotics, Lactobacillus plantarum, colon cancer, Portulaca oleracea, CacoII, JAKII, JAKI,
Abstract :
Introduction: Portulaca oleracea nanoparticles and probiotic bacteria, which are useful microorganisms, which, by affecting the digestive enzymes of animals and humans, inhibit carcinogens in the body and laboratory conditions and compounds that induce cancer and tumors in laboratory animals, play an effective role in They perform to fight cancer. The purpose of this study is to investigate the effect of Portulaca oleracea purslane nanoparticles and Lactobacillus plantarum probiotic bacteria extract on CacoII cell line on the expression of JAKI and JAKII genes.
Materials and methods: In this study, which was conducted experimentally, the supernatant was isolated from the culture of Lactobacillus plantarum. Then, the effects of the cytotoxicity of the supernatant isolated from the bacteria and Portulaca oleracea purslane nanoparticles as well as the bacteria on the CacoII colon cancer cell line during The time of 24, 48 and 72 hours was checked using MTT method. Also, the expression of JAKII and JAKII genes was checked using Real Time PCR method.
Results: Supernatant containing Lactobacillus plantarum bacteria reduces cell proliferation. The obtained results showed that the inhibitory percentage of Lactobacillus plantarum supernatant on CacoII cancer cells, JAKI genes had a significant increase in expression during 48 and 72 hours. JAKII genes had a significant decreased in expression during 48 and 72 hours
Conclusion: Lactobacillus plantarum bacteria and Portulaca oleracea purslane nanoparticles can be used to create a new therapeutic solution with high effect, low side effects, biologically safe and less expensive for the treatment and prevention of colon cancer
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