Detection of VanA, VanB, and VanC Genes in Enterococcus Species Isolated from Fecal sample of Patients in Qom province, Iran (Short Report)

Subject Areas : microbiology

Ashkan Dirbazian ¹ , Samaneh Rouhi ² , Pegah Shakob ³

1 - MSc of Microbiology, Department of Microbiology, Islamic Azad University Qom Branch, Qom, Iran.
2 - Clinical Research Development Unite of Rouhani Hospital, Babol University of Medical Sciences, Babol, Iran
3 - Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.

Received: 2021-04-10 Accepted : 2021-09-10 Published : 2021-09-23

Keywords: fecal samples, Enterococcus, Vancomycin, resistance genes,

Abstract :

Background: Intrinsic resistance of Enterococci to vancomycin and transmission to other gram-positive bacteria is a matter of concern. Therefore, the aim of this study was to identify vancomycin resistance genes in patients-isolated Enterococci.Material and Methods: A total of 100 fecal samples were collected from patients referred to health centers in Qom province in 2018. Microbiological tests were used to identify Enterococci. The pattern of antibiotic resistance was investigated by disk diffusion method. PCR was used to detect vanA, vanB, and vanC genes in Enterococci.Results: 60 Enterococci (60%) were isolated in this study. The highest antibiotic resistance was related to the ciprofloxacin (66.6%). The highest and lowest frequencies were for the vanC (15%) and vanA (5%) genes, respectively.Conclusion: In the present study, resistance to vancomycin and other antibiotics, and the presence of van genes in resistant strains were observed. It is necessary to control and prevent the spread of enterococci in hospital and non-hospital settings.