The investigation of single nucleotide polymorphism rs2286663 in human Insulin-like 3 gene(INSL3),related to male infertility by RFLP PCR method

Subject Areas : genetic

Asieh fotoohi ¹ , رودابه بهزادی اندوهجردی ² , مسعود زندی ³

1 - Faculty of Basic Sciences. tehran cnnter university
2 - عضو هیئت علمی، واحد تهران مرکس، دانشگبه آزاد اسلامی، تهران، ایران
3 - عضو هیئت علمی، واحد تهران مرکس، دانشگبه آزاد اسلامی، تهران، ایران

Received: 2017-07-23 Accepted : 2017-11-06 Published : 2018-02-20

Keywords: INSL3 gen, azoospermia, Key Words: infertility,

Abstract :

ABSTRACT: Infertility is the inability of a couple to conceive after one year of consecutive sexual intercourses without birth control methods. Infertility is a multifactorial syndrome with several genetical or nongenetical reasons. The possible causes for infertility are still unknown for more than 25 percent of cases (idiopathic infertility). Recently a product of Leydig cells termed Leydig insulin –like hormone (INSL3)has been proposed as a putativa trophic hormone of the first part of descent.Absence of Insl3 in male mice results in bilateral cryptorchidism and mutations involving this gene may be a cause of azoospermia in man.this gene has several SNP .For this reason at this research rs2286663 is investigated. For this purpose, the number of 80 infertile men and 80 healthy men (as a control) was tested. Using a standardRFLP PCR method, the presence of the mutant was analyzed. The statistical results were not significant. Thus, no significant relationship between the mutation and idiopathic infertility in males has been detected. However, the results may be different by selecting the different genetic pool or significant changes in a population size.