Molecular detection of DHA beta-lactamase gene in Pseudomonas aeruginosa isolated from skin samples in Qom

Subject Areas : microbiology

Horisadat Hejazi ¹ , Mohsen Zargar ² , mehrdad mamarian ³

1 - Department of Microbiology,Qombranch,Islamicazad univercity,Qom,Iran
2 - 1-Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran
3 - 1-Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran

Received: 2019-05-08 Accepted : 2019-12-05 Published : 2019-10-23

Keywords: Pseudomonas aeruginosa, skin samples, DHA, Qom City,

Abstract :

Introduction Pseudomonas aeruginosa can infect virtually all tissues in the body. this bacteria can be colonized in burns, wounds, corneal tissue, urinary tract and lungs, and after entering blood, cause septicemia or localized lesions in other parts of the body. Today, due to the untapped use of effective drugs On the pseudomonas aeruginosa, this bacterium is strongly resistant to antibiotics. The purpose of this study was to find the gene for resistance to beta-lactams in pseudomonas aeruginosa isolated from skin samples. materials and methods: A total of 100 isolates of Pseudomonas were used in Microbiology Bank of Microbiology Research Laboratory of Islamic Azad University of Qom. After cultivation and biochemical confirmation of isolates, genomic extraction was performed first to detect these genes and then detected by PCR with DHA genes. Results: 45 isolates resistant to imipenem; 22 cases were positive in the double-synergistic disks. Six isolates were positive for DHA gene. Conclusion: Increasing resistance to Pseudomonas aeruginosa causes problems in treating patients and a periodic review of drug allergy for inappropriate treatment.