Determine the antimicrobial susceptibility of Pseudomonas aeruginosa strains isolated from raw milk

Subject Areas : microbiology

faeze zadsafar ¹ , MOHSEN ZARGAR ² , سید سهیل آقایی ³

1 - Student
2 - هیئت علمی دانشگاه آزاد قم
3 - هیئت علمی دانشگاه آزاد قم

Received: 2016-04-26 Accepted : 2016-06-28 Published : 2013-06-22

Keywords: Pseudomonas aeruginosa, microbial drug resistance, Multiple drug resistance, dairy products,

Abstract :

AbstractBackground and Objectives: Pseudomonas aeruginosa, a pathogen bacteria, that is resistance to antibiotics and can be used in patients with immune deficiency and cause disease. This study aimed to determine the antimicrobial susceptibility of Pseudomonas aeruginosa strains isolated from raw milk and multiple resistance of the isolates and ESBL isolates.Method: Initially, 117 Pseudomonas aeruginosa isolates from dairy products were confirmed by the standard methods of microbiology and then evaluated the antibiotic resistance of 10 strains against 10 antibiotic which selected from different categories according to the CLSI standard. Results: In the study of antibiotic resistance Ceftazidime antibiotic showed most Antibiotic resistance and amikacin antibiotic polymyxin B antibiotic resistance and lowest, respectively.27 isolates (23%), multiple drug resistance (MDR), the three classes of antibiotics showedand 86 isolates were resistant to the ceftazidime antibiotic (73.5%) isolates, of which 28 were positive ESBL (32.55%). Conclusion: Due to high prevalence Pseudomonas aeruginosa in raw milk, and existence of antibiotic resistance genes in bacteria, it is necessary to apply appropriate strategies for livestock health control, to prevent the spread of bacteria.