The Protective Effects of Royal Jelly on the Histomorphometric ,Hormonal Alternation and Oxidative Stress in Ofloxacin Treated Male Rat
Subject Areas :
1 - استادیار گروه زیست شناسی، دانشکده علوم پایه، دانشگاه فرهنگیان، تهران. ایران
Keywords: Histomorphometric, Oxidative stress, royal jelly, Rat, Keywords:Ofloxacin,
Abstract :
Inroduction & Objective: Royal jelly is a compound that is produced by the worker honey bees. This compound has antioxidant activity and protective effects against oxidative stress. The aim of this study were to determine protective role of royal jelly on decrease side effects of ofloxacin and return reproductive function near normal rang in ofloxacin treated male ratMaterial and Methods:In this experimental work used 32 male albino rats that each has body weight between 160-18o gand accidentally divided to 4 groups: ofloxacin, royal jelly , ofloxacin with royal jelly and control. Each group included 8 rats. Dosage for ofloxacin group was 216 mg/kg.BW ofloxacin and for royal jelly group was 100 mg/kg.BW royal jelly. Royal Jelly with ofloxacin group received 216 mg/kg ofloxacin and100 mg/kg royal jelly. Control group received distilled water at dose of 216 mg/kg.BW. All groups received dose - related daily for 28 days orally. Data analyzed by one-way ANOVA and used Turkey HSD Post-test to compare the significance of difference. Results: After exposure ofloxacin results revealed thatdiameter of seminiferous tubules, thickness of epithelium seminiferous tubules, weight testes,FSH, LH, testosterone, TTM ,TAOCand RI in compared to control significance decline,but distance between seminiferous tubule ,TDI , SI, MDA and NO significanceincreased. All parameters were improved to near normal rang level after exposure royal jelly.Conclusion:This investigation revealed that ofloxacin has negative effect on histomorphometry and reproductive function in mature male rats,but at the same time if you use royal jelly it is protects against ofloxacin and retune to normal rang.