Study Of(LC50 96 h) and Histopathological Lesions Of Nitrate in TheGill, Liverand Kidney Of Persian Sturgeon Acipenser persicus
Subject Areas :Arash Jamshidi 1 , Hossein Khara 2 , ذبیح الله Pazhnd 3
1 - دانشگاه آزاد اسلامی واحد لاهیجان، دانشکده منابع طبیعی، دانش آموخته کارشناسی ارشد گروه شیلات، لاهیجان. ایران.
2 - دانشگاه آزاد اسلامی واحد لاهیجان، دانشکده منابع طبیعی، استادیار گروه شیلات، لاهیجان. ایران.
3 - انستیتو بین المللی ماهیان خاویاری،بخش فیزیولوژی،سنگر، رشت. ایران.
Keywords: Persian sturgeon(Acipenser persicus), Acute toxicity, histopathology of Gill, Nitrate, Liver and Kidney,
Abstract :
Inroduction and Objective: Spreading ofpollutants, including nitratecausingacuteor semi-acute poisoningespecially in fry fishin water, its concentrationincause interferencephysiological functionsand causeof unknown death. purpose of thispilotstudy was to determinesafelevels ofnitratesin the waterandacuteeffectsonthe gill ,liver andkidneytissuesofsturgeon(Acipenser persicus) which isa valuablefish inthe Caspian Sea.Materials and Methods: The concentration of the initial inception of LC10, LC50 and LC90 during 24, 48, 72 and 96 hours according to OECD method, for the nitrate on larve of Acipenser persicus (weighing 2to3g) compared with the control group , were measured. As for the larve, Acipenser persicus 750, 773, 798, 823 and 850 mg per liter of nitrate was calculated.Results: The LC10 of nitrate 24, 48, 72 and 96 was 775.6097, 716.2509, 677.9556 and 669.7878 mg per liter, the LC50 nitrate 909.7434, 825.9397, 793.645 and 764.5538 mg per liter and the LC90 nitrate, 1067.074, 952.4265, 929.076 and 872.728 mg per liter, respectively.Resultsshowed that the gills phenomena such as congestion, swelling of the primary lamella, hyperplasia, adhesions secondary lamella, hemorrhage and necrosis were observed after exposure to nitrate within 96 hours. It should be noted that the severity of injuries getting higher in the plots treated 1 to 5 treatments.In theliverof Persiansturgeonafterexposure adverse effectssuch ashyperemia, cellularnecrosis, bilerecordandcellatrophywas observed, buttheir intensitywasvariedineach treatmentand even insometreatmentsmay be oneofthecomplicationsdo not seen. In thekidneyof Persiansturgeon complications such as hyperemiaandbleeding, melanomacrophage, increasedurinaryspaceandhypertrophywereobserved. Themost common complication were in treatments4and5.Conclusion: According topresentstudy Persiansturgeonissensitivespecies to nitrateandnitriteconcentrationsshould beconsideredingrowthstages. That not bemore thandeclared.