Effects of glutamine supplementation on the relative expression of Pax3 and Pax7 genes in slow-twitch muscle tissue of male rats after a session of intense resistance exercise
Subject Areas : Molecular detection of biochemical and genetic markersShahla Moradi 1 , طاهره باقرپور 2 * , Nematollah Nemati 3
1 - Member of the scientific faculty of Islamic Azad University, Damghan branch
2 - استادیار گروه علوم ورزشی دانشگاه آزاد واحد دامغان
3 - Department of sport and exercise, Islamic Azad University Damghan Branch
Keywords: Glutamine supplementation, resistance training, satellite cells, myogenic genes, muscle damage.,
Abstract :
Background & Aim: Glutamine is one of the most abundant free amino acids in skeletal muscle and plays a key role in muscle regeneration and repair. It has been shown to reduce muscle damage from intense resistance training and support recovery. Intense exercise activates satellite cells and increases the expression of myogenic genes such as PAX3 and PAX7, markers of muscle stress. However, limited research has focused on slow-twitch muscle fibers. This study aimed to evaluate the effects of glutamine supplementation on the expression of these genes in the soleus muscle of male rats after a single session of intense resistance training.
Materials & Methods: Thirty 8-week-old male Wistar rats were maintained under controlled conditions and randomly divided into three equal groups after weight matching. Glutamine was administered orally once daily. The soleus muscle tissue was analyzed using PCR, and relative gene expression was assessed by comparing target and reference genes.
Results: The expression levels of PAX3 and PAX7 were higher in the intense training group compared to the group that also received glutamine. There was no significant difference in PAX3 expression between the two training groups. However, PAX7 expression was significantly lower in the glutamine-supplemented group than in the non-supplemented one.
Conclusion: A single session of intense resistance training increases the expression of myogenic genes in slow-twitch muscle fibers. Glutamine supplementation appears to moderate this response, potentially offering a protective effect against muscle damage.
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