Investigating the Antimicrobial Activity of Peperin Isolated from Black Pepper on Staphylococcus Aureus Isolated from the Throat and Nose
Subject Areas : Journal of Quality and Durability of Agricultural Products and Food StuffsSaeide Saeidi 1 , Mehdi Jahantigh 2 , Razvane Behzad Mehr 3 , Zahra Beigomi 4
1 - M.S, Agricultural Biotechnology Institute, University of Zabol, Zabol, Iran
2 - Associate Professor, Clinical Pathology, Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran
3 - Zahedan University of Medical Sciences, Zahedan, Iran
4 - Assistant Professor, Zahedan University of Medical Sciences, Zahedan, Iran
Keywords:
Abstract :
Due to the increasing resistance of pathogenic bacteria to common antibiotics, researchers are looking for antimicrobial agents of plant origin as alternative drugs. The aim of this study is to investigate the antimicrobial activity of peperin isolated from black pepper on Staphylococcus aureus isolated from the nose and throat. Sampling was done from the pharynx and nose of the patients and 9 strains of Staphylococcus aureus were isolated and purified. The minimum inhibitory concentration (MIC) and the minimum lethal concentration (MBC) in six concentrations were evaluated by dilution method in liquid medium on the determined bacteria. The results of this study showed that the lowest inhibitory concentration was equal to 8 μg/ml, which inhibited one strain, while the highest concentration was 512 μg/mg, which inhibited one strain. Staphylococcus aureus isolated the most It was resistant to trimethoprim (88.8%), ampicillin (77.7%), tetracycline (77.7%), erythromycin (66.6%) and ceftazidime (22.2%). The results of this study showed that piperine present in black pepper has a very strong inhibitory effect on Staphylococcus aureus bacteria isolated from the nose and throat.
_||_
1- Ravindran P. Black pepper: Piper nigrum CRC Press, Boca Raton, Fla. 2003; ISBN 9789057024535.
2- Hirasa K, Takemasa M. Spice science and technology CRC Press, Boca Raton, Fla. 1998; ISBN 9780824701444.
3- Kozukue N, Park MS, Choi SH, Lee SU, Ohnishi-Kameyama M, Levin CE, et al. Kinetics of light-induced cis-isomerization of four pipe-rines and their levels in ground black peppers as determined by HPLC and LC/MS.J Agric Food Chem, 55;2007:7131-7139. 10.1021 /jf070831p.
4- Zachariah TJ, Parthasarathy VA. Black pep-per. Chem spices. 196; 2008: 21. ISBN-13:9781 845934057.
5- Pruthi JS. Major spices of India: crop manage-ment and post-harvest technologyMajor Spices India Crop Manag Post-Harvest Technol; 1993.
6- Correa EA, Högestätt ED, Sterner O, Eche-verri F, Zygmunt PM.In vitro TRPV1 activity of piperine derived amides. Bioorg Med Chem. 2010;18: 3306-3299.
7- Hlavačková L, Janegová A, Uličná O, Janega P, Černá A, Babál P. Spice up the hypertension diet-curcumin and piperine prevent remodeling of aorta in experimental L-NAME induced hypertension. Nutr Metab (Lond). 2011;8:72.
8- Pradeep CR, Kuttan G. Effect of piperine on the inhibition of lung metastasis induced B16F-10 melanoma cells in mice. Clin Exp Metastasis. 2002;19:703-708.
9- Li S, Lei Y, Jia Y, Li N, Wink M, Ma Y. Piperine, a piperidine alkaloid from Piper nigrum re-sensitizes P-gp, MRP1 and BCRP dependent multidrug resistant cancer cells.Phytomedicine. 2011;19:83-87.
10- Meghwal M, Goswami TK. Piper nigrum and piperine: an update Phyther Res. 2013;27: 1121-1130.
11- Zarai Z, Boujelbene E, N. Ben S, Gargouri Y, Sayari A. Antioxidant and antimicrobial activities of various solvent extracts, piperine and piperic acid from Piper nigrum. LWT-Food Sci Technol. 2013;50: 634-641.
12-Tavares WS, Cruz I, Petacci F, Freitas SS, Serratilde JE, Zanuncio JC. Insecticide activity of piperine: Toxicity to eggs of Spodoptera frugiperda (Lepidoptera: Noctuidae) and Diat-raea saccharalis (Lepidoptera: Pyralidae) and phytotoxicity on several vegetables. J Med Plants Res. 2011;5:5301-5306.
13-Storz P. Reactive oxygen species in tumor progression. Front Biosci. 2005;10: 1881-1896.
14-Bano G, Amla V, Raina RK, Zutshi U, Cho-pra CL. The effect of piperine on pharm-acokinetics of phenytoin in healthy volunteers. Planta Med. 1987;53:568-569.
15-Khatri S, Ahmed FJ, Rai P. Formulation and evaluation of floating gastroretentive capsules of acyclovir with piperine as a bioenhancer. Pharma Innov. 2015;3: 78.
16-Khatri S, Awasthi R. Piperine containing floating microspheres: an approach for drug targeting to the upper gastrointestinal tract. Drug Deliv Transl Res. 2016;6: 299-307.
17-Schito GC. The importance of the deve-lopment of antibiotic resistance in Staphylo-coccus aureus. Clinical Microbiology and Infec-tion. 2006; 12(1): 3-8.
18-Ehling-Schulz M, Fricker M, Scherer S. Bacillus cereus the causative agent of an emetic type of food-borne illness molecular nutrition and food research. 2004; 48(7): 87-479.
19-Bode LG, Kluytmans JA, Wertheim HF, Bogaers D, Vandenbroucke-Grauls CM, et al. Preventing surgical-site infections in nasal carr-iers of Staphylococcus aureus. The New England journal of medicine. 2010; 362(1): 9-17.
20-Zhu J, Lu C, Standland M. Single Mutation on the surface of Staphylococcus aureus A can disrupt its dimerization. Biochemistry. 2008; 47(6): 74-1667.
21-Mohsenipour Z, Hassanshahian M. Com-parison of Antimicrobial Effects of Pomegranate Alcohol Extract on Single and Biofilm Form of Six Pathogenic Bacteria. Journal of Babol Univ-ersity of Medical Science. 2014; 17(1):77-84.
22-Mirza ZM, Kumar A, Kalia NP, Zargar A, Khan IA. Piperine as an inhibitor of the Mde A efflux pump of Staphylococcus aureus. Journal of Medical Microbiology. 2011; 60: 1472-1478.
23-Zarin Qalam Moghadam C, Sattari M, Zarin Qalam M. Effect of alcoholic extract of black pepper, red pepper and Shirazi thyme on inh-ibition of Staphylococcus aureus DNase enzyme. Medicinal Plants.1386; 6(12).