The frequency of genes in integron class 1, 2 and 3 in uropathogenic Escherichia coli strains isolated from diabetic patients in Shahrekord city
Subject Areas : microbiologyNegin Deli Sarraki 1 , fatemeh Khodaverdipour 2 , Nazila Arbab Soleimani 3
1 - 1- Master's degree in microbiology, Faculty of Basic Sciences, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran.
2 - Research Center of Nutrition and Organic Products (R.C.N.O.P), Islamic Azad University, Shahrekord Branch,Shahrekord ,Iran.
3 - 3- Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Damghan Branch, Damghan,Iran
Keywords: Diabetic pateients, Escherichia coli, Integron, Urinary Tract nfection,
Abstract :
The most common uropathogenic strains of Escherichia coli strains are pathogenic in humans. The most important feature of uropathogenic Escherichia coli strains, the ability of colonization on the surface of host is the euro epithelium cells.
The present study took place on 300 urine samples of patients with diabetes mellitus type 2 suspected urinary tract infections, referred to clinical laboratories of Shahrekord city, Iran in 1394. 5 to 10 cc of urine samples from diabetic patients were collected and by calibrated loop, 0.01 ml of urine cultured in Mac kanky agar media. After performing PCR test were examined to 16srRNA Escherichia coli gene, all samples tested for the presence of class 1, 2 and 3 integron genes at the present of specific primers.
Of 300 diabetic patients UTI diagnosed in 100 samples. In 51 patients (51%) E.coli was diagnosed. Results indicate that class 1 integron was seen in 30 isolations (58.82%), class 2 in 20 (39.2%) and the third class in 5 ones (9.8%). Most resistance reported to ampicillin (66.66%) and the lowest resistance reported to Nitrofurantoin (1.96%).
Based on statistical analysis had a significant relationship was observed between resistance to gentamicin, imipenem and Nitrofurantoin with class 1 integron (p-value< 0/05).
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