Investigation of the Effect of Strontium Ion Content on Thermal, Bioactivity, Antibacterial Properties and Behavior of MC3T3-E1 Osteoblast Cells in Silicate-Based Bioactive Glass

Subject Areas :

Noushin Nankali ¹ , Amirhossein Moghanian ² , Morteza Saghafi Yazdi ³

1 - Master student, Materials Engineering, Department of Materials Engineering and Metallurgy, Imam Khomeini International University, Qazvin, Iran.
2 - Assistant Professor, Department of Materials and Metallurgy Engineering, Imam Khomeini International University, Qazvin, Iran
3 - Assistant Professor, Department of Materials and Metallurgy Engineering, Imam Khomeini International University, Qazvin, Iran

Received: 2021-02-18 Accepted : 2021-06-27 Published : 2021-10-23

Keywords: Bioactive Silicate Glass Hydroxyapatite Strontium Antibacterial MC3T3, E1 Osteoblast Cells,

Abstract :

Bioactive glass (BG), is able to bind to body tissues, in this regard, it can be considered suitable material for applications such as bone tissue engineering. In this study, we first synthesized and studied the structural changes, biocompatibility, in vitro bioactivity, biological behavior and antibacterial properties of silicate-based BG containing with strontium. To evaluate the bioactivity, the BG powder was placed in a simulated body fluid (SBF) solution for 1, 3, 7 and 14 days and then before and after the mentioned time periods, the changes and the process of forming a hydroxyapatite (HA) layer on their surface was studied by using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), release rates of various ions elements, pH changes and scanning electron microscopy (SEM). The results of X-ray diffraction tests and infrared spectroscopy studies confirmed the formation of crystalline HA layer on the BG surface. Moreover, the results of live/dead assay, nucleus microstructure and actin microfilaments of MC3T3-E1 osteoblast cells showed that 5% of strontium in silicate-based bioactive glass led to the growth, proliferation and activity of MC3T3-E1 osteoblast cells. The results of cytotoxicity test and evaluation of alkaline phosphate activity showed that substitution of strontium instead of calcium in silicate-based bioactive glass composition not only did not cause cytotoxicity but also caused significant proliferation and activity of MC3T3-E1 osteoblast cells. Meanwhile, improvements in the antibacterial properties of strontium-containing bioactive glass against MRSA bacteria were observed in comparison with strontium-free bioactive glass.

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