investigating the flora of the eye and the lower area of the eye in healthy and epiphora affected dogs by method culture and PCR

Subject Areas : Smal Animal Medicine

morteza razaghi manesh ^{1 *} , erfan dayani ² , alireza salmani ³ , Mahan Yaghmaee ⁴

1 - Department of Veterinary Medicine, Faculty of Agriculture and Veterinary Medicine, Shushtar Branch, Islamic Azad University, Shushtar,
2 - Graduate of Doctor of Veterinary Medicine, Islamic Azad University, Shushtar Branch, Iran
3 - Doctor of Veterinary Medicine Student, Islamic Azad University, Shushtar Branch, Iran
4 - Doctor of Veterinary Medicine Student, Islamic Azad University, Shushtar Branch, Iran

Received: 2025-05-05 Accepted : 2025-05-09 Published : 2025-06-04

Keywords: Epiphora, Bacterial Flora, Fungal Flora, Conjunctiva, Eyelid,

Abstract :

This study was conducted with the aim of investigating the flora of the eye and the lower area of the eye in healthy and epiphora affected dogs. A plate of SDA (saborodextrose agar) was applied to each ear for fungal culture. In summary, in the present study, the bacterial and fungal flora of the eye and under the eye in cases of epiphora were investigated. In eye samples with epiphora, various fungi including Chatomium, Pichia, Aspergillus niger, Aspergillus fumigatus, Fusarium, Candida and Aspergillus brasiliensis were identified with different frequencies. In normal eye samples, Aspergillus niger and Candida were identified, but other fungi were not present. In normal skin samples, Aspergillus fumigatus was detected in two samples, while no other fungi were found. In skin samples with epiphora, similar to eye samples with ephiora, various fungi including Chatomium, Pichia, Aspergillus niger, Aspergillus fumigatus, Fusarium, Candida and Aspergillus brasiliensis were detected with different frequencies. In normal eye samples, bacterial species such as S. intermedius and S. epidermidis were detected in all samples, while other species were found with different frequencies. In eye samples with epiphora, similar to normal eye samples, S. intermedius and S. epidermidis were detected in all samples, but other species were present with different frequencies, including S. auricularis, S. cohnii, S. xylosus, Strep. canis, Strep. agalactiae, Micrococcus nishiomiae, Coryne. aquaticum, Coryne. xerosis and Coryne. aquaticum. In normal skin samples, S. intermedius and S. epidermidis were detected in most samples with different frequencies, while other species were not reported

References:


Rosser EJ. Causes of otitis externa. Veterinary Clinics: Small Animal Practice. 2004 Mar 1; 34(2):459-68.
Gotthelf LN. Diagnosis and treatment of otitis media in dogs and cats. Veterinary Clinics: Small Animal Practice. 2004 Mar 1; 34(2):469-87.
Doyle RS, Skelly C, Bellenger CR. Surgical management of 43 cases of chronic otitis externa in the dog. Irish Veterinary Journal. 2004 Dec; 57(1):1-9.
Griffin CE. Otitis techniques to improve practice. Clinical Techniques in Small Animal Practice. 2006 Aug 1; 21(3):96-105.
Murphy KM. A review of techniques for the investigation of otitis externa and otitis media. Clinical techniques in small animal practice. 2001 Nov 1; 16(4):236-41.
Paterson S. Discovering the causes of otitis externa. In Practice. 2016 May; 38:7-11.
Ngo J, Taminiau B, Fall PA, Daube G, Fontaine J. Ear canal microbiota–a comparison between healthy dogs and atopic dogs without clinical signs of otitis externa. Veterinary dermatology. 2018 Oct; 29(5):425-e140.
Beale KM, Kunkle GA, Chalker L, Cannon R. Effects of sedation on intradermal skin testing in flea-allergic dogs. Journal of the American Veterinary Medical Association. 1990 Oct 1; 197(7):861-4.
Quinn PJ, Markey BK, Leonard FC, Hartigan P, Fanning S, Fitzpatrick E. Veterinary micro biology and microbial disease. John Wiley & Sons; 2011 Oct 7.
Święcicka N, Bernacka H, Fac E, Zawiślak J. Prevalence and commonest causes for otitis externa in dogs from two Polish veterinary clinics. Bulgarian Journal of Veterinary Medicine. 2015 Mar 1; 18(1).
Saridomichelakis MN, Farmaki R, Leontides LS, Koutinas AF. Aetiology of canine otitis externa: a retrospective study of 100 cases. Veterinary dermatology. 2007 Oct; 18(5):341-7.
Jaeger K, Linek M, Power HT, Bettenay SV, Zabel S, Rosychuk RA, Mueller RS. Breed and site predispositions of dogs with atopic dermatitis: a comparison of five locations in three continents. Veterinary dermatology. 2010 Feb; 21(1):119-23.
Fernández G, Barboza G, Villalobos A, Parra O, Finol G, Ramirez RA. Isolation and identification of microorganisms present in 53 dogs suffering otitis externa. Revista Cientifica-Universidad Del Zulia Facultad De Ciencias Veterinarias Division De Investigacion. 2006 Jan 1; 16(1):23.
Guillot J, Bond R. Malassezia yeasts in veterinary dermatology: an updated overview. Frontiers in cellular and infection microbiology. 2020:79.
Campbell JJ, Coyner KS, Rankin SC, Lewis TP, Schick AE, Shumaker AK. Evaluation of fungal flora in normal and diseased canine ears. Veterinary dermatology. 2010 Dec; 21(6):619-25.
Zur G, Lifshitz B, Bdolah‐Abram T. The association between the signalment, common causes of canine otitis externa and pathogens. Journal of Small Animal Practice. 2011 May; 52(5):254-8.