spic and rfbjB Genes studies in isolated Salmonella strains from poultry in sari

Subject Areas : clinical veterinary science

میترا Salehi, ¹ , سمیه Mohammadi, ²

1 - Departement of microbiology, Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran
2 - Postgraduate student, Departement of microbiology, Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran

Received: 2016-11-04 Accepted : 2016-11-04 Published : 2014-06-22

Keywords: PCR, Salmonella, intestinal chicken,

Abstract :

salmonella is a Gram-negative bacterium that can cause infection in humans and a wide range of animals suchas human and farm animels such as cattle, poultry, pig and reptiles, which can be transmitted to humans throughinfected food. In this study, PCR was used to detect salmonella in chicken intestine. In this study, 100 samplesof chicken intestines was prepared from aviculture around the city of Sari. All samples were examined usingbiochemical and biological methods. DNA samples was extracted by MBST kit and PCR method was employedby rfbjB and SpiC primers related to rfbjB and SpiC genes. The results of biochemical testing and culture methodson 100 samples collected from chicken intestine, 30 Salmonella was detected. Reults showed that all isolatedsamples contained SpiC gene and 50% contained rfbjB gene. these results indicate that this PCR assay is a simple,Rapid, reliable and reproducible method for identifcation of salmonella that will aid surveillance , prevention andcontrol of this pathogen