Histopathological study of osteoporotic changes after experimental creation of osteoporosis by ovariectomy in the rat

Subject Areas : Veterinary Clinical Pathology

داریوش  Mohajeri ¹ (Department of Pathobiology, Faculty of Veterinary Medicine, Islamic Azad University of Tabriz, Tabriz, Iran)
مهران  Mesgari ² (Drug Applied Research Center, Tabriz University of Medical Science, Tabriz, Iran)
Ali  Rezaie ³ (Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University of Tabriz, Tabriz, Iran)
Amin  Balila ⁴ (Graduated of Veterinary Medicine, Islamic Azad University of Tabriz, Tabriz, Iran)

Received: 2007-04-24 Accepted : 2007-11-11 Published : 2007-08-23

Keywords: Rat, Histopathology, Ovariectomy, Osteoporosis,

Abstract :

In order to study the histopathology of osteoporosis during menopause, 80 female Sprague-Dawley rats with approximate age of 10 weeks were allocated to 10 groups of 8 rats each (C1, C2 and C3 as control, S1, S2 and S3 as sham, T1, T2 and T3 as treatment groups and C0 as one pure control group). The nutritional and maintenance conditions were similar in all groups. Treatment groups (T1, T2 and T3) were bilaterally ovariectomized using right and left flank approach. Sham groups (S1, S2 and S3) underwent operation in which ovaries were exposed from the same approach but left intact. Treatment (T1, T2 and T3) and their related control groups (C1, C2 and C3) were euthanized at 5, 12 and 21 weeks respectively after ovariectomy. After necropsy, both tibia and femur bones were totally removed and fixed in 10%  buffered formalin, decalcified and tissue specimens stained with H&E for histopathological study. Microscopically, osteoporosis was prominent in treatment groups T2 and T3 although it was more severe in treatment group T3. Statistically, differences between ovariectomized groups (T1, T2 and T3) regarding the severity of osteoporosis with 99% confidence were significant (P<0.01). Significant differences between treatment group T1 and control groups were not observed (P>0.05).

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