Study of the effect of prostaglandin F2α analogue, Dinoprost, on cropus luteal tissue in pseudopregnant rabbit

Subject Areas : Veterinary Clinical Pathology

S.e Safavi ¹ , M.H Khayat Nouri ²

1 - Department of Basic Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran
2 - Department of Basic Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran

Received: 2008-05-19 Accepted : 2008-08-25 Published : 2008-05-21

Keywords: Rabbit, corpus luteum, PGF2 alpha, pseudopregnancy,

Abstract :

    In this study the effect of prostaglandin F2 alpha analogue, dinoprost, on corpus luteal tissue in pseudopregnant rabbit was investigated. For this purpose, 42 mature female rabbits were randomly divided into 6 groups of 7 animals each. In 3 groups, on day 10 of pseudopregnancy, dinoprost (1mg/kg) was injected intramuscularly and 24, 48 and 72 hours following injection respectively, the animals were necropsied. In the other three groups, normal saline solution was injected as control groups. In all groups, after blood sampling the animals were necropsied and their ovaries removed and weighed. Following sampling and fixation in 10% formalin solution, the tissue samples were stained by Hematoxylene & Eosine method and the corpora lutea were studied morphologically and morphometrically. Histomorphometrical study indicated that the diameter of corpus luteum decreased significantly (p<0.05) in all treatment groups compared with the control groups. Also progression of connective tissue, pyknotic nuclei, intercellular space and large vacuole formation increased significantly (p<0.05) in treatment groups in comparison with the control groups. Peripheral and central fatty droplets increased significantly (p<0.05) 24 and 48 hours post PGF2α injection respectively. Serum progesterone concentration decreased significantly (p<0.05) in all treatment groups as compared with the control groups. The results indicated that structural and functional luteolysis progressively increased in PGF2α treated groups compared with the control groups.  

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