Study on the effect of Fargesin on renal ischemia-reperfusion injury in male rats
Subject Areas : Veterinary Clinical Pathologymohammad amin nazari 1 , داریوش Mohajeri 2 , Mahbod Bazhban 3
1 - Graduate of Veterinary Medicine, Faculty of Veterinary Medicine, Tabriz Medical Sciences, Islamic Azad University, Tabriz, Iran.
2 - Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Tabriz Medical Sciences, Islamic Azad University, Tabriz, Iran.
3 - Graduate of Veterinary Medicine, Faculty of Veterinary Medicine, Tabriz Medical Sciences, Islamic Azad University, Tabriz, Iran.
Keywords: Fargesin, Ischemia-Reperfusion, Kidney, Rat.,
Abstract :
Renal ischemia-reperfusion (IR) injury is one of the most important causes of acute renal failure. Fargesin has anti-inflammatory properties and most recent studies have focused on its anti-inflammatory effects. The aim of the present study was to evaluate the effect of Fargesin on renal ischemia-reperfusion injury in rats. For this purpose, 21 male Wistar rats were randomly divided into three groups of seven rats each including: Sham operated group, IR group and IR plus Fargesin treatment. Fargesin (50 mg/kg) was administered through gavage for 12 weeks after left renal IR induction. After anesthesia, blood was taken from the tail vein to measure serum creatinine and urea, thereafter all rats were euthanized. The ELISA technique was used to evaluate the antioxidant status of renal tissue and inflammatory parameters. Histopathological studies were carried out by routine methods. Renal IR resulted in a significant increase in creatinine and blood urea levels compared to sham group (p<0.05), whereas administration of Fargesin significantly reduced these parameters (p<0.05). The antioxidant activity of renal tissue significantly decreased in the IR group but increased in the Fargesin treated rats (p<0.05). Fargesin significantly reduced IL-1 and TNF-α compared to IR group in renal tissue (p<0.05). Histopathology confirmed the biochemical findings. The results obtained showed that Fargesin has a beneficial and protective effect on renal IR injury. These effects of Fagersin may be due to the reduction of oxidative stress and inflammation in renal tissue.