A review of the occurance of bovine neosporosis in Iran
Subject Areas : Veterinary Clinical PathologyAhmad Nematoallahi 1 , Negar Foroozanfar 2
1 - Professor, Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
2 - D.V.M Graduate, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
Keywords: Iran, Cow, Neosporosis, Review studies,
Abstract :
Neosporosis is a disease caused by a protozoan called Neospora caninum, which is characterized by abortion in cows and nerve and muscle paralysis of various organs, especially the hind limbs of dogs. This disease was identified for the first time in 1984 in Norway. The dog is the only definitive host and also the intermediate host of protozoa and contamination can also be seen in cattle, sheep, goats, horses, and deer. The importance of neosporosis disease is related to causing abortion in ruminants (especially cows) and the economic losses caused in industrial livestock farming, which has caused many studies to be conducted in Iran and rest of the world. The disease was first reported in Iran in 2004, and since that year, many studies have been conducted about it. In this review article, an attempt has been made to introduce the protozoa that causes the disease and its evolution, as well as the methods of diagnosing the disease, and provide an overview of the studies conducted on this disease in Iran, from the beginning to the present day.
References:
Adhami, G., Hoghooghi, R. and Dalimi, A. (2014). The seroepidemiological investigation into Neospora caninum in cattle in Sanandaj, Kordestan Province. Journal of Veterinary Microbiology, 10(2): 83-92. [In Persian]
Alipour, M., Rahimi, E. and Shakerian, A. (2019). Study the prevalence of Neospora caninum in milk of ruminants by molecular method. Journal of Veterinary Microbiology, 15(2): 101-110.
Ansari-Lari, M., Rowshan, A., Jesmani, H., Masoudian, M. and Badkoobeh, M. (2017). Association of Neospora caninum with reproductive performance in dairy cows: a prospective study from Iran. Veterinary Research Forum, 8(2): 109-114.
Binaei, M., Changizi, E. and Staji, H. (2021). A serological study of Neospora caninum infection in dairy cattle in Semnan province, Iran. Journal of Veterinary Research, 76(1): 1-7. [In Persian]
Chryssafidis, A.L., Soares, R.M., Rodrigues, A.A.R., Carvalho, N.A.T. and Gennari, S.M. (2011). Evidence of congenital transmission of Neospora caninumin naturally infected water buffalo (Bubalus bubalis) fetus from Brazil. Parasitology Research, 108(3): 741-743.
Dalimi, A., Sabevarinejad, G., Ghafarifar, F. and Forouzandeh-Moghadam, M. (2014). Molecular detection of Neospora caninum from naturally infected dogs in Lorestan province, West of Iran. Archive Razi Institute, 69(2): 185-190.
Doosti, A., Khamesipour, F., Nekoei, S. and Lutvikadic, I. (2015). Survey for the presence of Neospora caninum in frozen bull’s semen samples by PCR assay. Asian Pacific Journal of Tropical Disease, 5(1): 7-12.
Dubey, J.P. (2003a). Review of Neospora caninum and neosporosis in animals. Korean Journal of Parasitology, 41: 1-16.
Dubey, J.P. (2003b). Neosporiosis in cattle. Journal of Parasitology, 89(1): 42-46.
Dubey, J.P., Sreekumar, C., Knickman, E., Miska, K.B., Vianna, M.C.B. and Kwok, O.C.H. (2004). Biologic, morphologic, and molecular characterization of Neospora caninum isolates from littermate dogs. International Journal of Parasitology, 34(10): 1157-1167.
Dubey, J.P., Schares, G. and Ortega-Mora, L. (2007). Epidemiology and control of neosporosis and Neospora caninum. Clinical Microbiology Reviews, 20(2): 323-367.
Dubey, J.P., Jenkins, M.C., Rajendran, C., Miska, K., Ferreira, L.R., Martins, J., Kwok, O.C.H. and Choudhary, S. (2011a). Gray wolf (Canis lupus) is a natural definitive host for Neospora caninum. Veterinary Parasitology, 181(2-4): 382-387.
Dubey, J.P. and Schares, G. (2011b). Neosporosis in animals—the last five years. Veterinary Parasitology, 180(1-2): 90-108.
Gharedaghi, Y. (2012). Seroprevalence of Neospora caninum in stray dogs of Tabriz Iran. Journal of Animal and Veterinary Advanced, 11(6): 723-726.
Gharekhani, J., Heidari, H. and Akbarein, H. (2012). Seroepidemiology of Neospora caninum in Iranian native and crossbreed cattle: across sectional study. Journal of Veterinary Research, 67(4): 325-329.
Gharekhani, J. and Heidari, H. (2014). Detection of anti-Neospora caninum antibodies in Iranian native cattle. Journal of Advanced Veterinary and Animal Research, 1(4): 228-231.
Gharekhani, J., Yakhchali, M. and Khaltabadi-Farahani, R. (2020). Prevalence and risk factors associated to Neospora caninum (Apicomplexa: Toxoplasmatidae) in pet dogs from Hamadan, West of Iran. Avicenna Journal of Clinical Microbiology Infection, 7(1): 22-26.
Habibi, G.R., Hashemi-Fesharki, R., Sadrebazzaz, A., Bozorgi, S. and Bordbar, N. (2005). Semi-Nested PCR for diagnosis of Neospora caninum infection in cattle. Archive Razi Institue, 59(2): 55-64.
Hadadi, M.R., Sherafati, R., Delavari, M., Arbabi, M., Gilasi, H.R. and Abed, A. (2018). Evaluation of anti-Neospora caninum antibody presence in cow’s milk in Kashan. Feyz: (Journal of Kashan University of Medical Sciences), 22(3): 333-338. [In Persian]
Haddadzadeh, H., Sadrebazzaz, A., Malmasi, A., Ardakani, H.T., Nia, P.K. and Sadreshirazi, N. (2007). Seroprevalence of Neospora caninum infection in dogs from rural and urban environments in Tehran, Iran. Parasitology Research, 101(6): 1563-1565.
Hamidinejat, H., Mosalanejad, B., Avizeh, R., Jalali, R., Hosseini, M., Ghorbanpour, M. and Namavari, M. (2011) .Neospora caninum and Toxoplasma gondii antibody prevalence in Ahvaz feral cats. Iranian.Jundishapur Journal of Microbiology, 4(4): 217-222.
Hashemi Fesharaki, S.R. (2009).Neospora caninum and neosporosis. Pasouk Publication, 1-23.
Heidari, H., Mohammadzadeh, A. and Gharekhani, J. (2014). Seroprevalence of Neospora caninum in slaughtered native cattle in Kurdistan province, Iran. Veterinary Research Forum, 5(1): 69-72.
Hoseini, A., Merat, E., Samani, S., Nezhad, S.S. and Danandeh, R. (2018). Comparison of Neospora caninum infected tissues in aborted fetal bovine by PCR. Journal of Veterinary Research, 73(3): 377-382.
Hosseininejad, M., Hosseini, F., Mahzounieh, M., Nafchi, A.R. and Mosharraf, M. (2010). Seroprevalence of Neospora caninum infection in dogs in Chaharmahal-va-Bakhtiari province, Iran. Comparative Clinical Pathology, 19(3): 269-270.
Hosseininejad, M., Hosseini, F. and Auobei, S. (2018). Seroprevalence of Neospora caninum and Toxoplasma gondii in dogs in Ahwaz, Iran. Veterinary Research and Biological Products, 122(1): 97-100. [In Persian]
Jaafari Joozani, R.A., Asadpour, R., Nematolahi, A. and Hosseininejad, M. (2012). Detection of non-spermatozoal cells of Neospora caninum in fresh semen of naturally infected bulls. Acta Scientiae Veterinariae, 40(2): 1-7.
Kamali, A., Seifi, H.A., Movassaghi, A.R., Razmi, G.R. and Naseri, Z. (2014). Histopathological and molecular study of Neospora caninum infection in bovine aborted fetuses. Asian Pacific Journal of Tropical Biomedicine, 4(12): 990-994.
Khani, M., Arabkhazaeli, F., Hosseini, S.D. and Shayan, P. (2018). Molecular detection of Neospora caninum in aborted fetuses of cattle farms in Arak. Journal of Veterinary Research, 73(4): 457-463. [In Persian]
Khanmohammadi, M. and Fallah, E. (2011). Prevalence of Neospora caninum antibodies in shepherd dogs in Sarab district, EastAzerbaijan province, Iran. African Journal of Microbiology Research, 5(28): 5062-5066.
Khodakaram-Tafti, A., Mansourian, M., Namavari, M. and Hosseini, A. (2012). Immunohistochemical and polymerase chain reaction studies in Neospora caninum experimentally infected broiler chicken embryonated eggs. Veterinary Parasitology, 188(1-2): 10-13.
Malmasi, A., Hosseininejad, M., Haddadzadeh, H., Badii, A. and Bahonar, A. (2007). Serologic study of anti-Neospora caninum antibodies in household dogs and dogs living in dairy and beef cattle farms in Tehran, Iran. Parasitology Research, 100(5): 1143-1145.
Moraveji, M., Hosseini, M., Amrabadi, O., Rahimian, A, Namazi, F. and Namavari, M. (2011). Seroprevalence of Neospora spp. in horses in South of Iran. Tropical Biomedicine, 28(3): 514-517.
Moraveji, M., Hosseini, A., Moghaddar, N., Namavari, M.M. and Eskandari, M.H. (2012). Development of latex agglutination test with recombinant NcSAG1 for the rapid detection of antibodies to Neospora caninum in cattle. Veterinary Parasitology, 189(2-4): 211-217.
Morovati, H. and Noaman, V. (2016). Seroepidemiology of Neospora caninum in dairy cattle farms with a history of abortion in Isfahan province, Iran. Journal of Veterinary Sciences and Animal Husbandry, 4(3): 304.
Mosallanejad, B., Razi Jalali, M.H., Hamidinejat, H. and Peyghambari, E. (2018). A serological survey on Neospora caninum infection in wild rats (Rattus rattus) in Ahvaz district, Iran. Journal of Zoonotic Diseases, 3(1): 1-9.
Motamedi, A., Keihani, P. and Momtaz, H. (2020). Molecular detection of Neospora caninum in infected dogs of Isfahan, Iran. Journal of Zoonotic Diseases, 4(1): 35-42.
Namavari, M.M. (2020). Neosporosis in Iran; recent evidences and perspectives. Journal of Zoonotic Diseases, 4(2): 1-24.
Nematollahi, A. and Jafari-Jozani R. (2010). Study on pattern of Neospora caninum tachyzoite proteins by SDS-PAGE and Western blotting in aborted cows. Iranian Journal of Veterinary Research, 11(4): 383-386.
Nematollahi, A., Jaafari, R. and Moghaddam, G. (2011). Seroprevalence of Neospora caninum infection in dairy cattle in Tabriz, Northwest Iran. Iranian Journal of Parasitology, 6(4): 95-98.
Nematollahi, A., Moghaddam, G.H., Jaafari, R., Helan, J.A. and Norouzi, M. (2013). Study on outbreak of Neospora caninum-associated abortion in dairy cows in Tabriz by serological, molecular and histopathologic methods. Asian Pacific Journal of Tropical Medicine, 6(12): 942-946.
Nematollahi, A. (2014). Veterinary Protozology.1st ed., University of Tabriz, pp: 25-45.
Nematollahi, A., Shahbazi, P. and Fakheri, A. (2021). Study on Prevalence rate of Neospora caninum in dogs around Tabriz through fecal and molecular methods. Journal of Veterinary Research, 76(4): 381-388. [In Persian]
Nourollahi Fard, S., Khalili, M. and Aminzadeh, A. (2008). Prevalence of antibodies to Neospora caninum in cattle in Kerman province, South East Iran. Veterinarski Arhiv, 78(3): 253-259.
Norollahifard, S.R., Khalili, M., Fazli, O., Sharifi, H. and Radfar, M.H. (2017). Seroprevalence of Neospora caninum in cattle of Neishabour, Northeast Iran. Slovenian Veterinary Research, 54(1): 5-9.
Noori, M., Rasekh, M., Ganjali, M. and Nourollahi-Fard, S.R. (2019). Seroprevalence of Neospora caninum infection and associated risk factors in cattle of Sistan areas, Southeastern Iran in 2016. Iranian Journal of Parasitology, 14(2): 340-346.
Pouramini, A., Jamshidi, S., Shayan, P., Ebrahimzadeh, E., Namavari, M. and
Shirian, S. (2017). Molecular and serological detection of Neospora caninum in multiple tissues and CSF in asymptomatic infected stray dogs. Iranian Journal of Veterinary Medicine, 11(2): 105-112.
Rafati, N. and Jaafarian, M. (2014). The determination of prevalence of in aborted fetuses in dairy cattle of Shahrekord area, Chaharmahalva Bakhtiari province, by Nested-PCR. Journal of Veterinary Laboratory Research, 6(1): 45-50. [In Persian]
Ranjbarbahadori, S., Motevaselian, A., Bokaie, S. and Yousefi, M. (2010). Serological study of Neospora caninum in aborted dairy cattle in Garmsar, Iran. Comparative Biopathology, 6(2): 249-254. [In Persian]
Razmi, G.R., Mohammadi, G.R., Garrosi, T., Farzaneh, N., Fallah, A.H. and Maleki, M. (2006). Seroepidemiology of Neospora caninum infection in dairy cattle herds in Mashhad area, Iran. Veterinary Parasitology, 135(2): 187-189.
Razmi, G.R., Maleki, M., Farzaneh, N., Garoussi, M.T. and Fallah, A. (2007). First report of Neospora caninum-associated bovine abortion in Mashhad area, Iran. Parasitology Research, 100(4): 755-757.
Razmi, G.R. (2009). Fecal and molecular survey of Neospora caninum in farm and household dogs in Mashhad area, Khorasan province, Iran. Korean Journal of Parasitology, 47(4): 417-420.
Razmi, G.R., Zarea, H. and Naseri, Z. (2010). A survey of Neospora caninum associated bovine abortion in large dairy farms of Mashhad, Iran. Parasitology Research, 106(2): 1419-1423.
Razmi, G.R., Zarae, H., Norbakhsh, M.F. and Naseri, Z. (2013). Estimating the rate of transplacental transmission of Neospora caninum to aborted fetuses in seropositive dams in Mashhad area, Iran. Iranian Journal of Veterinary Medicine, 7(4): 253-256.
Razmi, G. and Barati, M. (2017). Prevalence of Neospora caninum and Toxoplasma gondii antibodies in bulk milk of dairy cattle, Mashhad, Iran. Archive Razi Institute, 72(3): 265-269.
Razmi, G.R. and Naseri, Z. (2017). Molecular detection of Neospora caninum infection in ovine aborted foetuses in the Mashhad area, Iran. Annal Parasitology, 63(1): 45-47.
Reichel, M.P., Ayanegui-Alce´rreca, M.A., Gondim, L.F.P. and Ellis, J.T. (2013). What is the global economic impact of Neospora caninum in cattle—the billion-dollar question? International Journal of Parasitology, 43(2): 133-142.
Sadrebazzaz, A., Haddadzadeh, H., Esmailnia, K., Habibi, G., Vojgani, M. and Hashemifesharaki, R. (2004). Serological prevalence of Neospora caninum in healthy and aborted dairy cattle in Mashhad, Iran. Veterinary Parasitology, 124(3-4): 201-204.
Sadrebazzaz, A., Habibi, G., Haddadzadeh, H. and Ashrafi, J. (2007). Evaluation of bovine abortion associated with Neospora caninum by different diagnostic techniques in Mashhad, Iran. Parasitology Research, 100(6): 1257-1260.
Salehi, N., Haddadzadeh, H.R., Shayan, P., Vojgani, M. and Bolorchi, M. (2010). Serological study of Neospora caninum in pregnant dairy cattle in Tehran, Iran. International Journal of Veterinary Research, 4(2): 113–116.
Sattari, A., Moshiri, F. and Musavi, S. (2011). The seroprevalence of Neospora caninum antibodies in dairy cattle herds in Golestan province, Iran. Journal of Veterinary Microbiology, 7(1): 60-64.
Shakerian, A., Sherafati, R., Rafati, N. and Sharifzadeh, A. (2015). Detection of Neospora caninum in raw milk of cattle in Shahrekord Using PCR. Journal of Tropical Infection Diseases, 20(70): 9-14. [In Persian]
Sharifdini, M., Mohebali, M., Keshavarz, H., Hosseininejad, M., Hajjaran, H., Akhoundi, B., et al. (2011). Neospora caninum and Leishmania infantum co-infection in domestic dogs (Canis familiaris) in Meshkin-shahr district, Northwestern Iran. Journal of Arthropod-Borne Diseases, 1(3): 60-68.
Sharifzadeh, A., Doosti, A. and Dehkordi, P.G. (2012). PCR assay for detection of Neospora caninum in fresh and frozen semen specimens of Iranian bulls. World Applied Sciences Journal, 17(6): 742-749.
Taheri Lak, K., Sadraeii, J. and Dalimi, A. (2017). Prevalence of antibodies to Neospora caninum in milk samples from dairy farms in the Shahriyar. Veterinary Journal (Pajouhesh & Sazandegi) 115(2): 142-146. [In Persian]
Tavanaee, H.R. and Namavari, M. (2017). Evaluation of attenuated variety of Neospora caninum for diagnosis of infection in cattle by agglutination test. Veterinary Journal (Pajouhesh & Sazandegi) 115(4): 153-157. [In Persian]
Varcasia, A., Capelli, G., Ruiu, A., Ladu, M., Scala, A. and Bjorkman, C. (2006). Prevalence of Neospora caninum infection in Sardinian dairy farms (Italy) detected by iscom ELISA on tank bulk milk. Parasitology Research, 98(3): 264-267.
Williams, D.J.L., Hartley, C.S., Björkman, C. and Trees, A.J. (2009). Endogenous and exogenous transplacental transmission of Neospora caninum–how the route of transmission impacts on epidemiology and control of disease. Parasitology, 136(14): 1895-1900.
Wouda, W., Moen, A.R. and Schukken, Y.H. (1998). Abortion risk in progeny of cows after a Neospora caninum epidemic. Theriogenology, 49(7): 1311-1316.
Yakhchali, M., Javadi, S. and Morshedi, A. (2010). Prevalence of antibodies to Neospora caninum in stray dogs of Urmia, Iran. Parasitology Research, 106(6): 1455-1458.
Yakhchali, M., Bahrami, M., Asri-Rezaei, S. and Bokaie, S. (2017). The enzymes and electrolytes profiles in sera of Iranian stray dogs naturally infected with Neospora caninum. Annal Parasitology, 63(1): 63-68.
Youssefi, M.R., Arabkhazaeli, F. and Hassan, A.T.M. (2009). Seroprevalence of Neospora caninum infection in rural and industrial cattle in northern Iran. Iranian Journal of Parasitology, 4(1): 15-18.
Youssefi, M.R, Ebrahimpour, S. and Esfandiari, B. (2010). Survey of Neospora caninum antibody in aborting cattle from three climate regions of Iran. World Applied Sciences Journal, 10(12): 1448-1451.
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Adhami, G., Hoghooghi, R. and Dalimi, A. (2014). The seroepidemiological investigation into Neospora caninum in cattle in Sanandaj, Kordestan Province. Journal of Veterinary Microbiology, 10(2): 83-92. [In Persian]
Alipour, M., Rahimi, E. and Shakerian, A. (2019). Study the prevalence of Neospora caninum in milk of ruminants by molecular method. Journal of Veterinary Microbiology, 15(2): 101-110.
Ansari-Lari, M., Rowshan, A., Jesmani, H., Masoudian, M. and Badkoobeh, M. (2017). Association of Neospora caninum with reproductive performance in dairy cows: a prospective study from Iran. Veterinary Research Forum, 8(2): 109-114.
Binaei, M., Changizi, E. and Staji, H. (2021). A serological study of Neospora caninum infection in dairy cattle in Semnan province, Iran. Journal of Veterinary Research, 76(1): 1-7. [In Persian]
Chryssafidis, A.L., Soares, R.M., Rodrigues, A.A.R., Carvalho, N.A.T. and Gennari, S.M. (2011). Evidence of congenital transmission of Neospora caninumin naturally infected water buffalo (Bubalus bubalis) fetus from Brazil. Parasitology Research, 108(3): 741-743.
Dalimi, A., Sabevarinejad, G., Ghafarifar, F. and Forouzandeh-Moghadam, M. (2014). Molecular detection of Neospora caninum from naturally infected dogs in Lorestan province, West of Iran. Archive Razi Institute, 69(2): 185-190.
Doosti, A., Khamesipour, F., Nekoei, S. and Lutvikadic, I. (2015). Survey for the presence of Neospora caninum in frozen bull’s semen samples by PCR assay. Asian Pacific Journal of Tropical Disease, 5(1): 7-12.
Dubey, J.P. (2003a). Review of Neospora caninum and neosporosis in animals. Korean Journal of Parasitology, 41: 1-16.
Dubey, J.P. (2003b). Neosporiosis in cattle. Journal of Parasitology, 89(1): 42-46.
Dubey, J.P., Sreekumar, C., Knickman, E., Miska, K.B., Vianna, M.C.B. and Kwok, O.C.H. (2004). Biologic, morphologic, and molecular characterization of Neospora caninum isolates from littermate dogs. International Journal of Parasitology, 34(10): 1157-1167.
Dubey, J.P., Schares, G. and Ortega-Mora, L. (2007). Epidemiology and control of neosporosis and Neospora caninum. Clinical Microbiology Reviews, 20(2): 323-367.
Dubey, J.P., Jenkins, M.C., Rajendran, C., Miska, K., Ferreira, L.R., Martins, J., Kwok, O.C.H. and Choudhary, S. (2011a). Gray wolf (Canis lupus) is a natural definitive host for Neospora caninum. Veterinary Parasitology, 181(2-4): 382-387.
Dubey, J.P. and Schares, G. (2011b). Neosporosis in animals—the last five years. Veterinary Parasitology, 180(1-2): 90-108.
Gharedaghi, Y. (2012). Seroprevalence of Neospora caninum in stray dogs of Tabriz Iran. Journal of Animal and Veterinary Advanced, 11(6): 723-726.
Gharekhani, J., Heidari, H. and Akbarein, H. (2012). Seroepidemiology of Neospora caninum in Iranian native and crossbreed cattle: across sectional study. Journal of Veterinary Research, 67(4): 325-329.
Gharekhani, J. and Heidari, H. (2014). Detection of anti-Neospora caninum antibodies in Iranian native cattle. Journal of Advanced Veterinary and Animal Research, 1(4): 228-231.
Gharekhani, J., Yakhchali, M. and Khaltabadi-Farahani, R. (2020). Prevalence and risk factors associated to Neospora caninum (Apicomplexa: Toxoplasmatidae) in pet dogs from Hamadan, West of Iran. Avicenna Journal of Clinical Microbiology Infection, 7(1): 22-26.
Habibi, G.R., Hashemi-Fesharki, R., Sadrebazzaz, A., Bozorgi, S. and Bordbar, N. (2005). Semi-Nested PCR for diagnosis of Neospora caninum infection in cattle. Archive Razi Institue, 59(2): 55-64.
Hadadi, M.R., Sherafati, R., Delavari, M., Arbabi, M., Gilasi, H.R. and Abed, A. (2018). Evaluation of anti-Neospora caninum antibody presence in cow’s milk in Kashan. Feyz: (Journal of Kashan University of Medical Sciences), 22(3): 333-338. [In Persian]
Haddadzadeh, H., Sadrebazzaz, A., Malmasi, A., Ardakani, H.T., Nia, P.K. and Sadreshirazi, N. (2007). Seroprevalence of Neospora caninum infection in dogs from rural and urban environments in Tehran, Iran. Parasitology Research, 101(6): 1563-1565.
Hamidinejat, H., Mosalanejad, B., Avizeh, R., Jalali, R., Hosseini, M., Ghorbanpour, M. and Namavari, M. (2011) .Neospora caninum and Toxoplasma gondii antibody prevalence in Ahvaz feral cats. Iranian.Jundishapur Journal of Microbiology, 4(4): 217-222.
Hashemi Fesharaki, S.R. (2009).Neospora caninum and neosporosis. Pasouk Publication, 1-23.
Heidari, H., Mohammadzadeh, A. and Gharekhani, J. (2014). Seroprevalence of Neospora caninum in slaughtered native cattle in Kurdistan province, Iran. Veterinary Research Forum, 5(1): 69-72.
Hoseini, A., Merat, E., Samani, S., Nezhad, S.S. and Danandeh, R. (2018). Comparison of Neospora caninum infected tissues in aborted fetal bovine by PCR. Journal of Veterinary Research, 73(3): 377-382.
Hosseininejad, M., Hosseini, F., Mahzounieh, M., Nafchi, A.R. and Mosharraf, M. (2010). Seroprevalence of Neospora caninum infection in dogs in Chaharmahal-va-Bakhtiari province, Iran. Comparative Clinical Pathology, 19(3): 269-270.
Hosseininejad, M., Hosseini, F. and Auobei, S. (2018). Seroprevalence of Neospora caninum and Toxoplasma gondii in dogs in Ahwaz, Iran. Veterinary Research and Biological Products, 122(1): 97-100. [In Persian]
Jaafari Joozani, R.A., Asadpour, R., Nematolahi, A. and Hosseininejad, M. (2012). Detection of non-spermatozoal cells of Neospora caninum in fresh semen of naturally infected bulls. Acta Scientiae Veterinariae, 40(2): 1-7.
Kamali, A., Seifi, H.A., Movassaghi, A.R., Razmi, G.R. and Naseri, Z. (2014). Histopathological and molecular study of Neospora caninum infection in bovine aborted fetuses. Asian Pacific Journal of Tropical Biomedicine, 4(12): 990-994.
Khani, M., Arabkhazaeli, F., Hosseini, S.D. and Shayan, P. (2018). Molecular detection of Neospora caninum in aborted fetuses of cattle farms in Arak. Journal of Veterinary Research, 73(4): 457-463. [In Persian]
Khanmohammadi, M. and Fallah, E. (2011). Prevalence of Neospora caninum antibodies in shepherd dogs in Sarab district, EastAzerbaijan province, Iran. African Journal of Microbiology Research, 5(28): 5062-5066.
Khodakaram-Tafti, A., Mansourian, M., Namavari, M. and Hosseini, A. (2012). Immunohistochemical and polymerase chain reaction studies in Neospora caninum experimentally infected broiler chicken embryonated eggs. Veterinary Parasitology, 188(1-2): 10-13.
Malmasi, A., Hosseininejad, M., Haddadzadeh, H., Badii, A. and Bahonar, A. (2007). Serologic study of anti-Neospora caninum antibodies in household dogs and dogs living in dairy and beef cattle farms in Tehran, Iran. Parasitology Research, 100(5): 1143-1145.
Moraveji, M., Hosseini, M., Amrabadi, O., Rahimian, A, Namazi, F. and Namavari, M. (2011). Seroprevalence of Neospora spp. in horses in South of Iran. Tropical Biomedicine, 28(3): 514-517.
Moraveji, M., Hosseini, A., Moghaddar, N., Namavari, M.M. and Eskandari, M.H. (2012). Development of latex agglutination test with recombinant NcSAG1 for the rapid detection of antibodies to Neospora caninum in cattle. Veterinary Parasitology, 189(2-4): 211-217.
Morovati, H. and Noaman, V. (2016). Seroepidemiology of Neospora caninum in dairy cattle farms with a history of abortion in Isfahan province, Iran. Journal of Veterinary Sciences and Animal Husbandry, 4(3): 304.
Mosallanejad, B., Razi Jalali, M.H., Hamidinejat, H. and Peyghambari, E. (2018). A serological survey on Neospora caninum infection in wild rats (Rattus rattus) in Ahvaz district, Iran. Journal of Zoonotic Diseases, 3(1): 1-9.
Motamedi, A., Keihani, P. and Momtaz, H. (2020). Molecular detection of Neospora caninum in infected dogs of Isfahan, Iran. Journal of Zoonotic Diseases, 4(1): 35-42.
Namavari, M.M. (2020). Neosporosis in Iran; recent evidences and perspectives. Journal of Zoonotic Diseases, 4(2): 1-24.
Nematollahi, A. and Jafari-Jozani R. (2010). Study on pattern of Neospora caninum tachyzoite proteins by SDS-PAGE and Western blotting in aborted cows. Iranian Journal of Veterinary Research, 11(4): 383-386.
Nematollahi, A., Jaafari, R. and Moghaddam, G. (2011). Seroprevalence of Neospora caninum infection in dairy cattle in Tabriz, Northwest Iran. Iranian Journal of Parasitology, 6(4): 95-98.
Nematollahi, A., Moghaddam, G.H., Jaafari, R., Helan, J.A. and Norouzi, M. (2013). Study on outbreak of Neospora caninum-associated abortion in dairy cows in Tabriz by serological, molecular and histopathologic methods. Asian Pacific Journal of Tropical Medicine, 6(12): 942-946.
Nematollahi, A. (2014). Veterinary Protozology.1st ed., University of Tabriz, pp: 25-45.
Nematollahi, A., Shahbazi, P. and Fakheri, A. (2021). Study on Prevalence rate of Neospora caninum in dogs around Tabriz through fecal and molecular methods. Journal of Veterinary Research, 76(4): 381-388. [In Persian]
Nourollahi Fard, S., Khalili, M. and Aminzadeh, A. (2008). Prevalence of antibodies to Neospora caninum in cattle in Kerman province, South East Iran. Veterinarski Arhiv, 78(3): 253-259.
Norollahifard, S.R., Khalili, M., Fazli, O., Sharifi, H. and Radfar, M.H. (2017). Seroprevalence of Neospora caninum in cattle of Neishabour, Northeast Iran. Slovenian Veterinary Research, 54(1): 5-9.
Noori, M., Rasekh, M., Ganjali, M. and Nourollahi-Fard, S.R. (2019). Seroprevalence of Neospora caninum infection and associated risk factors in cattle of Sistan areas, Southeastern Iran in 2016. Iranian Journal of Parasitology, 14(2): 340-346.
Pouramini, A., Jamshidi, S., Shayan, P., Ebrahimzadeh, E., Namavari, M. and
Shirian, S. (2017). Molecular and serological detection of Neospora caninum in multiple tissues and CSF in asymptomatic infected stray dogs. Iranian Journal of Veterinary Medicine, 11(2): 105-112.
Rafati, N. and Jaafarian, M. (2014). The determination of prevalence of in aborted fetuses in dairy cattle of Shahrekord area, Chaharmahalva Bakhtiari province, by Nested-PCR. Journal of Veterinary Laboratory Research, 6(1): 45-50. [In Persian]
Ranjbarbahadori, S., Motevaselian, A., Bokaie, S. and Yousefi, M. (2010). Serological study of Neospora caninum in aborted dairy cattle in Garmsar, Iran. Comparative Biopathology, 6(2): 249-254. [In Persian]
Razmi, G.R., Mohammadi, G.R., Garrosi, T., Farzaneh, N., Fallah, A.H. and Maleki, M. (2006). Seroepidemiology of Neospora caninum infection in dairy cattle herds in Mashhad area, Iran. Veterinary Parasitology, 135(2): 187-189.
Razmi, G.R., Maleki, M., Farzaneh, N., Garoussi, M.T. and Fallah, A. (2007). First report of Neospora caninum-associated bovine abortion in Mashhad area, Iran. Parasitology Research, 100(4): 755-757.
Razmi, G.R. (2009). Fecal and molecular survey of Neospora caninum in farm and household dogs in Mashhad area, Khorasan province, Iran. Korean Journal of Parasitology, 47(4): 417-420.
Razmi, G.R., Zarea, H. and Naseri, Z. (2010). A survey of Neospora caninum associated bovine abortion in large dairy farms of Mashhad, Iran. Parasitology Research, 106(2): 1419-1423.
Razmi, G.R., Zarae, H., Norbakhsh, M.F. and Naseri, Z. (2013). Estimating the rate of transplacental transmission of Neospora caninum to aborted fetuses in seropositive dams in Mashhad area, Iran. Iranian Journal of Veterinary Medicine, 7(4): 253-256.
Razmi, G. and Barati, M. (2017). Prevalence of Neospora caninum and Toxoplasma gondii antibodies in bulk milk of dairy cattle, Mashhad, Iran. Archive Razi Institute, 72(3): 265-269.
Razmi, G.R. and Naseri, Z. (2017). Molecular detection of Neospora caninum infection in ovine aborted foetuses in the Mashhad area, Iran. Annal Parasitology, 63(1): 45-47.
Reichel, M.P., Ayanegui-Alce´rreca, M.A., Gondim, L.F.P. and Ellis, J.T. (2013). What is the global economic impact of Neospora caninum in cattle—the billion-dollar question? International Journal of Parasitology, 43(2): 133-142.
Sadrebazzaz, A., Haddadzadeh, H., Esmailnia, K., Habibi, G., Vojgani, M. and Hashemifesharaki, R. (2004). Serological prevalence of Neospora caninum in healthy and aborted dairy cattle in Mashhad, Iran. Veterinary Parasitology, 124(3-4): 201-204.
Sadrebazzaz, A., Habibi, G., Haddadzadeh, H. and Ashrafi, J. (2007). Evaluation of bovine abortion associated with Neospora caninum by different diagnostic techniques in Mashhad, Iran. Parasitology Research, 100(6): 1257-1260.
Salehi, N., Haddadzadeh, H.R., Shayan, P., Vojgani, M. and Bolorchi, M. (2010). Serological study of Neospora caninum in pregnant dairy cattle in Tehran, Iran. International Journal of Veterinary Research, 4(2): 113–116.
Sattari, A., Moshiri, F. and Musavi, S. (2011). The seroprevalence of Neospora caninum antibodies in dairy cattle herds in Golestan province, Iran. Journal of Veterinary Microbiology, 7(1): 60-64.
Shakerian, A., Sherafati, R., Rafati, N. and Sharifzadeh, A. (2015). Detection of Neospora caninum in raw milk of cattle in Shahrekord Using PCR. Journal of Tropical Infection Diseases, 20(70): 9-14. [In Persian]
Sharifdini, M., Mohebali, M., Keshavarz, H., Hosseininejad, M., Hajjaran, H., Akhoundi, B., et al. (2011). Neospora caninum and Leishmania infantum co-infection in domestic dogs (Canis familiaris) in Meshkin-shahr district, Northwestern Iran. Journal of Arthropod-Borne Diseases, 1(3): 60-68.
Sharifzadeh, A., Doosti, A. and Dehkordi, P.G. (2012). PCR assay for detection of Neospora caninum in fresh and frozen semen specimens of Iranian bulls. World Applied Sciences Journal, 17(6): 742-749.
Taheri Lak, K., Sadraeii, J. and Dalimi, A. (2017). Prevalence of antibodies to Neospora caninum in milk samples from dairy farms in the Shahriyar. Veterinary Journal (Pajouhesh & Sazandegi) 115(2): 142-146. [In Persian]
Tavanaee, H.R. and Namavari, M. (2017). Evaluation of attenuated variety of Neospora caninum for diagnosis of infection in cattle by agglutination test. Veterinary Journal (Pajouhesh & Sazandegi) 115(4): 153-157. [In Persian]
Varcasia, A., Capelli, G., Ruiu, A., Ladu, M., Scala, A. and Bjorkman, C. (2006). Prevalence of Neospora caninum infection in Sardinian dairy farms (Italy) detected by iscom ELISA on tank bulk milk. Parasitology Research, 98(3): 264-267.
Williams, D.J.L., Hartley, C.S., Björkman, C. and Trees, A.J. (2009). Endogenous and exogenous transplacental transmission of Neospora caninum–how the route of transmission impacts on epidemiology and control of disease. Parasitology, 136(14): 1895-1900.
Wouda, W., Moen, A.R. and Schukken, Y.H. (1998). Abortion risk in progeny of cows after a Neospora caninum epidemic. Theriogenology, 49(7): 1311-1316.
Yakhchali, M., Javadi, S. and Morshedi, A. (2010). Prevalence of antibodies to Neospora caninum in stray dogs of Urmia, Iran. Parasitology Research, 106(6): 1455-1458.
Yakhchali, M., Bahrami, M., Asri-Rezaei, S. and Bokaie, S. (2017). The enzymes and electrolytes profiles in sera of Iranian stray dogs naturally infected with Neospora caninum. Annal Parasitology, 63(1): 63-68.
Youssefi, M.R., Arabkhazaeli, F. and Hassan, A.T.M. (2009). Seroprevalence of Neospora caninum infection in rural and industrial cattle in northern Iran. Iranian Journal of Parasitology, 4(1): 15-18.
Youssefi, M.R, Ebrahimpour, S. and Esfandiari, B. (2010). Survey of Neospora caninum antibody in aborting cattle from three climate regions of Iran. World Applied Sciences Journal, 10(12): 1448-1451.
Shirian, S. (2017). Molecular and serological detection of Neospora caninum in multiple tissues and CSF in asymptomatic infected stray dogs. Iranian Journal of Veterinary Medicine, 11(2): 105-112.
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Shirian, S. (2017). Molecular and serological detection of Neospora caninum in multiple tissues and CSF in asymptomatic infected stray dogs. Iranian Journal of Veterinary Medicine, 11(2): 105-112.