Protective effect of nano selenium on renal ischemia/reperfusion injury in male rats

Subject Areas : Veterinary Clinical Pathology

Farzaneh Sadeghmanesh ¹ , Akram Eidi ² , Pejman Mortazavi ³ , Shahrbanoo Oryan ⁴

1 - PhD Graduate, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2 - Professor, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
3 - Associate Professor, Department of Pathology, Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
4 - Professor, Department of Biology, Kharazmi University, Tehran, Iran.

Received: 2023-08-20 Accepted : 2024-01-20 Published : 2024-05-19

Keywords: Ischemia/Reperfusion, Kidney, Rat, Selenium nanoparticle.,

Abstract :

Selenium is an important element in nutrition and an essential part of several proteins with catalytic and structural properties. Selenium nanoparticles have a higher effect and less toxicity than conventional selenium. The aim of this study was to demonstrate the strong role of nano-selenium in protecting against renal ischemia/reperfusion (I/R) injury in a male rat model. For this purpose, I/R injury was caused by occlusion of the left renal artery for 20 minutes. A total of 54 male Wistar rats were randomly divided into healthy and sham control groups, three healthy experimental groups receiving selenium nanoparticles at doses of 0.25, 0.5 and 1 mg/kg, I/R control group and three groups of I/R receiving selenium nanoparticles at previously mentioned doses (n=6). Thirty days after administration, the animals were euthanized for biochemical and histopathological evaluation and the data were statistically analyzed (p˂0.05). Selenium nanoparticles in I/R groups significantly decreased serum urea and creatinine levels (p˂0.001) and significantly increased the antioxidant parameters of catalase, glutathione peroxidase and superoxide dismutase and significantly decreased the level of malondialdehyde (p˂0.001) and improved renal tissue damage (p˂0.001) in comparison to other groups. This study showed that selenium nanoparticles have protective effects against damage caused by renal ischemic reperfusion, and this protective effect can be due to their antioxidant properties in improving injuries caused by free radicals in the ischemic reperfusion process, thereby reducing oxidative stress and improving renal tissue structure.