The effect of hydro-alcoholic extract of Melissa officinalis on left ventricular fractional shortening in rats
Subject Areas : Veterinary Clinical PathologyRamin Bahramnejad Heris 1 , Gh. Assadnassab, 2 *
1 - .V.M Graduate, Faculty of Veterinary Medicine, Tabriz Medical Sciences, Islamic Azad University, Tabriz, Iran.
2 - Associate Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine, Tabriz Medical Sciences, Islamic Azad University, Tabriz, Iran.
Keywords: Echocardiography, Hydroalcoholic extract, Left ventricular Fractional shortening, Melissa officinalis, Rat.,
Abstract :
Melissa officinalis is a medicinal plant. In this study, the effect of hydro-alcoholic extract of Melissa officinalis on left ventricular fractional shortening was determined in the rat model. To conduct this study, standard echocardiography was performed in all 40 male Wistar rats, and fractional shortening was determined. The animals were randomly divided into four groups. The first group was the control group, and the second to fourth groups were administered the intra-peritoneal injection of Melissa officinalis extract daily for 21 days with doses of 100, 200, and 400 mg/kg respectively. The animals underwent echocardiography on days 7, 14, and 21 after administration, and the fractional shortening was calculated. The fractional shortening in the fourth group showed the greatest increase, with an average of 34.74±1.8%, and a significant difference compared to the other groups on the final day (p<0.05). However, within the fourth group, the means of the fractional shortening data on the seventh and fourteenth day did not show significant changes (p<0.05). This study shows the increase of fractional shortening following the administration of the extract in different doses and with the passage of time. However, this increase can only be seen at the highest dose and even after the longest time of administration, which may be caused by the effect of this particular dose under study conditions.