Investigation of the magnetic field effects on germination growth and peroxidase enzyme activity in Black locust seeds

Subject Areas :

Seyed yousef Torabian ¹ (Assistant Professor, Department of Forestry, Lahijan Branch, Islamic Azad University, Lahijan, Iran.)
Vahid Hemmati ² (Associate Professor, Department of Forestry, Lahijan Branch, Islamic Azad University, Lahijan, Iran.)
Seyed Armin Hashemi ³ (Assistant Professor, Department of Forestry, Lahijan Branch, Islamic Azad University, Lahijan, Iran.)

Received: 2022-08-10 Accepted : 2022-11-20 Published : 2022-08-23

Keywords: Magnetic field, Peroxidase enzyme, Black locust, Germination, Millitesla,

Abstract :

This research was conducted with the aim of finding the magnetic field effect on changes in peroxidase enzyme activity, germination percentage, stem length and root growth of black locust seeds. The seed cultivation was done in 3 treatments (control, 4 millitesla and 8 millitesla) with 3 replications. 100 seeds were planted in each of the treatment containers, the sprouts were placed in the magnetic field for fifteen minutes in ten days and then the sprouts were counted. The rate of growth of root and stem was measured by a ruler and after that extraction was done. The obtained extract was placed in a centrifuge device and the peroxidase enzyme activity was calculated by a spectrophotometer in the light spectrum of 420 nm. LSD test was used to analyze the data. The results showed that there was a significant difference between the samples affected by the magnetic field regarding all four factors (percentage of germination, root and stem lengths (cm) and the enzyme activity level) with the control sample (p<0 /05). This confirmed that the buds created under the influence of the magnetic field had a higher physiological capacity. However, no significant difference was observed between the magnetic field of 4 and 8 millitesla (p>0.05). It is recommended for the germination of black locust species, which is one of the resistant and very important species in terms of physiological ability to create a green space, a magnetic field of 4 millitesla should be used.