Dominant bacterial flora of gut in Rainbow trout and probable important viewpoint probiotic

Subject Areas : New Technologies in Aquaculture Development

علیرضا Mobarhanfard ¹

1 -

Received: 2022-10-09 Accepted : 2022-10-09 Published : 2022-05-22

Keywords: Probiotic, Bacterial Flora, Keywords: Rainbow trout,

Abstract :

AbstractThis research conducted for recognition of dominant bacterial flora of rainbow trout gut. 20 fingerlings of trout with average weight of 2gr, prepared by Isfahan Fisheries Authority in last winter. The stock of fish reared isolation tank, and during four months of rearing period, neither any disinfections nor antibiotic agents have been used. The temperature of farming tank maintained at the range of 10 to 16. After this period, the stock of fish pocked in ewo layer bags and transported at night time to lobaratory of science and Research Unit of Azad University in Tehran. All samples of fish arrived at healthy condition to laboratory. The average weight of fish measured as 30 10g, and total length of 8-12 cm. After word, the fish disinfected by 70% alcohol, and them by the strile apparatus the gut have been removed for primary and subtractive culture. The media used for culture was N.A, M.A, TSI, SIM, and recognition of positive or negative grams carried by staining. In addition, biochemical reagents like methyl red, oxidase – catalase test, sugar fermentation test, gelatin hydrolysis test and starch hydrolysis test for final recognition of bacteria and abundance ratio is carried out. Edwardzillata sp.(8%), itrobacrer sp. (10%), Shewanella sp.(30%), Haemophilus sp.(2%), Plesiomonas sp.(25%), Aero,onas sp.(25%) Dominant bacteria of gut flora in rainbow trout showed following species; Shewanella sp., Plesioonas sp. and Aeromonaa sp.