Evaluation of cellular and humoral immunity during Sinopharm vaccination
Subject Areas : Immunology
Keywords: Coronavirus, COVID-19, lymphocytes, Vaccine, Sinopharm,
Abstract :
Background and purpose: In this research, the immunological events that occurred during vaccination with COVID-19 as well as its cellular and molecular mechanisms were studied. The purpose of this study is to investigate cellular immunity and lymphocyte activating genes, as well as to identify changes in Th lymphocyte surface markers during the period of COVID-19 vaccination with Sinopharm vaccine. Materials and methods: In this study, blood was taken from 60 healthy people who had not been infected with corona in the past , and CD3 and CD4 changes during vaccination were measured. Also, IgM and IgG antibody levels were evaluated, and real time QPCR method was used to measure the expression level of IFN-α and IFN-γ genes. Results: The results showed that after the injection of the first dose of Sinopharm vaccine, IgM and IgG increased significantly in 65% and 60% of people, respectively. After the second dose, a significant increase of 80% was observed for both antibodies in the participants. The percentage of TCD3+ lymphocytes decreased (25%), remained unchanged (15%) and increased (60%) after the first dose of vaccine. This frequency after the second dose was 15, 15 and 70% respectively. After the first dose of vaccine, there was a 20% decrease in the percentage of TCD4+ lymphocytes, no change in 30% and a significant increase in 50%. After the second dose, these values were determined as 20, 20 and 60%, respectively. Conclusion: The results of the Sinopharm vaccine trials show the creation of strong and specialized neutralizing antibodies in most persons. Additionally, the booster dose of the vaccine ensures a high stimulation of memory cells.
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