Inspection of the antibody titer against Mannheimia haemolytica with different doses of immunogen in sheep for the first time in Iran
Subject Areas : Immunology
Keywords: Mannheimia haemolytica, Inactivated immunogen, Sheep, Elisa. ,
Abstract :
Background & Objectives: Mannheimia haemolytica is one of the main bacterial species involved in lung diseases in ruminants. This bacterium is a part of normal flora in upper respiratory tract of these animals. M. haemolytica is an opportunistic microorganism, gaining access to the lungs when host defenses are compromised by stress or infection with respiratory viruses or mycoplasma. Antibiotics are candidates for treatment of Mannheimiosis but the universal growth of antibiotic resistance reduces their effectiveness. Therefore, vaccines are used broadly to control this disease while the low durability of existing vaccines makes to need vaccine progress. The objective of this study was to prepare inactivated immunogen of M. haemolytica and to define the suitable dose for immunization of sheep. Material & methods: The polymerase chain reaction technique was used for identification of M. haemolytica. Formalin-inactivated immunogen adjuvanted with aluminum hydroxide and injected subcutaneously to sheep in different doses of 0.5, 1, 1.5 and 2 ml. Results: Elisa results advocates long-term humoral immunity in studied animals and 1 ml seems to be sufficient dose for immunization of sheep. Conclusion: Due to the lack of inactivated M. haemolytica vaccine in Iran, the results of this research are important in controlling M. haemolytica infection and provide useful information for making a suitable vaccine to prevent pneumonic pasteurellosis in Iran.
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