Evaluation of the biodegradation of phenanthrene by Bacillus Mojavensis isolated from the contaminated soils of Darkhovin oil regionEvaluation of the biodegradation of phenanthrene by Bacillus Mojavensis isolated from the contaminated soils of Darkhovin oil region

Subject Areas : Applied Microbiology

maryam abbasi ¹ , Farshid Kafilzadeh ² , Azar Sabokbar ³ , Azam Haddadi ⁴

1 -
2 -
3 -
4 - Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran

Received: 2023-08-07 Accepted : 2023-10-29 Published : 2024-01-25

Keywords: Bioremediation, Phenanthrene, Bacillus mojavensis, Optimization,

Abstract :

Background and Objectives: Bioremediation is one of the methods of cleaning the environment from petroleum compounds. The aim of this research was to identify a native phenanthrene-degrading bacterium and to investigate the optimal growth conditions for biodegradation.

Materials and Methods: Sampling was carried out from petroleum-contaminated soils in the Darkhovin oil field, Iran. After isolation and primary identification of the bacteria, the specific bacterium was selected and identified. Then, after initial examination, the amount of biosurfactant produced by the bacterium was measured using a tensiometer. The effect of temperature, time, pH, nitrate, and sodium phosphate factors at 3 different levels was investigated using the Taguchi method, and the selected bacterium was placed under the designed conditions. The amount of phenanthrene degradation was examined using GC-MS, and the obtained results were analyzed. Results: Among the isolates, Bacillus mojavensis had a higher ability to degrade phenanthrene. Evaluation of the presence of biosurfactant in this bacterium showed a value of 47 mN/m .The results of analyzing the optimal conditions of Bacillus mojavensis for biodegradation showed a temperature of 45 °C, pH 7, nitrate 1mg/mL , sodium phosphate 0.3 mg/mL 0.3 , and a time of 5 days, under which conditions 55% of phenanthrene was degraded.

Conclusion: The results of this study showed that, considering the presence of native bacteria capable of degrading phenanthrene in contaminated soils, and determining the level of nutrients, environmental factors, and other effective factors in biodegradation, it is an effective strategy to increase the rate of phenanthrene biodegradation.

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