Frequency of vanA & vanB genes in vancomycin-resistant enterococci isolated from clinical specimens at Shahid Mohammadi hospitals Bandar Abbass
Subject Areas : Bacteriology
Zeinab Ghalandarzade Daryaii
1
(کارشناسی ارشد، دانشگاه آزاد اسلامی، واحد جهرم، گروه میکروبیولوژی)
Sedighe Javadpour
2
(دانشیار، مرکز تحقیقات پزشکی مولکولی، دانشگاه علوم پزشکی هرمزگان)
Mohammad Kargar
3
(دانشیار، دانشگاه آزاد اسلامی، واحد جهرم، گروه میکروبیولوژی)
Keywords: E-test, Vancomycin resistant enterococci, vanA gene, vanB gene,
Abstract :
Background and Objectives: Enterococci are normal flora of alimentary tract of humans and many other mammals. Recently vancomycin resistant enterococci (VRE) have emerged as an increasingly important cause of nosocomial infections all around the world. The aims of this study were to determine frequency and antibiogram patterns of enterococci to vancomycin and also to detect the corresponding genes (vanA and vanB) in clinical isolates. Materials and Methods: This cross–sectional study was carried out on 54 enterococci strains isolated from clinical specimens (urine, wound, blood, …) obtained from the patients hospitalized in Shahid Mohammadi hospital, Bandar Abbass. RapID STR System Kit was used to identify species of isolated enterococci. Antibiogram pattern was examined by Kirby-Bauer disk diffusion method and CLSI criteria. MIC of vancomycin was determined by E-Test. Multiplex PCR and specific primers was used to detect vanA and vanB genes. Results: 38 out of 54 isolates (70.4%) of enterococci belonged to E. faecalis while E, feacium and and E. hirea consist only and 10 (18.5%) and 3 (5.55%) of the isolates, respectively. Furthermore, one (1.85%) of each E.mundtii, E.durans and E.avium were isolated from these samples. These strains were mostly resistant to gentamicin and cephalexin while showed lowest antibiotic resistance to linezolid. 13 (24.10%) of the isolates were resistant to vancomycin. Van A and Van B genes were found in 69.23% and 15.38% of VRE strains, respectively. Two strains (16.38%) harbored both genes. Conclusion: According to the extensive frequency of vancomycin resistant enterococci, application of precautionary and management procedures are highly required. Furthermore, it is recommended to carry out the antibiogram assay for each patient, before the treatment process, in order to prescribe an appropriate antibiotic.
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