In silico analysis of pMGA1.2 protein of Mycoplasma gallisepticum in vaccine and pathogenic strains

Insilico analysis for pMGA1.2 protein in Mycoplasma gallisepticum strains

Subject Areas : Bacteriology

Farzaneh Pourkarimi Fatideh ¹ , Majid Esmaelizad ² , Mohammad Kargar ³ , Majid Tebianian ⁴ , Farshid Kafilzadeh ⁵

1 - Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran.
2 - Research and Development Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Alborz, Iran
3 - Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran
4 - Department of Immunology, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
5 - Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran

Received: 2023-04-28 Accepted : 2023-07-11 Published : 2023-10-02

Keywords: pMGA1.2, Mycoplasma gallisepticum, In silico.,

Abstract :

Background & Objectives: Mycoplasma gallisepticum, the pathogen responsible for chronic respiratory disease in chickens, is the most economically important species of Mycoplasma that causing tremendous economic losses worldwide. The most abundant membrane proteins in M. gallisepticum are pMGA, lipoproteins of about 67 kDa. The pMGA family genes have an extraordinary potential for diversifying antigenic structure on the surface of Mycoplasma gallisepticum cells. The aim of this study was to compare the pMGA protein patterns between different strains and hosts of Mycoplasma gallisepticum. Material & Methods: All Mycoplasma gallisepticum full genomes available in GenBank till January 2020 were considered and pMGA1.2 sequences were identified, grouped and coded. pMGA1.2 protein with a chain of 650 amino acids between two different hosts (poultry and house finch) was studied by bioinformatics software in all Mycoplasma gallisepticum full genomes. Results: pMGA1.2 gene among different strains of Mycoplasma gallisepticum showed five major groups with more than 10 percent divergence. Based on multiple sequence alignment, a specific pattern was identified in house finch isolates. Interestingly, two specific motifs 480DNQNVSNQ487 and 639SSNVSSPSY647 were found in the pMGA1.2 of TS-11 strain, which can probably be used as markers to identify and differentiate this vaccine strain from pathogenic Mycoplasma gallisepticum. Conclusion: This study showed that pMGA1.2 protein have some B-cell epitope antigenic regions that are conserved among all isolates and might be applicable to design serological test for detection antibody against Mycoplasma gallisepticum.

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