Isolation of orange orchards rhizosphere Bacillus cereus by the ability of α-amylase secretion

Subject Areas : Bacteriology

Mahnaz Ramezani ¹ (M.Sc., Department of Biology, Science and Research branch, Islamic Azad University, Kerman, Iran)
Ali Riahi-Madvar ² (2Assistant Professor, Department of Biotechnology, Institute of Science and High Technology and  Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.)
Mooj Khaleghi ³ (Assistant Professor, Department of Biology, Faculty of Sciences, Shahid Bahonar University of Kerman, Iran.)

Received: 2012-08-31 Accepted : 2013-04-24 Published : 2013-06-01

Keywords: 16S rRNA, Bacillus cereus, &alpha, -amylase, Rhizospher,

Abstract :

Background and Objectives: Rhizosphere bacteria assist growth of plants via dissolving phosphate, production of special compounds such as phytohormones and release of hydrolytic enzymes such as a-amylase and kitinase. This study aimed to isolate and to identify a a-amylase producing bacillus in rhizosphere of orange orchards and to investigate microbial activity to enzyme production in presence of different source of carbon ranges. Materials and Methods: This study was conducted in order to isolation of a-amylase bacilli from rhizosphere of orange orchards located at Kerman province, Iran. After sample collection, bacteria were isolated by growing on starch agar. Several molecular (based on 16SrRNA) and biochemical methods were used to identify bacterial species. The starch rapid hydrolysis test was used to investigate amylase activity of the isolates. Additionally, the enzyme production level was studied in the presence of several carbon sources include glucose, fructose and starch ranges. Results: Molecular and biochemical analysis showed that isolated bacterium is a strain of Bacillus cereus named as Bacillus cereus MR-R3 and recorded in GeneBank under accession number of KC306945.1. Moreover, results showed that starch and glucose have the highest positive effects on alpha amylase production in the presence of 0.5 g/l and fructose have the highest effect in the presence of 0.25 g/l. Conclusion: Isolation and identification of orange orchards rhizospher-derived bacillus species considering their ability to produce a-amylase and phosphate solvation showed that the presence of this species in this region is very important. Moreover, increase in production of the enzyme after treatment with different carbon sources can be related to their gene expression induction effects.

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