The prevalence of plasmid genes spvB, spvC and spvR in Salmonella enteritidis isolated from poultry industry in Chaharmahal va Bakhtiari province

Subject Areas : Bacteriology

masoume daruoshi ¹ , Abbas Doosti ² , Mohammad Kargar ³

1 - MS.c., Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Jahrom branch, Jahrom, Iran.
2 - Associate Professor, Biotechnology Research Center, Shahrekord branch., Islamic Azad University, Shahrekord, Iran.
3 - Associate Professor, Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Jahrom branch, Jahrom, Iran.

Received: 2014-05-21 Accepted : 2014-07-13 Published : 2015-01-01

Keywords: Poultry, Salmonella enteritidis, Polymerase chain reaction, spvR, spvBC,

Abstract :

Background & Objectives: Salmonellosis is a one of zoonotic diseases and Salmonella enterica is the most frequent agent of this disease. Some serotypes of Salmonella sp. harbour a plasmid which contains spv operon on it. This operon consists of five genes, namely spvRABCD. The aim of this study was to determine frequency of plasmid genes spvB, spvC and spvR in Salmonellaisolated from poultry industry in Chaharmahal va Bakhtiari province. Materials & Methods: This cross – sectional study was carried out on 305 stool samples  obtained from poultries located at Charmahal va Bakhtiari province. Following identification of Salmonella based on routine biochemical tests. Polymerase chain reaction (PCR) assay was used for distinguish the strains and to determine the prevalence of the gene spvB, spvC and spvR. Results: Salmonella were identified in 160 (45.52%) samples, among them 94 cases (75.58%) were identified as Salmonella enteritidis after sefA amplification test. The prevalence of the genes spvB, spvC and spvR were 7.45, 60.76 and 14.69 %, respectively. Conclusion: Due to the prevalence of Salmonella in poultries and to limitation of biochemical and serological tests, employment of molecular tests are very critical for identification of strains of Salmonella enterica and for distinguishing from other strains all around the country. Furthermore, the high prevalence of the plasmid genes involved in systemic infections can be reduced using Antibiotics.