Evaluation of adherence of probiotic Lactobacilus casei to Hep2– cell line
Subject Areas : Applied MicrobiologyZeinab Takalloo 1 , Mahdi Goudarzvand 2 , Zohreh Khodaii 3
1 - MS.c., Department of Biology, Karaj branch, Islamic Azad University, Karaj, Iran.
2 - Assistant Professor, Department of Physiology, Faculty of Medicine , Alborz University of Medical Sciences, Karaj, Iran.
3 - Assistant Professor, Department of Biochemistry, Genetics and Nutrition, Faculty of Medicine, Alborz University of Medical Sciences, Karaj, Iran.
Keywords: Probiotics, Lactobacillus casei, Adhesion, HEp-2 cells,
Abstract :
The first step to choose a strain as probiotic is capability for the bacteria to adhere to mucosal adhesion due to increase the prevention of the adherence of pathogenic bacteria, and the secretion of antimicrobial agents, which produce an appropriate condition of microbial growth. This study was aimed to evaluate the adherence ability of Lactobacilus casei to Hep-2 cell line. First, the HEp-2 cells were grown in RPMI medium containing fetal calf serum and antibiotics. The defined numbers of probiotic bacteria grown in MRS broth were added to the HEp-2 cell. Then, the cells were washed after attachment of bacteria to the plates. Thereafter, the cells were stained and fixed with histological methods. The adhesion ability of bacteria and the pattern of adhesion was observed under optical microscope. Our results showed that L. casei was able to adhere to oro- pharyngeal cell line. Also, the optimum time to test the ability was 3 hours. These results indicated that the probiotic bacterium L. casei is suitable for application in health products. Based on these results, we recommend application of L. casei in oral hygiene products.
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1. Meurman JH, Stamatova I. Probiotics: contributions to oral health. Oral Dis. 2007; 13(5): 443-451.
2. Vanderpool C, Yan F, Polk D B. Mechanisms of probiotic action: implications for therapeutic applications in inflammatory bowel diseases. Inflamm Bowel Dis. 2008; 1(4): 1585-1596.
3. Bonifait L, Chandad F, Grenier D. Probiotics for oral health: myth or reality? J Can Dent Assoc. 2009; 75(8): 585-590.
4. Twetman S, Stecksen-Blicks C. Probiotics and oral health effects in children. Int J Paediatr Dent. 2008; 18(1): 3-10.
5. Saha S, Tomaro- Duchesneau C,Tabrizian M, Prakash S. Probiotics as oral health biotherapeutics. 2012; 12(9): 1207-1220.
6. Hatakka K, Ahola AJ, Yli H, Richardson M. Probiotics reduce the prevalence of oral Candida in the elderly– a randomized controlled trial. J Dent Res. 2007; 8(6):125-130.
7. Haeri A, Khodaii Z, Ghaderian SMH. Comparison of adherence patterns of a selection of probiotic bacteria to Caco-2, Hep-2 and T84 cell lines. Ann Microbiol. 2012; 62(1): 339-344.
8. Kodama Y, Sasaki M, Shimoyama Y, Tajika S, Kimura S. Adhesion mechanism of Streptococcus anginosus to mucosal epithelial cells. Clin Microbiol Vienna, Austria. 2010; 4(3): 10-13.
9. Tuomola, EM , Salminen SJ. Adhesion of some probiotic and dairy Lactobacillus strains to Caco-2 cell cultures. Int J Food Microbiol. 1998; 41(1): 45-51.
10. Jacobsen CN. Screening of probiotic activities of forty-seven strains of Lactobacillus spp. by in vitro techniques and evaluation of the colonization ability of five selected strains in humans. Appl Environ Microbiol. 1999; 65(11): 4949-4959.
11. Boudeau J. Inhibitory effect of probiotic Escherichia coli strain Nissle 1917 on adhesion to and invasion of intestinal epithelial cells by adherent-invasive E. coli strains isolated from patients with Crohn's disease. Aliment Pharmacol Ther. 2003; 18(1): 45-56.
12. Haukioja A, Yli –Knuuttila H, Liomaranta V. Oral adhesion and survival of probiotic and other lactobacilli and bifidobacteria in vitro. Oral Micorbiol Immunol. 2006; 2(1): 326-332.