Isolation and identification Xanthomonas strain from leaves of lemon trees and evaluation of production of Xanthan gum

Subject Areas : Environmental Microbiology

Saba Amiri Kojuri ¹ , Zoheir Heshmatipour ² , Esmaeil Ghorbanalinejad ³

1 - MS.c., Department of Microbiology, Tonekabon branch, Islamic Azad University, Tonekabon, Iran.
2 - Assistant Professor, Department of Microbiology, Tonekabon branch, Islamic Azad University, Tonekabon, Iran.
3 - Lecturer, Department of Microbiology, Tonekabon branch, Islamic Azad University, Tonekabon, Iran.

Received: 2013-07-20 Accepted : 2013-10-04 Published : 2015-01-01

Keywords: 16S rRNA, Xanthomonas, Xanthan gum, Lime,

Abstract :

Background & Objectives: Xanthan gum is a Heteropolysaccharide at the external cell of Xanthomonas, which acts as toxin for other plants. This study aimed to isolate and identify the Xanthomonas strain from leaves of lemon trees and to evaluate its ability in production of Xanthan gum. Materials & Methods: This cross-sectional study was performed on the leaves of lemon trees infected to bacterial canker. The strain was isolated on YDC medium. The morphological characteristics colonies were compared with the colonies of Xanthomonas  campestris PTCC 1473. Xanthan gum was produced in especial medium. A 16S rRNA procedure used to confirm the identity of strains. Results: In this study a new strain of Xanthomonas campestris was isolated and was called as saba.ton. This strain was recorded in the gene bank with access number KF706548.1. The amount of Xanthan production was obtained 0.081 to 1.5 g/100 ml. Conclusion: In this study a new strain of Xanthomonas campestris was isolated. This strain was identified as an Iranian local strain which is potentially able to produce Xanthan gum without any optimization.