Analysis of zinc resistance gene in zinc and zinc oxide nanoparticles resistant Pseudomonas stutzeri SEE-1 isolated from soil

Subject Areas : Environmental Microbiology

Shahla Soltani Nezhad ¹ , Mohammad Rabbani Khorasgani ² , Giti Emtiazi ³

1 - Assistant Professor, Department of Biology, Islamic Azad University, Jiroft branch, Jiroft, Iran.
2 - Associate Professor, Department of Microbiology, Faculty of Sciences, University of Isfahan, Isfahan, Iran.
3 - Professor, Department of Microbiology, Faculty of Sciences, University of Isfahan, Isfahan, Iran.

Received: 2014-08-28 Accepted : 2014-11-27 Published : 2015-09-01

Keywords: Zinc oxide nanoparticles, Pseudomonas stutzeri, czcC gene, Zinc ion,

Abstract :

Background & Objectives: Environmental pollution to toxic heavy metals is one of global environmental problems. Microorganisms perform several mechanisms to reduce the intracellular concentration of toxic pollutants. The objective of this study was to identify zinc resistance determinant (czcC gene) in zinc resistant Pseudomonas stutzeri SEE-1 isolated from soil. Materials & Methods: The strain was performed on a P. stutzeri strain isolated previously from soil by culture on MTBE (Methyl Tertiary Butyl Ether). The maximum tolerable concentrations of zinc ions and zinc oxide nanoparticles were determined. PCR amplification was used to investigate the czcC gene. The nucleotide sequence was translated into protein sequence and its sequence was compared with other similar sequences in other zinc resistant bacteria. Results: The highest tolerable concentrations of zinc ion and zinc nanooxide was measured at 4mM and 600 µg ml-1, respectively. The primers used for the amplification of the Zn-resistance gene (czcC) yielded a approximately 250 bp band. The comparison of czcC with other sequences in the gene bank database demonstrated 100% similarities with czcC gene in P. stutzeri 1501.          Conclusion: The results of this study showed that soils contaminated with heavy metals are potential sources for the isolation of resistant strains to heavy metals and metal oxide nanoparticles. Understanding the genetic basis of bacterial resistance to heavy metals can improve application of these natural mechanisms to provide a safer environment for all living things.