Isolation and Identification of Pediococcus Species from Raw Milk of Iranian One Humped Camel and Evaluation of Their Antibacterial Properties

Subject Areas : Food Microbial Contamination

Nafiseh Davati ¹ , Farideh Tabatabaei yazdi ² , Saeed Zibaei ³

1 - PhD student of Food Microbiology, Department of Food Science Industry, Ferdowsi University of Mashhad, Mashhad, Iran.
2 - Department of Food Science Industry, Ferdowsi University of Mashhad, Mashhad, Iran.
3 - Razi Vaccine & Serum Research Institute, Mashhad, Iran.

Received: 2015-10-30 Accepted : 2015-12-23 Published : 2015-12-01

Keywords: camel, Antibacterial activity, Raw Milk, Pediococcus,

Abstract :

Iran is a country with vast arid desert and camels have many adaptations that allow them to live successfully in desert conditions. Lactic Acid Bacteria such as Pediococcus play an important role in quality of fermented products of camel milk. A total of three samples of raw milk of Iranian one humped camel (Camelus dromedarius) were collected from Golestan province in Iran under aseptic conditions. Among isolated bacteria, only six isolates were phenotypically characterized as pediococcus species. Six bacterial isolates were identified by amplification of the 16S rRNA gene by Polymerase Chain Reaction (PCR) and were then grouped by the Amplified Ribosomal DNA Restriction Analysis (ARDRA) method. Based on restriction analysis of 16S rRNA gene, the isolates of pediococcusgenus were grouped into one ARDRA pattern that were identified by ribosomal DNA sequencing as Pediococcus pentosaceus. The antimicrobial activity of Pediococcusisolatesagainst Salmonella typhi PTCC 1609 and Bacillus cereus ATCC 10876 was examined by the Spot on lawn method. The results showed that antimicrobial activity of P. pentosaceus isolated from raw milk of Iranian camel was remarkable was remarkable.