Investigating the effect of phage cocktail against antibiotic-resistant Pseudomonas aeruginosa
Subject Areas :Behzad Hemati 1 * , Maryam Sadeghiyani 2
1 - عضو هیات علمی گروه علوم دامی دانشگاه آزاد اسلامی واحد کرج
2 - .
Keywords: Keywords: Pseudomonas aeruginosa, lytic bacteriophage, phage cocktail, resistant strains,
Abstract :
Introduction: Today, with the increase in the resistance of pathogenic bacteria to all kinds of antibiotics and the spread of antibiotic-resistant strains and the increase in the percentage of deaths resulting from them, it seems very necessary to find new solutions to deal with resistant strains. One of these solutions is the use of phage cocktails (a collection of two or more bacteriophages) that specifically and without harming the cells of the human or animal host body cause the destruction of the bacteria causing the infection. The aim of this study is to isolate and investigate the effect of lytic bacteriophages against Pseudomonas aeruginosa from hospital wastewater. Methods: In this study, the wastewater sample was taken from the wastewater inlet of one of the hospitals located in Karaj city and transferred to the research laboratory of the university. After preparing the phage cocktail, two strains of Pseudomonas aeruginosa ATCC27853 and RTCC1474 were used. The presence of bacteriophage and the sensitivity of the target bacteria to bacteriophage were confirmed by two-layer culture and spot test methods and the observation of plaques and clear halos in the environment, and imaging was done using an electron microscope. Results: The presence of two phages with the size of 50 and 80 nm was confirmed by observing with an electron microscope, which corresponded to the characteristics of two families of phages called tectoviride and cystoviride. Conclusion: Considering the resistance of both bacterial strains to antibiotics, these phages can be used to eliminate the pathogenic and antibiotic-resistant Pseudomonas aeruginosa bacteria.
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