Screening and cloning of the gene encoding dextranase enzyme from soil Streptomyces for use in dental cases
Subject Areas :
Ashkan Goodarzi
1
,
kumarss amini
2
*
,
Mohaddeseh Larypoor
3
1 - islamic azad university tehran north branch
2 - Assistant Professor, Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran
3 - Department of Microbiology, North Branch of Islamic Azad University, Tehran, Iran
Keywords: Key words: Soil Streptomyces, Dextranase, Cloning ,
Abstract :
Introduction: This study aimed to explore the molecular, cloning, and kinetic properties of the dextranase enzyme produced by Streptomyces isolated from soil, for potential applications in dentistry. Dextranase is an enzyme that breaks down dextran, a polysaccharide, and is important in both therapeutic and industrial fields, including use in blood substitutes, vasodilators, and the sugar industry. Methods: Microbial enzymes, such as those from soil bacteria, are preferred over plant and animal-derived enzymes due to their variety, lower cost, and greater stability. Soil bacteria, in particular, are abundant sources of biologically active compounds with minimal toxicity, making them suitable for human treatment. Results: The study isolated 12 Streptomyces strains from soil samples. After biochemical and molecular analysis, three strains, identified as Streptomyces quilicolor, were found to contain the dextranase gene. The optimal conditions for dextranase production were determined to be a pH of 5.2 and a temperature range of 55-65°C. The dextranase gene was successfully cloned into Escherichia coli using a TA cloning technique, and its expression was confirmed through Real-Time PCR. Conclusion: The results suggest that Streptomyces from soil, with its high potential for dextranase production, could serve as a valuable resource for future industrial and therapeutic applications. The isolated strains are promising candidates for the development of efficient dextranase-producing strains, particularly for use in dental treatments and other clinical fields.
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