A Novel Approach for Water Conservation and Yield Enhancement in Rice: Genetic Engineering with OsNAC5 and EPSPS Genes
Subject Areas :Seyyed Mohammad Mousavi pakza 1 , Elahe Moatamed 2 , Nasrin Soltani 3 , Mohaddeseh Mohsenpour 4 , Aliakbar Ebadi 5 , مطهره محسن پور 6 *
1 - Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran
2 - Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran
3 - Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran
4 -
5 - Rice Research Institute of Iran (RRII), Agricultural Research Education and Extension Organization (AREEO) Rasht, Iran
6 - پژوهشکده بیوتکنولوژی کشاورزی کرج
Keywords: Keywords: Rice Genetic Engineering, EPSPS, OsNAC5, Combined Gene Transfer, Reduced Water Use ,
Abstract :
Introduction: Utilizing scientific approaches to address weed-related challenges in rice cultivation could revolutionize farming methods, eliminating the need for flooded fields and plot-based systems while significantly reducing production costs. This study focuses on leveraging advanced technologies by combining herbicide tolerance genes with genes conferring drought tolerance or yield improvement. Methods: To achieve this, a multigenic construct was designed, incorporating a herbicide tolerance gene alongside OsNAC5, a gene associated with drought tolerance, improved yield, and root architecture modification. The coding sequence of OsNAC5 was codon-optimized and placed under the control of the RCc3 promoter and tahsp17 terminator, alongside an EPSPS gene cassette within the T-DNA region of an Agrobacterium-based vector. The resulting genetic construct was introduced into rice using Agrobacterium tumefaciens, followed by continuous selection and regeneration processes. Results: Polymerase chain reaction (PCR) analysis using gene- and construct-specific primers confirmed the presence of transgenes in regenerated plants grown on selective media. Six independent transformation events of the recombinant construct, recombinant pUhErN5, were obtained. These events were further characterized by inverse PCR to distinguish individual events and identify transgene insertion sites. Conclusion: The development of these transgenic rice lines represents a significant step toward addressing the country’s climatic and resource constraints, supporting food security and self-sufficiency. The adoption of such technologies could contribute to the sustainability of rice production in Iran, ensuring resilience against environmental challenges.
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