Application of HPTLC in the Assessment of the Extraction Process of Stevia Rebaudiana Using Ultrasound Waves

Subject Areas : Microbiology

M. Rouhani ¹ (Assistant Professor of the Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran.)
Z. Mirjafari ² (Assistant Professor of the Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran.)
H. Rezapour ³ (MSc Student of the Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran.)
J. Mokhtari ⁴ (Assistant Professor of the Department of Chemistry, Science and Research Branch, Islamic Azad University, Tehran, Iran.)

Received: 2019-12-26 Accepted : 2019-12-26 Published : 2019-12-22

Keywords: HPTLC, Stevioside, Stevia rebaudiana, Ultrasound,

Abstract :

Introduction: In the recent decades, the increase in population, decrease in physical activities and consumption of improper food to fulfill the nutritional need have caused various diseases namely diabetes. The extract from the leaves of Stevia Rebaudiana plant might be employed as a natural sugar substitute. The aim of the present study is to employ ultrasound waves to optimize the extraction process from the leaves of Stevia Rebaudiana and use High Performance Thin Layer Chromatography (HPTLC) to analyze the extract. Materials and Methods: Water, water-methanol mixture, methanol, water-ethanol mixture and ethanol was applied for extraction as solvent. The extraction was carried out by soxhlet and ultrasound waves at various times and power condition according to the official procedures. Results: The results indicated that water was the most suitable solvent among the solvents employed. The results also showed that the application of ultrasound with power of 90 W for 2 minutes resulted better extraction (811.61 g/mlμ) as compared to the soxhlet method (795.70 g/mlμ). Conclusion: The present study indicated that ultrasound application might be a suitable, fast and safe method for extraction of Stevioside sweetener from Stevia Rebaudiana.

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