Biochemical Effects of Fructose Diet on Obesity in Adult Male NMRI Mice

Subject Areas : Microbiology

Zahra Poyamanesh ¹ (دانش آموخته دکتری زیست شناسی، دانشکده علوم پایه، واحد علوم و تحقیقات تهران، دانشگاه آزاد اسلامی، تهران، ایران)
Parichehr yaghmaei ² (دانشیار گروه زیست شناسی، دانشکده علوم پایه، واحد علوم و تحقیقات تهران، دانشگاه آزاد اسلامی، تهران، ایران)
Azadeh Ebrahim Habibi ³ (دانشیار مرکز تحقیقات غدد درون ریز و متابولیسم دانشگاه علوم پزشکی تهران، تهران، ایران)
Mahsa Mohammad Amoli ⁴ (دانشیار مرکز تحقیقات غدد درون ریز و متابولیسم دانشگاه علوم پزشکی تهران، تهران، ایران)

Received: 2015-11-11 Accepted : 2015-11-11 Published : 2015-12-22

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Abstract :

Introduction: excessive use of artificial sweeteners along with increased body mass raises the risk of cardiovascular diseases, asthma, arthritis, type2 diabetes, stroke, high blood pressure and other diseases and leaves adverse metabolic effects. This study investigates the effect of fructose on obesity and its effects on biochemical parameters in adult male mice (NMRI). Materials and Methods: Investigation was carried out in two stages. In the first stage of the treatment which lasted 8 weeks (n=6) the control group received water and rodent food, the obese group had a high-fat diet and the experimental group1received high-fat diet (1382 Kcal) along with 20% of fructose gavage.In the second stage of the treatment which lasted 12weeks, the control group 2, obese group 2 (received rodent food for the last four weeks) and experimental group 2 (received fructose with the same dosage for the last4 weeks) and the study continued for 12 week. The weights were measured every week and the amount of serum fructose and biochemical parameters were measured by sandwich Elisa method. Results: Obese and the experimental group1showed more weight gain as compared to the control 1 and statistically the results were significant (P≤0.001) but experimental 1 showed less weight as compared to the obese 1 but was not significant. Also the experimental group 2 showed higher weight gain as compared to control 2 which was statistically significant (P≤0.001). Fructose levels were higher in all the groups as compared to the control group. The same result was observed for glucose, total cholesterol, TG, LDL with the decrease of HDL. Conclusion: The extra calorie intake is stored in the form of fat leading to weight gain and it is indicated that the receiving of 20% fructose does not play a strong role in gaining weight.